##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926444_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 343311 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.665201522817505 27.0 10.0 31.0 2.0 31.0 2 16.69217123832327 22.0 2.0 31.0 2.0 31.0 3 20.358232040336606 25.0 15.0 31.0 2.0 31.0 4 22.62554360332177 28.0 7.0 35.0 2.0 37.0 5 23.216780120648625 30.0 7.0 35.0 2.0 37.0 6 23.995520096938346 31.0 8.0 35.0 2.0 37.0 7 23.808794358467978 31.0 10.0 35.0 2.0 37.0 8 24.00309340510499 31.0 12.0 35.0 2.0 37.0 9 26.79070289038219 32.0 17.0 37.0 2.0 39.0 10 27.526309963852018 32.0 25.0 38.0 2.0 39.0 11 24.621299637937614 30.0 8.0 37.0 2.0 39.0 12 25.782238262100545 31.0 17.0 37.0 2.0 39.0 13 22.394682955104848 28.0 2.0 37.0 2.0 39.0 14 25.793053528724684 31.0 17.0 38.0 2.0 40.0 15 26.878247419977804 31.0 17.0 38.0 2.0 40.0 16 27.244078983778557 32.0 19.0 39.0 2.0 40.0 17 26.577112297596056 31.0 17.0 38.0 2.0 40.0 18 26.65903218947252 31.0 17.0 38.0 2.0 40.0 19 27.06935111313066 31.0 19.0 39.0 2.0 40.0 20 27.3077297261084 31.0 23.0 39.0 2.0 40.0 21 25.144577365712138 30.0 10.0 38.0 2.0 40.0 22 26.08393264416229 31.0 11.0 38.0 2.0 40.0 23 26.094278948242263 31.0 8.0 39.0 2.0 40.0 24 25.15186521841712 31.0 2.0 39.0 2.0 40.0 25 24.95142887935429 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.562229216521299 1101 2 10.320192609488885 1101 3 6.994805750813715 1101 4 8.184524915828714 1101 5 7.874505636612994 1101 6 7.450562969123695 1101 7 7.27318212010281 1101 8 5.7030192490266245 1101 9 5.778543032296227 1101 10 5.113490703409752 1101 11 7.441815988244617 1101 12 5.445026330817196 1101 13 9.952106598934005 1101 14 7.256534950500612 1101 15 6.488953428293129 1101 16 6.0290549416144685 1101 17 1.806942745488552 1101 18 4.373391524344029 1101 19 5.3147532941863105 1101 20 3.117341534868981 1101 21 -0.07752912832975412 1101 22 4.143012496923689 1101 23 5.408714639718383 1101 24 6.856974571277515 1101 25 7.341683783685777 1102 1 5.491184018781187 1102 2 10.3671982592064 1102 3 6.751246428779815 1102 4 7.736530565546229 1102 5 7.77566382870339 1102 6 6.551579918276239 1102 7 6.600639747221454 1102 8 6.540279136032275 1102 9 5.74215885150527 1102 10 5.042106522618791 1102 11 7.774697344176822 1102 12 5.129433110478214 1102 13 9.759874960515926 1102 14 7.214077323381968 1102 15 5.379716140157534 1102 16 5.824083190202039 1102 17 6.5825642144151075 1102 18 6.481159885925951 1102 19 5.2754877574631465 1102 20 5.461494077241863 1102 21 4.671340928167421 1102 22 5.742871253985836 1102 23 6.193516899605388 1102 24 7.5686129893566125 1102 25 7.166570789335495 1103 1 5.388366872949057 1103 2 9.983719344414936 1103 3 6.677775034090164 1103 4 8.382403186590945 1103 5 7.822895852767715 1103 6 7.3973889076902495 1103 7 7.771612154232503 1103 8 6.910487963474861 1103 9 6.189714818416817 1103 10 5.474986721612257 1103 11 6.102839879029602 1103 12 5.040111655049277 1103 13 9.626879067648456 1103 14 7.475186827633717 1103 15 6.682076295187329 1103 16 6.260272233992168 1103 17 4.24595867267854 1103 18 3.1386076790653057 1103 19 5.091146923310312 1103 20 5.372068497326321 1103 21 7.592721156084355 1103 22 2.673188833669993 1103 23 5.249408610139316 1103 24 6.698294252733717 1103 25 2.7631115425026174 1104 1 5.188944114699222 1104 2 10.175476639499603 1104 3 6.512394175251014 1104 4 6.78821730596805 1104 5 7.247651402958116 1104 6 6.573842711888968 1104 7 7.080513309813423 1104 8 7.053882057172391 1104 9 5.786946033368039 1104 10 4.951523929561951 1104 11 7.590883512360374 1104 12 4.944126009273788 1104 13 9.114352043735721 1104 14 6.80719411448775 1104 15 5.943278187135569 1104 16 5.555828789417255 