##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926445_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 741545 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.85263200480079 27.0 10.0 31.0 2.0 31.0 2 16.74241617164164 22.0 2.0 31.0 2.0 31.0 3 20.48678097755362 25.0 15.0 31.0 2.0 31.0 4 22.76235966799048 28.0 7.0 35.0 2.0 37.0 5 23.36768233890054 30.0 7.0 35.0 2.0 37.0 6 24.12390751741297 31.0 8.0 35.0 2.0 37.0 7 23.98991430054818 31.0 10.0 35.0 2.0 37.0 8 24.24019311033046 31.0 17.0 35.0 2.0 37.0 9 27.092250638868848 32.0 18.0 38.0 2.0 39.0 10 27.83819997437782 32.0 26.0 38.0 2.0 39.0 11 24.80457827913343 30.0 10.0 37.0 2.0 39.0 12 26.020840272673944 31.0 17.0 37.0 2.0 39.0 13 22.523413953300203 29.0 2.0 37.0 2.0 39.0 14 26.038945714690275 31.0 17.0 38.0 2.0 40.0 15 27.166997282700308 31.0 19.0 38.0 2.0 40.0 16 27.55704643683121 32.0 23.0 39.0 2.0 40.0 17 26.878950030004923 31.0 17.0 39.0 2.0 40.0 18 26.97236715236432 31.0 18.0 38.0 2.0 40.0 19 27.38223573754796 31.0 23.0 39.0 2.0 40.0 20 27.63958357213655 32.0 25.0 39.0 2.0 40.0 21 25.426616051621952 31.0 10.0 38.0 2.0 40.0 22 26.400857668786117 31.0 17.0 38.0 2.0 40.0 23 26.410701980324863 31.0 11.0 39.0 2.0 40.0 24 25.396230842362904 31.0 2.0 39.0 2.0 40.0 25 25.19631580012002 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.318862082087559 1101 2 11.081395055281146 1101 3 7.500269968594456 1101 4 9.013016449179165 1101 5 8.70640549883569 1101 6 8.354945954527807 1101 7 8.090784220379415 1101 8 6.50316418114776 1101 9 6.496663953467483 1101 10 6.152964781113365 1101 11 8.841704328968063 1101 12 6.78994802502686 1101 13 11.327135370403724 1101 14 8.593528314331468 1101 15 7.687732169524921 1101 16 7.258483856983066 1101 17 3.0935137144316904 1101 18 5.581256109525793 1101 19 6.43247901225908 1101 20 4.196674283011323 1101 21 1.2457286044616502 1101 22 5.188452814837419 1101 23 6.538830981212179 1101 24 7.9773468999581425 1101 25 8.533207249588507 1102 1 5.7353896015195325 1102 2 10.808965493810632 1102 3 6.785762029589158 1102 4 8.185190850994086 1102 5 8.029969765825822 1102 6 7.048098387997243 1102 7 7.058256122365627 1102 8 6.935233473932996 1102 9 5.991308265221576 1102 10 5.159667321544308 1102 11 7.965666117191894 1102 12 5.397474790779892 1102 13 10.03733312865296 1102 14 7.510687706008515 1102 15 5.228503789579374 1102 16 5.594640525772885 1102 17 6.611091943514566 1102 18 6.584806267240086 1102 19 5.504547340741297 1102 20 5.7169322594468 1102 21 5.076870897523488 1102 22 6.065188243076779 1102 23 6.137533105470233 1102 24 7.808048278150174 1102 25 7.546293856688315 1103 1 5.206170942568825 1103 2 9.506267420790358 1103 3 6.561290994565951 1103 4 8.232433501746591 1103 5 7.547787797855076 1103 6 6.976941798289204 1103 7 6.846223048240201 1103 8 6.656650276231709 1103 9 5.835070195818155 1103 10 4.997855779759593 1103 11 5.890940395328013 1103 12 4.7089613179967245 1103 13 8.75624827322667 1103 14 7.047516829725037 1103 15 5.8309712881225835 1103 16 5.663775193323676 1103 17 