##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926446_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 712672 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.921562794665707 27.0 10.0 31.0 2.0 31.0 2 16.851342553095954 22.0 2.0 31.0 2.0 31.0 3 20.524161184949037 25.0 15.0 31.0 2.0 31.0 4 22.917154034394503 28.0 7.0 35.0 2.0 37.0 5 23.51881651025998 30.0 10.0 35.0 2.0 37.0 6 24.27302321404517 31.0 10.0 35.0 2.0 37.0 7 24.024670535674193 31.0 10.0 35.0 2.0 37.0 8 24.174544531004447 31.0 16.0 35.0 2.0 37.0 9 26.992573020968972 32.0 17.0 37.0 2.0 39.0 10 27.724132560280186 32.0 25.0 38.0 2.0 39.0 11 24.776943671141844 30.0 10.0 37.0 2.0 39.0 12 25.914311211889903 31.0 17.0 37.0 2.0 39.0 13 22.47955440932154 29.0 2.0 37.0 2.0 39.0 14 25.940901284181223 31.0 17.0 38.0 2.0 40.0 15 27.090308023887566 31.0 19.0 38.0 2.0 40.0 16 27.476804196039694 32.0 22.0 39.0 2.0 40.0 17 26.776362758744554 31.0 17.0 38.0 2.0 40.0 18 26.863769026985768 31.0 17.0 38.0 2.0 40.0 19 27.253141697723496 31.0 22.0 39.0 2.0 40.0 20 27.535080654214 32.0 25.0 39.0 2.0 40.0 21 25.306166090431503 31.0 10.0 38.0 2.0 40.0 22 26.289297180189482 31.0 17.0 38.0 2.0 40.0 23 26.29476954335234 31.0 10.0 39.0 2.0 40.0 24 25.308790018409592 31.0 2.0 39.0 2.0 40.0 25 25.107766265547124 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.006332905331632 1101 2 10.822458255894091 1101 3 7.261231607699621 1101 4 8.744923496494728 1101 5 8.482138759342075 1101 6 8.030206825571806 1101 7 7.569789051324204 1101 8 6.089827180318338 1101 9 5.912656970461864 1101 10 5.462414678309152 1101 11 8.270356393401304 1101 12 5.866382590616162 1101 13 10.6037908923612 1101 14 7.654654559464554 1101 15 7.186053391008819 1101 16 6.477451899821567 1101 17 2.3873507253004007 1101 18 4.9779447290437595 1101 19 5.6894170517265685 1101 20 3.469410915121678 1101 21 1.221580060373018 1101 22 4.7992322438223916 1101 23 5.975208437320038 1101 24 7.347656011343105 1101 25 7.708816332039888 1102 1 5.877536489476878 1102 2 10.934480402468413 1102 3 7.067331045557481 1102 4 8.44708924364815 1102 5 8.399531254548869 1102 6 7.182978408047578 1102 7 6.9895872801199666 1102 8 7.205260655817547 1102 9 6.169795262645348 1102 10 5.528885703859878 1102 11 8.273818589016333 1102 12 5.705862967812234 1102 13 10.302307280565348 1102 14 7.599096395360782 1102 15 5.490983547130426 1102 16 5.940882662430639 1102 17 6.640175130877481 1102 18 6.740536191265463 1102 19 5.400973572444965 1102 20 5.841097732823286 1102 21 5.06023352778573 1102 22 6.082692091744029 1102 23 6.31492423599229 1102 24 7.825325534370059 1102 25 7.71794103368719 1103 1 5.216927857025222 1103 2 9.705699940699528 1103 3 6.6440163227618 1103 4 8.28900071037948 1103 5 7.424774418256803 1103 6 6.939775822911454 1103 7 7.043775507708414 1103 8 6.607875575832651 1103 9 5.469046909490135 1103 10 4.6589167594944705 1103 11 5.736923137714641 1103 12 4.351722644841541 1103 13 8.774810227654797 1103 14 6.879044523950746 1103 15 5.912677288362467 1103 16 5.825597926476014 1103 17 3.7001560612813584 1103 18 2.5808793912807495 1103 19 4.415989396783274 1103 20 4.766970773066458 1103 21 7.121662798627366 1103 22 2.6218878872077873 1103 23 4.780669798555255 1103 24 6.160494442625801 1103 25 2.3246638899884893 1104 1 5.415856714855444 1104 2 10.227220160655996 1104 3 6.585834782302797 1104 4 7.33698698855822 1104 5 6.928170505373821 1104 6 6.759571904936884 1104 7 6.679312860848015 1104 8 6.480303370794527 1104 9 5.302339510144403 1104 10 3.909240075134548 1104 11 7.092578615623527 1104 12 4.183842908076482 1104 13 