FastQCFastQC Report
Thu 26 May 2016
SRR1926445_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926445_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741545
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA8900.12001968862307749No Hit
GCTTACTCTGCGTTGATACCACTGC7530.1015447477900869No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG501.0895607E-418.7684941
GTCCTAC4000.015.752131
AATACTT507.317497E-414.6218235
TCCTACA4450.014.5277572
GCTCTAT751.4613473E-414.2998051
CTAGGAC857.821418E-714.1934233
TTTAGAA1101.1125885E-613.7550032
GTAGGAC3700.013.635453
TTTAGGA2850.013.5136872
TAGGACG4550.013.4435744
GGACGTG4350.013.4390926
TGTAGGA4000.013.4111292
ATTTAGA1008.208057E-613.4060671
GTCTAGG802.526553E-413.4060661
ACGTGAA2550.013.403028
GTCCTAA2250.013.1081541
AGGACGT4400.013.0551995
TACTGGC853.394515E-412.9458852
CGTGAAA2700.012.9414149
GGCGAGG2500.012.93131219
Produced by FastQC (version 0.11.5)