FastQCFastQC Report
Thu 26 May 2016
SRR1926445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741545
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA24720.3333580564901658No Hit
GCTTACTCTGCGTTGATACCACTGC14850.20025757034300007No Hit
CTGTAGGACGTGGAATATGGCAAGA10220.13782036154245528No Hit
GTCCTACAGTGGACATTTCTAAATT10010.13498843630528154No Hit
CTGCTTACTCTGCGTTGATACCACT9890.13337019331261082No Hit
CTTTAGGACGTGAAATATGGCGAGG9610.12959429299637917No Hit
GTCCTAAAGTGTGTATTTCTCATTT9220.12433500327019938No Hit
GTCCTACAGTGTGCATTTCTCATTT8600.11597408114140072No Hit
GCGTTGATACCACTGCTTACTCTGC7470.10073562629375156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG250.00589002619.0769391
GGGTTAG500.001453299313.3538571
GTACTAG655.2422925E-513.207111
AAAGGCG603.9414287E-412.7214155
CTAACCC550.002966841212.1439943
AGGGTAT550.00296986312.1423446
GGATTGT550.00296986312.1423446
GTATAGT802.7456232E-511.9230861
TGGATTG657.7298004E-411.7428455
AATGTCC1302.6102498E-911.6904348
TAGGACC2700.011.6612974
AGGACGT5400.011.4846115
GTCCTAG1001.8335086E-611.4461631
GGACGTG5050.011.3351446
AAGGTGT1354.4037733E-911.3071566
TAGGACG5450.011.2041824
GACGTGA2550.011.1761927
GTTAGAG600.005682316611.1319943
GAACTGC600.005688068511.1304816
AGGACCT4650.011.0799435
Produced by FastQC (version 0.11.5)