8.766489305059615 1104 14 6.175289480099472 1104 15 5.586847041802464 1104 16 5.140150312519985 1104 17 5.699255487205164 1104 18 3.3438177449167767 1104 19 4.125924988234505 1104 20 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18 6.471223812829173 2205 19 4.940321210858574 2205 20 5.205034784922759 2205 21 -0.9417361943989313 2205 22 3.7055487999807184 2205 23 4.909383140956766 2205 24 6.76656800652092 2205 25 7.146143034872921 2206 1 4.288433745667767 2206 2 9.11072156121622 2206 3 6.2942941660562965 2206 4 1.9562680343098542 2206 5 5.853903465224427 2206 6 6.231504678046129 2206 7 6.3510308813895655 2206 8 6.338267889935516 2206 9 4.791433814532827 2206 10 3.8032550144436037 2206 11 3.4439231534386536 2206 12 4.79837138613437 2206 13 8.120718996936365 2206 14 0.41874419531889373 2206 15 2.8233082929695996 2206 16 3.685351059485434 2206 17 4.624755020351756 2206 18 -1.1229236182951752 2206 19 1.021209768813403 2206 20 2.610475340891984 2206 21 3.7905062975345487 2206 22 -1.966304637596842 2206 23 -2.9604871742579277 2206 24 1.0889445267492626 2206 25 2.940525015513277 2207 1 1.931603468102196 2207 2 -14.20849412505829 2207 3 -6.255669546701533 2207 4 -4.055401178829946 2207 5 1.795017547220862 2207 6 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19 3.2326444854498675 2208 20 4.4847806693344054 2208 21 7.1046277748256905 2208 22 6.051762862881937 2208 23 1.888915162183789 2208 24 3.6212920172105143 2208 25 4.943498454325315 2209 1 3.1552504772663745 2209 2 4.892895899471554 2209 3 5.352388533811574 2209 4 0.6756850897665423 2209 5 5.318497440181542 2209 6 5.746138894774521 2209 7 6.34601781483833 2209 8 4.907314753161593 2209 9 4.579634478281562 2209 10 3.700946002531399 2209 11 -0.26641176711992287 2209 12 3.435607369652878 2209 13 2.733085886455868 2209 14 3.9608454250176415 2209 15 4.404287242194425 2209 16 3.7625081627192074 2209 17 1.4159390262517455 2209 18 3.616400127556396 2209 19 4.282367641610925 2209 20 4.386897190089552 2209 21 6.688459578601677 2209 22 5.42012089909371 2209 23 1.1212667897970334 2209 24 -2.77778883544271 2209 25 0.5553218025846078 2210 1 -2.4174238886977335 2210 2 -14.20849412505829 2210 3 -7.813686593538852 2210 4 -2.096582952762997 2210 5 -0.7308652156721216 2210 6 -1.2859379514758693 2210 7 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-8.828877101377714 2211 20 -7.004247915499178 2211 21 -4.689731974989648 2211 22 -6.214018710567803 2211 23 -8.10237846241876 2211 24 -22.776153512466422 2211 25 -22.595945572722016 2212 1 -0.6978265897917169 2212 2 -14.20849412505829 2212 3 -7.644793224228826 2212 4 -20.290790789219557 2212 5 -20.78452790732459 2212 6 -21.5261423807774 2212 7 -9.909425241745511 2212 8 -9.382394306360982 2212 9 -4.534901027096133 2212 10 -5.02800413982051 2212 11 -5.581473637344388 2212 12 -2.445184557095562 2212 13 -6.037239897464769 2212 14 -3.331059726249734 2212 15 -3.2729827535694973 2212 16 -3.5424386513942814 2212 17 -3.789354630923029 2212 18 -2.6905821256276745 2212 19 -3.192848273309842 2212 20 -3.4098688412665155 2212 21 -1.218145140797965 2212 22 -2.685180866494335 2212 23 -2.669885491508829 2212 24 -1.639916255747238 2212 25 -2.2326462214708584 2213 1 -19.348428999430272 2213 2 -14.20849412505829 2213 3 -8.893137400629783 2213 4 -5.896731383278961 2213 5 -20.78452790732459 2213 6 -11.11525129166849 2213 7 -21.357988726453573 2213 8 -11.02703593456458 2213 9 -8.728274979876026 2213 10 -7.561536288216072 2213 11 -6.649736330156767 2213 12 -5.424800813438527 2213 13 -19.723986885416576 2213 14 -12.729079528229931 2213 