3.4279585418628677 1103 18 2.0711727349797577 1103 19 4.1729345250200325 1103 20 4.673570192213493 1103 21 7.055513873792908 1103 22 2.369074352345976 1103 23 4.98565535515376 1103 24 6.2900720087347 1103 25 2.292192243169314 1104 1 6.204946669344142 1104 2 10.981516656532321 1104 3 7.411106988490829 1104 4 7.888894646283852 1104 5 8.196633135394265 1104 6 7.820526898665005 1104 7 7.641528459381817 1104 8 7.6738436481186945 1104 9 6.45147107593187 1104 10 5.460248929670993 1104 11 8.597165934202948 1104 12 5.787120088952971 1104 13 9.805847159454451 1104 14 7.695434835060237 1104 15 6.828873229435359 1104 16 6.419623957854316 1104 17 6.738111004039396 1104 18 4.366784822913242 1104 19 5.2292973382684735 1104 20 4.31189116111744 1104 21 3.602929513335713 1104 22 5.682128074331292 1104 23 6.1728450274477 1104 24 7.191382930322796 1104 25 6.039765602205083 1105 1 3.4944203535546663 1105 2 9.415727182848112 1105 3 6.201354666509406 1105 4 5.614900838234316 1105 5 2.2562306586140792 1105 6 4.603034638603084 1105 7 5.552519171456122 1105 8 2.6080541744344856 1105 9 3.407353428873048 1105 10 3.003592582921769 1105 11 6.501190702004802 1105 12 3.735262503813651 1105 13 5.180460162550425 1105 14 3.720202636917822 1105 15 4.034755582376533 1105 16 4.177363895934807 1105 17 4.933931437475927 1105 18 2.513843646442652 1105 19 2.4963252020455613 1105 20 1.8652967338728565 1105 21 2.687294838413113 1105 22 4.008753461328197 1105 23 1.6767149964879522 1105 24 4.579153828155615 1105 25 4.973976128520871 1106 1 6.578848049583598 1106 2 11.245587543197768 1106 3 8.095359689450035 1106 4 9.214228657579525 1106 5 8.835721545327047 1106 6 7.75891687274245 1106 7 7.751885838994003 1106 8 7.365039721005985 1106 9 6.311052085699757 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4.667070532565933 2204 7 6.029983857988629 2204 8 5.914217100131079 2204 9 4.174098301164069 2204 10 3.849703264036318 2204 11 5.468578956648766 2204 12 5.575986671770309 2204 13 3.2533050735411386 2204 14 4.703801224379124 2204 15 4.831374196927619 2204 16 4.734574132002919 2204 17 -0.5432236515333599 2204 18 3.3192761154829 2204 19 4.256941010869092 2204 20 4.5169172834084605 2204 21 6.234970438258312 2204 22 5.531407292597546 2204 23 4.103108185342439 2204 24 5.820226293325895 2204 25 5.876837486880007 2205 1 6.083166542444474 2205 2 10.649953601051976 2205 3 7.4504688408963915 2205 4 8.106173248739442 2205 5 8.098938047753443 2205 6 7.614554491691408 2205 7 7.861735877950608 2205 8 7.469243240815491 2205 9 6.33365450363555 2205 10 5.492027408523011 2205 11 8.24733073896816 2205 12 7.130641495914794 2205 13 10.304823015556345 2205 14 7.554540886926649 2205 15 6.603801715939028 2205 16 6.215939982763377 2205 17 6.6919444805959145 2205 18 6.764101448182519 2205 19 5.224998876838669 2205 20 5.614998319530137 2205 21 -0.6019681311333223 2205 22 4.08017749221732 2205 23 5.428108185342438 2205 24 7.269890623173801 2205 25 7.5764958756632765 2206 1 4.467654762424374 2206 2 9.307368049721177 2206 3 6.4143501062862285 