FastQCFastQC Report
Thu 26 May 2016
SRR1926444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343311
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG451.9374693E-623.6191121
GCCCTGA300.002477896222.1429181
GTGTAGG659.955511E-820.4396151
TATTAGT350.00524625219.4540122
GTCTAGG450.001231049317.7143341
TGTAGGA1750.016.3413682
TAGGACC557.189681E-515.536344
CACTGAC400.003336732315.2614277
AGACTGA559.038478E-515.1255386
TAGGACT506.155466E-414.9537274
TCCACGT400.00444277114.6187818
GTCCTAC2050.014.2578781
TTCCACG508.8994676E-414.24399957
ACTGACC551.5703023E-414.1757898
TTAGGAC550.001085041413.8747973
AATACTC450.006285533313.873355
CTATACT652.973918E-413.1461324
TAGGACA901.9929466E-613.0548414
CTTAATC600.00209018312.7185643
GTAGGAC1800.012.7185633
Produced by FastQC (version 0.11.5)