1104 17 5.801224631876551 1104 18 3.7591096379560334 1104 19 4.44299552355394 1104 20 3.244817419113353 1104 21 2.9423529724205117 1104 22 4.816297598745766 1104 23 5.109882914102091 1104 24 6.359696972134966 1104 25 5.390713794403894 1105 1 4.194879489745343 1105 2 10.187842882712928 1105 3 7.066445095076009 1105 4 6.2850440415117745 1105 5 2.907811647541955 1105 6 5.4561367396154985 1105 7 6.615477202070025 1105 8 3.60796460421788 1105 9 4.644881830110435 1105 10 4.571059009420683 1105 11 7.873947135785603 1105 12 5.309288625899164 1105 13 6.020002773797394 1105 14 5.281207081648152 1105 15 5.492969821735752 1105 16 5.479765324128124 1105 17 6.192817062428446 1105 18 3.903582781174631 1105 19 3.5101084854431406 1105 20 2.7123415348689797 1105 21 2.8030719645664206 1105 22 4.669378617142268 1105 23 2.2824304867129186 1105 24 5.578504625922324 1105 25 6.160235696254084 1106 1 6.224387686466656 1106 2 10.784564194188338 1106 3 7.581199193436664 1106 4 9.017830926757679 1106 5 8.51600836885343 1106 6 7.972530182238451 1106 7 7.528591956168384 1106 8 7.132554768152307 1106 9 6.272750682569452 1106 10 5.220239337289531 1106 11 7.378865168572489 1106 12 5.140436166882768 1106 13 4.479019167240018 1106 14 5.233666098041592 1106 15 5.604445231571816 1106 16 5.381404668390424 1106 17 6.22396460341205 1106 18 5.9445663877320065 1106 19 5.878960944459532 1106 20 5.1582431742132435 1106 21 5.804711308828715 1106 22 6.277575338453744 1106 23 6.429971470319476 1106 24 7.524406265266588 1106 25 6.0471209421557255 1107 1 3.4088391570939613 1107 2 -13.953140723844449 1107 3 -5.9604778796563025 1107 4 1.2442304803884134 1107 5 3.8087654961483786 1107 6 4.047775286789822 1107 7 4.404883902922908 1107 8 5.0088998541049605 1107 9 2.88793255147505 1107 10 2.363406964890032 1107 11 5.167780331842675 1107 12 2.730018227089481 1107 13 3.9605479819702047 1107 14 3.49800172521698 1107 15 3.3136941090062564 1107 16 3.0204433802962605 1107 17 3.8454381533761826 1107 18 -1.5371298154722837 1107 19 1.3936565897022462 1107 20 2.028711064852775 1107 21 -1.5770699170925866 1107 22 1.9845711138874904 1107 23 -1.5335165866457423 1107 24 2.408233350315875 1107 25 3.20608951032003 1108 1 4.956139472931557 1108 2 10.03884645564273 1108 3 0.22474164824961207 1108 4 5.435101838905638 1108 5 6.864185123792481 1108 6 7.150178353739079 1108 7 7.000297504718194 1108 8 7.077121813129189 1108 9 5.268158416911614 1108 10 4.533811216230266 1108 11 6.831623680552312 1108 12 2.253487869278736 1108 13 3.3639655732929796 1108 14 4.633009309474968 1108 15 4.107991889831588 1108 16 4.239695967255493 1108 17 -0.6768393057934965 1108 18 2.972750498703004 1108 19 3.8551379095709244 1108 20 4.328367175894623 1108 21 4.658688820388193 1108 22 1.6244227533339455 1108 23 4.3178813063850505 1108 24 5.0526796994826455 1108 25 5.629119681121676 1109 1 3.5038958831879654 1109 2 -13.953140723844449 1109 3 -5.668527582519619 1109 4 2.1595249158287153 1109 5 1.3678389699463267 1109 6 2.3688963024570278 1109 7 3.833182120102812 1109 8 4.459685915693292 1109 9 3.643543032296229 1109 10 2.703490703409752 1109 11 0.5751493215779533 1109 12 1.4000263308171945 1109 13 2.773773265600674 1109 14 3.4932016171672764 1109 15 3.7089534282931282 1109 16 3.5940549416144663 1109 17 3.923609412155219 1109 18 3.8983915243440315 1109 19 2.7430866275196415 1109 20 1.7323415348689828 1109 21 0.5274708716702463 1109 22 2.7813458302570204 1109 23 3.198714639718382 1109 24 4.42530790461085 1109 25 4.6216837836857785 1110 1 3.4404938213322964 1110 2 9.16369776412806 1110 3 6.269960389988974 1110 4 7.349868558440399 1110 5 8.068371615994437 1110 6 3.8149615945532496 