2206 4 1.8841047371533541 2206 5 6.0081734189607126 2206 6 6.4452595298611435 2206 7 6.705641943382755 2206 8 6.651731590084712 2206 9 5.517163650854947 2206 10 4.616675829724109 2206 11 4.398890732151084 2206 12 5.512422867674481 2206 13 8.834988564653965 2206 14 0.949465105754701 2206 15 3.356796820575223 2206 16 4.176303990967369 2206 17 5.3353686494712775 2206 18 -0.6020700012095261 2206 19 1.6363833891534796 2206 20 3.2903258035011973 2206 21 4.125210246990925 2206 22 -1.220638205856858 2206 23 -2.3327882597889342 2206 24 2.039023385675197 2206 25 3.561088114777995 2207 1 1.8109904881823127 2207 2 -14.192631950278823 2207 3 -6.243532367973019 2207 4 -4.52262761831172 2207 5 1.5999233060971747 2207 6 2.5389840750483152 2207 7 3.5094052313245747 2207 8 3.458180001462324 2207 9 0.9236351795900681 2207 10 0.7389309461036646 2207 11 -1.3403745231867852 2207 12 -1.9024362677363484 2207 13 0.9269768873625956 2207 14 -5.532336329457998 2207 15 -1.2286823320465388 2207 16 -0.16814403068306305 2207 17 1.085107520158104 2207 18 0.9296165128987184 2207 19 0.5791249625135713 2207 20 0.3563639846613995 2207 21 -4.2414201500345605 2207 22 -4.603818089156565 2207 23 -1.4636694184163694 2207 24 0.45696584403066964 2207 25 1.1451414952981729 2208 1 5.218102847908462 2208 2 6.682460437008686 2208 3 6.555597845867741 2208 4 8.571789440331706 2208 5 3.6992645999301317 2208 6 6.4900189573626825 2208 7 7.107801092837114 2208 8 7.03844335659819 2208 9 5.577996467276414 2208 10 4.890921852358563 2208 11 6.972755439464731 2208 12 6.410937360055598 2208 13 9.277113854952816 2208 14 3.6764493904914666 2208 15 5.085754509196651 2208 16 5.585840415262343 2208 17 6.322344183832492 2208 18 0.9175657958228847 2208 19 3.7000772128277006 2208 20 4.808846099003436 2208 21 7.401417107696599 2208 22 6.452285664733541 2208 23 2.56691084609632 2208 24 4.044543204818872 2208 25 5.357604185882224 2209 1 2.6897411201024575 2209 2 4.867494631999659 2209 3 5.154182683125779 2209 4 0.4883993882205999 2209 5 4.781392805418168 2209 6 5.416526624338086 2209 7 5.863458937102553 2209 8 4.684588935574116 2209 9 4.2662759119235645 2209 10 3.060858327327459 2209 11 -0.9360986541107295 2209 12 2.671150833162713 2209 13 1.9498932855664535 2209 14 3.425646556657604 2209 15 3.8068291019909104 2209 16 3.526415508585199 2209 17 1.182794940238793 2209 18 3.1893908306727745 2209 19 3.959700108970356 2209 20 3.959579466952764 2209 21 6.259861656612745 2209 22 4.81623719766084 2209 23 0.43870945116522364 2209 24 -3.3512234693323357 2209 25 0.3164438951078523 2210 1 -1.8949369752953125 2210 2 -14.192631950278823 2210 3 -7.489767983317343 2210 4 -1.5528917929345702 2210 5 0.007401900899768776 2210 6 -0.6471627041890322 2210 7 -0.5227427562497802 2210 8 -21.63265789986892 2210 9 -13.100896524994871 2210 10 -6.770288457817987 2210 11 -10.493181184079711 2210 12 -4.320293336507838 2210 13 -5.304895464538333 2210 14 -3.5877085024420694 2210 15 -5.315656018304171 2210 16 -4.697964154420923 2210 17 -4.154073196235348 2210 