15 -7.283253539684253 2213 16 -6.575211937990783 2213 17 -5.107541621077093 2213 18 -5.145369745260812 2213 19 -6.114472622955187 2213 20 -6.0615650396166565 2213 21 -4.391306942612996 2213 22 -5.068102346779526 2213 23 -4.7632089758498495 2213 24 -3.8860545025654325 2213 25 -4.967232701434888 2214 1 -19.348428999430272 2214 2 -14.20849412505829 2214 3 -12.24993558580162 2214 4 -20.290790789219557 2214 5 -20.78452790732459 2214 6 -21.5261423807774 2214 7 -16.74878101767413 2214 8 -14.984289946903717 2214 9 -13.691382661093828 2214 10 -13.587213138556354 2214 11 -14.779252729334157 2214 12 -12.090015900257702 2214 13 -19.723986885416576 2214 14 -16.8931582273204 2214 15 -24.513946608991183 2214 16 -18.50022730890689 2214 17 -24.23328419533452 2214 18 -18.054323038877115 2214 19 -14.804847249282915 2214 20 -13.516701045722618 2214 21 -10.98806950966199 2214 22 -12.041352712501324 2214 23 -12.004386070004662 2214 24 -10.770800193408606 2214 25 -10.584168270794821 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 113833.0 3 2.0 4 633.0 5 2295.0 6 3142.0 7 2966.0 8 2462.0 9 2333.0 10 2176.0 11 2030.0 12 2011.0 13 2192.0 14 3291.0 15 7260.0 16 10677.0 17 11369.0 18 11569.0 19 13367.0 20 15795.0 21 18223.0 22 19001.0 23 20844.0 24 22400.0 25 23210.0 26 19686.0 27 15064.0 28 15986.0 29 18801.0 30 18240.0 31 20938.0 32 27139.0 33 33981.0 34 41871.0 35 48540.0 36 48356.0 37 50819.0 38 40170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.559480138350274 19.02300194303129 12.293502535615522 26.124015383002913 2 13.073477919812786 20.620883382780214 42.96225530367105 23.34338339373595 3 18.66824625552819 24.63223341097666 29.074381711211366 27.625138622283785 4 10.949551287767626 16.747707797945733 40.0856079706209 32.21713294366574 5 11.645814369363263 37.86047682683555 38.069806777319414 12.423902026481771 6 31.223782361404357 38.05558426474135 18.636690995650966 12.08394237820333 7 27.053942549870975 31.56960304093206 23.07322650926341 18.303227899933557 8 23.38053854603494 39.61141421436244 20.640319947565498 16.367727292037117 9 26.508601617359716 15.016694737049113 20.389492359190392 38.08521128640078 10 13.909944579966242 29.623957850032877 36.04180839526695 20.424289174733932 11 33.12584638046138 21.53637357154736 26.4746323442066 18.86314770378466 12 24.147575463033945 24.149808207270112 33.05577841650802 18.646837913187927 13 28.780651170230314 21.970538887435623 25.538129330985154 23.710680611348913 14 19.226608939287917 23.521959158544597 27.965808214180356 29.285623687987126 15 21.148612063675415 34.41504530195286 23.123127459119953 21.31321517525178 16 20.294980735126767 28.056529550062674 30.720829747043837 20.92765996776672 17 20.09857477900054 29.462024174634678 30.233447591556917 20.205953454807865 18 20.165873706425756 27.345807089221196 33.12632347936049 19.36199572499256 19 22.868997124882576 26.69674627800393 27.46477269492442 22.969483902189076 20 23.024796714220788 28.369956901585624 29.014814992000833 19.590431392192755 21 20.979980934223068 26.702647209796876 26.887878565666934 25.429493290313122 22 22.012384960142324 30.154270302325315 27.919189845701187 19.914154891831174 23 20.489181508759163 29.377861249671643 29.468887443582727 20.664069797986468 24 22.48009301235436 27.27534864213065 29.968420208599326 20.276138136915666 25 21.4858061180913 28.235345350745096 29.65220494032501 20.626643590838597 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 636.0 1 636.0 2 1417.0 3 2198.0 4 2198.0 5 2198.0 6 3167.5 7 4137.0 8 4137.0 9 4137.0 10 4756.5 11 5376.0 12 5376.0 13 