FastQCFastQC Report
Thu 26 May 2016
SRR1926446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712672
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA30600.42937003277805214No Hit
GTCCTACAGTGGACATTTCTAAATT28530.40032441291365456No Hit
CTTTAGGACGTGAAATATGGCGAGG24760.34742490233936507No Hit
GTCCTAAAGTGTGTATTTCTCATTT24590.3450395132683759No Hit
GTCCTACAGTGTGCATTTCTCATTT19110.26814579498001884No Hit
CTGTAGGACCTGGAATATGGCGAGA15740.2208589645727628No Hit
CTGAAGGACCTGGAATATGGCGAGA13050.18311369044946343No Hit
GAATATGGCAAGAAAACTGAAAATC8890.12474181671231646No Hit
GTCCTTCAGTGTGCATTTCTCATTT8620.12095325759956894No Hit
ACCTGGAATATGGCGAGAAAACTGA7150.10032665798572135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGT902.1609594E-914.834024
CCGTCCC456.7527196E-414.7787089
ATAGGAC1006.184564E-1014.3062533
TAGGACC5300.014.0343434
AATCCCG1950.013.60455719
GATATAC3500.013.3487321
GGCGAGG7750.013.20331519
AGGACCT8900.013.0720345
TAGGACT2600.012.8371334
GGACCTG9000.012.8208326
TGTAGGA16050.012.5410582
CTGTAGG15550.012.3860541
ATTCCCG701.1095697E-412.19018411
AGGACTT2050.012.0946365
AATGTCC2750.011.7461948
CTATAGG1302.4574547E-911.7351491
GTAGGAC16450.011.7117053
CCTTACA907.1081486E-611.6594182
AGGACGT15700.011.540575
GGACGTG15450.011.480426
Produced by FastQC (version 0.11.5)