18 -4.303478437497233 2210 19 -10.032550917938856 2210 20 -6.425663428512067 2210 21 -4.260409321675461 2210 22 -9.48712540607795 2210 23 -7.14987194710789 2210 24 -10.121997423561524 2210 25 -7.349999226696152 2211 1 -0.4204390901597108 2211 2 4.751412813909827 2211 3 0.1788686305703564 2211 4 1.2574493381340872 2211 5 0.6916538370991532 2211 6 -0.38633361911272246 2211 7 -21.37704739201137 2211 8 -13.824504422650696 2211 9 -9.648550454831135 2211 10 -7.746414841479268 2211 11 -10.439487715013904 2211 12 -5.552293828829214 2211 13 -7.62863250139891 2211 14 -5.522919274221998 2211 15 -6.05460327109796 2211 16 -12.27138422631043 2211 17 -8.318606470278365 2211 18 -6.528649294189361 2211 19 -9.750136452160486 2211 20 -7.515795296527589 2211 21 -5.243626059543445 2211 22 -6.786951770152253 2211 23 -8.945512917775066 2211 24 -22.885242456674106 2211 25 -22.664568763119995 2212 1 -1.064131165253631 2212 2 -14.192631950278823 2212 3 -7.940276309068988 2212 4 -20.21017656114649 2212 5 -20.736803397113476 2212 6 -21.477460717434067 2212 7 -10.31075346229891 2212 8 -9.758855982936014 2212 9 -5.281055952030819 2212 10 -5.441770378136205 2212 11 -6.065484042552512 2212 12 -3.18239741369295 2212 13 -6.659152494350236 2212 14 -4.301612613480302 2212 15 -3.9647869452449065 2212 16 -4.174528803300596 2212 17 -4.493540644344861 2212 18 -3.4112001225362683 2212 19 -3.949925502709185 2212 20 -4.0115022453455325 2212 21 -1.9470907638183625 2212 22 -3.21037086235453 2212 23 -3.361668172485036 2212 24 -2.2079261627443927 2212 25 -2.7588179643979487 2213 1 -19.322917107745642 2213 2 -14.192631950278823 2213 3 -8.682094483250594 2213 4 -5.715873318376989 2213 5 -20.736803397113476 2213 6 -11.093586922517327 2213 7 -21.37704739201137 2213 8 -11.027925209246659 2213 9 -8.612917611412616 2213 10 -7.423571275840981 2213 11 -6.492761835419596 2213 12 -5.113323007239334 2213 13 -19.839663676458862 2213 14 -12.690625950248398 2213 15 -7.1908910857191835 2213 16 -6.415931866025126 2213 17 -4.997892664460618 2213 18 -4.96957823377214 2213 19 -5.930725372785599 2213 20 -5.935811956293552 2213 21 -3.899051937946769 2213 22 -5.064535137288516 2213 23 -4.870226608697877 2213 24 -3.8983888195137197 2213 25 -4.668074459877225 2214 1 -19.322917107745642 2214 2 -14.192631950278823 2214 3 -12.610811695826035 2214 4 -20.21017656114649 2214 5 -20.736803397113476 2214 6 -21.477460717434067 2214 7 -17.046331003395082 2214 8 -15.564944455315437 2214 9 -14.394304391426706 2214 10 -14.399260180517164 2214 11 -15.122120411604424 2214 12 -12.866908476659525 2214 13 -19.839663676458862 2214 14 -17.34982595300066 2214 15 -24.604563303072382 2214 16 -18.87002366608344 2214 17 -24.33619240153336 2214 18 -18.487193155861554 2214 19 -15.594565521270262 2214 20 -14.156596884893796 2214 21 -11.693899010956603 2214 22 -12.750638524379882 2214 23 -12.694168556561388 2214 24 -11.479943143622094 2214 25 -11.279877889714696 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 118613.0 3 0.0 4 551.0 5 1965.0 6 2839.0 7 2775.0 8 2387.0 9 2383.0 10 2250.0 11 2015.0 12 2034.0 13 2210.0 14 3448.0 15 7549.0 16 11474.0 17 11964.0 18 12412.0 19 14160.0 20 16918.0 21 19601.0 22 20339.0 23 21754.0 24 23144.0 25 23898.0 26 19803.0 27 15467.0 28 16260.0 29 19098.0 30 18377.0 31 21198.0 32 27611.0 33 35475.0 34 43422.0 35 50408.0 36 51020.0 37 53592.0 38 43131.