5376.0 14 6719.5 15 8063.0 16 8063.0 17 8063.0 18 11974.0 19 15885.0 20 15885.0 21 15885.0 22 22943.0 23 30001.0 24 30001.0 25 30001.0 26 40281.0 27 50561.0 28 50561.0 29 50561.0 30 62223.0 31 73885.0 32 73885.0 33 73885.0 34 80205.0 35 86525.0 36 86525.0 37 86525.0 38 89348.5 39 92172.0 40 92172.0 41 92172.0 42 90914.0 43 89656.0 44 89656.0 45 89656.0 46 86600.0 47 83544.0 48 83544.0 49 83544.0 50 75090.5 51 66637.0 52 66637.0 53 66637.0 54 55883.0 55 45129.0 56 45129.0 57 45129.0 58 37022.0 59 28915.0 60 28915.0 61 28915.0 62 22563.0 63 16211.0 64 16211.0 65 16211.0 66 12074.0 67 7937.0 68 7937.0 69 7937.0 70 5647.5 71 3358.0 72 3358.0 73 3358.0 74 2317.0 75 1276.0 76 1276.0 77 1276.0 78 849.5 79 423.0 80 423.0 81 423.0 82 266.0 83 109.0 84 109.0 85 109.0 86 69.0 87 29.0 88 29.0 89 29.0 90 18.5 91 8.0 92 8.0 93 8.0 94 4.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.98030510529388 2 35.96184500022451 3 5.483448206187418 4 13.457382919491714 5 13.060145480669929 6 14.468226617574423 7 9.191044407525482 8 7.515940011674374 9 0.10818441021956805 10 1.408502087917022 11 5.5026716357595085 12 5.732370571595348 13 15.784400116743747 14 1.5411016568631852 15 2.819950832921737 16 2.8372098244353645 17 2.7778557316689865 18 1.4664530106416416 19 1.4160792061425171 20 0.27684544923892057 21 4.324850702707557 22 1.5681828386691212 23 0.11071011629473307 24 9.00217771990481 25 0.11449867540748059 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 712672.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.42607412950193 #Duplication Level Percentage of deduplicated Percentage of total 1 96.69515800399859 87.43763525633477 2 2.3225703251964913 4.200418327943986 3 0.4180649642252166 1.1341192033793104 4 0.16921780439758738 0.6120680689795118 5 0.09908431199214436 0.44799026706361716 6 0.059429248290169615 0.3224372166808472 7 0.03940490336179063 0.24942614987213974 8 0.02660197532302688 0.19244097540409677 9 0.023151392174354142 0.18841405544633869 >10 0.12738779907416475 2.176628417257184 >50 0.010768456079323327 0.6830712208579245 >100 0.008196519481442725 1.4782889351041044 >500 4.821482028304259E-4 0.33934375306611597 >1k 4.821482028304259E-4 0.5377181526101195 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1428 0.200372681963091 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1179 0.16543374792330834 No Hit CTGTAGGACGTGGAATATGGCAAGA 1093 0.1533664855641866 No Hit CTTTAGGACGTGAAATATGGCGAGG 882 0.12375959768308561 No Hit GTCCTACAGTGTGCATTTCTCATTT 879 0.12333864667055812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.4031700417583404E-4 0.0 9 0.0 0.0 0.0 1.4031700417583404E-4 0.0 10 0.0 0.0 0.0 1.4031700417583404E-4 0.0 11 0.0 0.0 0.0 1.4031700417583404E-4 0.0 12 0.0 0.0 0.0 1.4031700417583404E-4 1.4031700417583404E-4 13 0.0 0.0 0.0 1.4031700417583404E-4 1.4031700417583404E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACTAGA 40 4.7556584E-4 20.412842 2 GTATTGA 45 0.0012373548 17.707445 1 CTCTAGG 90 8.8493834E-9 17.707445 1 TAGGACC 350 0.0 16.798346 4 TGTAGGA 830 0.0 16.395857 2 GTATTAA 60 4.710735E-4 15.494016 1 CTGTAGG 855 0.0 15.22219 1 GTCCTAC 835 0.0 14.950599 1 GTATAAA 80 1.6368263E-5 14.940658 1 GGTCTGG 55 0.0047218106 14.487911 1 GTCTTAC 65 8.6445716E-4 14.302169 1 GTTTAAA 85 2.9007966E-5 14.061796 1 GTAGGAC 955 0.0 14.036235 3 AGGACCT 590 0.0 13.741292 5 ACCTACA 100 7.0290025E-6 13.608562 2 ACGAAAT 40 0.007225618 13.588688 12 TCCTACA 980 0.0 13.330836 2 GTTCTAG 60 0.00840276 13.280585 1 GTTTAAT 60 0.00840276 13.280585 1 CCACTAT 60 0.00840276 13.280585 1 >>END_MODULE