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.021418876162016 17.963341008845237 11.644026238745218 26.37121387624753 2 14.742670940398481 20.48792954731809 39.74208525456036 25.027314257723067 3 19.181588240430465 24.131938728623226 28.58729711121004 28.09917591973627 4 11.487028680030347 16.886883207283024 38.53573076592184 33.0903573467648 5 12.678743893405347 38.1787579584986 35.926311413666895 13.216186734429161 6 31.61439843615102 36.97175345299819 18.55771683117813 12.856131279672656 7 27.812466990697143 30.732163074510645 23.5027724095043 17.952597525287917 8 24.693603018180067 36.090066325763054 20.767369068328243 18.448961587728636 9 26.22435823877635 15.529710355255252 20.161596252370956 38.08433515359744 10 14.524820792332635 29.098917704773076 34.8942000637852 21.48206143910909 11 33.21489616945008 21.622465077260923 25.277792888616574 19.884845864672418 12 24.212869318425653 24.353487121240295 31.659890881729414 19.773752678604637 13 28.399278548078005 21.05818316504823 25.49414625505258 25.04839203182118 14 20.590251217980878 21.38423492705823 28.713642208349437 29.311871646611447 15 22.967340167453546 30.858581507859977 23.445024606723152 22.729053717963325 16 21.774858603459712 27.76670648623166 27.976697905787557 22.481737004521072 17 21.888938432325286 27.616483795823626 28.570079190784366 21.92449858106672 18 21.24727954560707 27.69323272845311 29.861104453121897 21.19838327281792 19 22.90008623875816 27.10716876788085 26.910736040956568 23.08200895240442 20 23.21211219604555 27.183197641394607 27.254875442914717 22.349814719645128 21 22.56665659392642 26.140031799735098 26.478786526627335 24.814525079711146 22 22.692754326578346 27.333226394218705 27.155680043653096 22.818339235549853 23 22.027699664250587 27.760415049457897 27.91661210344916 22.295273182842354 24 23.720575465975188 26.47836352606725 27.52985571719142 22.27120529076614 25 22.503270470988323 27.149836847711477 27.590219772950338 22.75667290834986 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 593.0 1 593.0 2 1318.5 3 2044.0 4 2044.0 5 2044.0 6 2988.5 7 3933.0 8 3933.0 9 3933.0 10 4467.0 11 5001.0 12 5001.0 13 5001.0 14 5820.5 15 6640.0 16 6640.0 17 6640.0 18 9502.0 19 12364.0 20 12364.0 21 12364.0 22 18272.0 23 24180.0 24 24180.0 25 24180.0 26 32920.0 27 41660.0 28 41660.0 29 41660.0 30 52239.5 31 62819.0 32 62819.0 33 62819.0 34 71325.5 35 79832.0 36 79832.0 37 79832.0 38 86005.0 39 92178.0 40 92178.0 41 92178.0 42 95116.5 43 98055.0 44 98055.0 45 98055.0 46 98270.5 47 98486.0 48 98486.0 49 98486.0 50 90004.0 51 81522.0 52 81522.0 53 81522.0 54 69341.5 55 57161.0 56 57161.0 57 57161.0 58 47197.0 59 37233.0 60 37233.0 61 37233.0 62 29191.0 63 21149.0 64 21149.0 65 21149.0 66 15703.5 67 10258.0 68 10258.0 69 10258.0 70 7253.5 71 4249.0 72 4249.0 73 4249.0 74 2875.0 75 1501.0 76 1501.0 77 1501.0 78 995.5 79 490.0 80 490.0 81 490.0 82 318.5 83 147.0 84 147.0 85 147.0 86 91.0 87 35.0 88 35.0 89 35.0 90 22.0 91 9.0 92 9.0 93 9.0 94 6.0 95 3.0 96 3.0 97 3.0 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.266086346749017 2 36.68152303636327 3 5.791691670768463 4 13.788778833381658 5 13.709619780323514 6 14.871922809809249 7 9.356950690787476 8 7.672359735417271 9 0.10923140200527277 10 1.479074095301027 11 5.799108617818203 12 5.729524169133363 13 16.25997073677255 14 1.6261993540513386 15 2.9429097357544047 16 3.0298902966104553 17 2.917961822950731 18 1.541106743353404 19 1.4854122136889871 20 0.28656386328543787 21 4.499120080372735 22 1.6395498587408721 23 0.10977081633616302 24 9.301795575453951 25 0.11138905932883372 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 741545.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.43214020137066 #Duplication Level Percentage of deduplicated Percentage of total 1 97.14445654321906 90.76414483532004 2 2.1558052951622115 4.02843005168906 3 0.314678164903771 0.8820316326479749 4 0.12898728481246965 0.48206232315171166 5 0.06914704927363642 0.32302784011227414 6 0.040228772097614 0.22551961648519556 7 0.02892594020215944 0.18918287502972372 8 0.019423612736142615 0.14518317667043243 9 0.014399982676107915 0.12108790802622807 >10 0.0721125791132212 1.2042605376104372 >50 0.006953199132044214 0.4688451611485092 >100 0.004289870415017805 0.7944365737275184 >500 5.917062565005529E-4 0.37178746838098325 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGA 890 0.12001968862307749 No Hit GCTTACTCTGCGTTGATACCACTGC 753 0.1015447477900869 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.3485358272255897E-4 0.0 2 0.0 0.0 0.0 1.3485358272255897E-4 0.0 3 0.0 0.0 0.0 1.3485358272255897E-4 0.0 4 0.0 0.0 0.0 1.3485358272255897E-4 0.0 5 0.0 0.0 0.0 1.3485358272255897E-4 0.0 6 0.0 0.0 0.0 1.3485358272255897E-4 0.0 7 0.0 0.0 0.0 1.3485358272255897E-4 0.0 8 0.0 0.0 0.0 1.3485358272255897E-4 0.0 9 0.0 0.0 0.0 1.3485358272255897E-4 0.0 10 0.0 0.0 0.0 1.3485358272255897E-4 0.0 11 0.0 0.0 0.0 1.3485358272255897E-4 0.0 12 0.0 0.0 0.0 1.3485358272255897E-4 0.0 13 0.0 0.0 0.0 1.3485358272255897E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAAG 50 1.0895607E-4 18.768494 1 GTCCTAC 400 0.0 15.75213 1 AATACTT 50 7.317497E-4 14.621823 5 TCCTACA 445 0.0 14.527757 2 GCTCTAT 75 1.4613473E-4 14.299805 1 CTAGGAC 85 7.821418E-7 14.193423 3 TTTAGAA 110 1.1125885E-6 13.755003 2 GTAGGAC 370 0.0 13.63545 3 TTTAGGA 285 0.0 13.513687 2 TAGGACG 455 0.0 13.443574 4 GGACGTG 435 0.0 13.439092 6 TGTAGGA 400 0.0 13.411129 2 ATTTAGA 100 8.208057E-6 13.406067 1 GTCTAGG 80 2.526553E-4 13.406066 1 ACGTGAA 255 0.0 13.40302 8 GTCCTAA 225 0.0 13.108154 1 AGGACGT 440 0.0 13.055199 5 TACTGGC 85 3.394515E-4 12.945885 2 CGTGAAA 270 0.0 12.941414 9 GGCGAGG 250 0.0 12.931312 19 >>END_MODULE