1110 7 6.553182120102811 1110 8 6.718775262772329 1110 9 5.3255533415745795 1110 10 4.800415102035181 1110 11 2.6811974315435876 1110 12 5.095610523257058 1110 13 10.002364330892771 1110 14 7.1986483525624685 1110 15 6.641513565750174 1110 16 6.1165463505491715 1110 17 6.566238278134598 1110 18 6.777601146337158 1110 19 5.08983920483923 1110 20 5.630210950676538 1110 21 3.3222990503644034 1110 22 5.482273665308568 1110 23 5.857614983360996 1110 24 6.996424743098821 1110 25 7.385034299149698 1111 1 5.343798163318258 1111 2 9.877478168663696 1111 3 7.399984903908177 1111 4 8.262771387055643 1111 5 8.340699990576077 1111 6 7.81564440234845 1111 7 7.486406875803137 1111 8 7.575044221882216 1111 9 6.127164096357035 1111 10 5.375971702323977 1111 11 7.366311102251132 1111 12 6.566003529514266 1111 13 7.873252092962236 1111 14 6.288896514018525 1111 15 5.949588607446223 1111 16 5.718805213058552 1111 17 7.080129499017325 1111 18 6.900400210554668 1111 19 5.61773917909402 1111 20 5.2781395804715885 1111 21 7.976596821724531 1111 22 7.270564071299365 1111 23 7.138931794984398 1111 24 7.205487057705312 1111 25 7.942731557844304 1112 1 -2.865348103066328 1112 2 6.960948623234589 1112 3 5.324943207858389 1112 4 -1.0625025755802184 1112 5 3.432632784379315 1112 6 2.6757863368212895 1112 7 5.3109140788656966 1112 8 5.246266671707037 1112 9 4.174350592433687 1112 10 2.8090061673272793 1112 11 -0.8222389945732509 1112 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5.616700035412258 2204 8 5.685793407549969 2204 9 3.719997972578529 2204 10 3.473691572030816 2204 11 5.099210125052437 2204 12 5.013886265670617 2204 13 2.8520794545257537 2204 14 4.326355797406148 2204 15 4.187373623732867 2204 16 4.546166776576463 2204 17 -0.8954405335559628 2204 18 2.7521917414992956 2204 19 3.6470551400060707 2204 20 3.9361200364976447 2204 21 5.3316456816593885 2204 22 4.708674820485037 2204 23 3.83111420540785 2204 24 5.454672725457755 2204 25 5.791265759798701 2205 1 5.854843750486545 2205 2 10.58556385751416 2205 3 7.160540347970112 2205 4 7.847629181231561 2205 5 7.906330281162756 2205 6 7.440326002299052 2205 7 7.808316401303443 2205 8 7.403422092628521 2205 9 6.062650457256737 2205 10 5.3937197713402405 2205 11 7.691547425843353 2205 12 6.895516062254796 2205 13 9.874499963862917 2205 14 7.369117888889239 2205 15 6.510098767945578 2205 16 6.029023346037846 2205 17 6.740852698095189 2205 18 6.6559744627326545 2205 19 4.7494294395259615 2205 20 5.48927676393691 2205 21 -1.137481734964826 2205 22 3.681993539577718 2205 23 5.051984781898479 2205 24 6.938396372225384 2205 25 7.654867038030172 2206 1 3.896844601136685 2206 2 8.274423378719653 2206 3 5.413844212352174 2206 4 1.492794146597948 2206 5 5.367390251997609 2206 6 5.7527424563031815 2206 7 5.928182120102811 2206 8 5.803083351590725 2206 9 4.354696878450074 2206 10 3.8158624982815468 2206 11 3.7418800908087206 2206 12 4.66534684363771 2206 13 8.26620916303657 2206 14 0.22435546332112466 2206 15 2.3403636847033837 2206 16 3.145144685204208 2206 17 4.177327360873168 2206 18 -1.0993648859123795 2206 19 0.79424047367349 2206 20 2.3139440989715467 2206 21 2.995227281926656 2206 22 -2.034231092819901 2206 23 -3.055516129512384 2206 24 0.8219104687134156 2206 25 2.226876091378088 2207 1 1.1001005036500118 2207 2 -13.953140723844449 2207 3 -6.396910420803447 2207 4 -4.788098846547523 2207 5 1.0566673527846113 2207 6 1.3988137942062053 2207 7 2.5911359154823472 2207 8 2.7518806351652394 2207 9 0.3542526032533253 2207 10 0.196114465785989 2207 11 -1.8575899523494392 2207 12 -2.346854857301615 2207 13 0.17199108738284963 2207 14 -5.475758778872326 2207 15 -1.5274327103207348 2207 16 -0.3911430781875147 2207 17 0.7459361448284874 2207 18 0.652681953386935 2207 19 0.13373019187607937 2207 20 0.2149817988953835 2207 21 -3.6318195573726584 2207 22 -4.428192123538356 2207 23 -1.3873579675423429 2207 24 0.6478656603864259 2207 25 1.0687299883062416 2208 1 5.281602889557394 2208 2 6.3414452634166985 2208 3 6.322130591578045 2208 4 8.390076932813855 2208 5 4.400758290540807 2208 6 6.57209163154408 2208 7 6.8541375341155515 2208 8 6.694813304228322 2208 9 4.936356195778181 2208 10 4.572609599375781 2208 11 7.037803249391114 2208 12 6.488911681135669 2208 13 9.178486641396852 2208 14 3.802299281710802 2208 15 5.007520307274021 2208 16 5.583216300425505 2208 17 6.415976715764565 2208 18 1.814739719673117 2208 19 4.362630151935779 2208 20 5.0766727450600655 2208 21 7.590411423687232 2208 22 6.882874492677406 2208 23 2.648693408295877 2208 24 4.073492617986645 2208 25 5.6172994949384325 2209 1 2.2975074675150537 2209 2 4.017898458438346 2209 3 4.244362820660392 2209 4 0.6187469487645458 2209 5 4.7015726439951635 2209 6 4.90930232176424 2209 7 5.593216191653067 2209 8 4.143697840735882 2209 9 3.8093664280940693 2209 10 2.781034712495501 2209 11 -0.6636013882460112 2209 12 2.4301029918052706 2209 13 1.5022911531645562 2209 14 2.8082129743506954 2209 15 3.3535445696900616 2209 16 2.638742619070456 2209 17 0.8982573394692466 2209 18 2.732775397143577 2209 19 2.9794608467132804 2209 20 3.288491449690106 2209 21 5.313072802391424 2209 22 4.045894382216137 2209 23 -0.3804790002589016 2209 24 -4.685228722305673 2209 25 -1.3084212702608404 2210 1 -1.636121629946885 2210 2 -13.953140723844449 2210 3 -7.384931552163518 2210 4 -1.5563127957883296 2210 5 0.25766091974200833 2210 6 -0.3476156648517872 2210 7 -0.20250964872380806 2210 8 -21.19531408430671 2210 9 -12.703511842192972 2210 10 -6.359956465300112 2210 11 -9.572264572175698 2210 12 -3.6707004642791254 2210 13 -3.9878379428056334 2210 14 -3.1820727552787247 2210 15 -4.9523863265229835 2210 16 -4.428750078817526 2210 17 -3.944040908918918 2210 18 -4.062174733682824 2210 19 -9.669981089935117 2210 20 -6.528288937985661 2210 21 -4.482053354832967 2210 22 -9.412051717904099 2210 23 -7.53139627680234 2210 24 -10.953334827438187 2210 25 -8.266929759805173 2211 1 -0.025965227923144596 2211 2 4.831581498377773 2211 3 0.6033474174803821 2211 4 1.3800804713842716 2211 5 1.2190195255018814 2211 6 0.1214657469014746 2211 7 -20.94681787989719 2211 8 -13.33941130652893 2211 9 -8.944581967703773 2211 10 -7.225537074368024 2211 11 -9.923184011755382 2211 12 -4.534973669182804 2211 13 -7.028796178843772 2211 14 -4.590548382832722 2211 15 -5.065352127262429 2211 16 -12.041153391718867 2211 17 -7.766182254511445 2211 18 -6.076261253433746 2211 19 -9.261982816924803 2211 20 -6.553977909575462 2211 21 -4.36120968388531 2211 22 -5.878515280854089 2211 23 -7.9946186936149495 2211 24 -22.42969209538915 2211 25 -22.226649549647554 2212 1 -1.8529141526543285 2212 2 -13.953140723844449 2212 3 -8.202936184670156 2212 4 -19.883808417504618 2212 5 -20.39382769672034 2212 6 -21.109437030876304 2212 7 -10.371549062692887 2212 8 -10.08957931728162 2212 9 -5.791636179173306 2212 10 -6.324663418454048 2212 11 -6.728291538637102 2212 12 -3.707016679935492 2212 13 -6.950671178843772 2212 14 -4.6173180960943725 2212 15 -4.386476679233752 2212 16 -4.812378750141807 2212 17 -4.849347577092093 2212 18 -3.946948977448084 2212 19 -4.545640971046666 2212 20 -4.76471939703066 2212 21 -2.4963463326308286 2212 22 -3.822847718130074 2212 23 -3.82078356816692 2212 24 -2.9046024896543834 2212 25 -3.151380732443254 2213 1 -18.946104116812034 2213 2 -13.953140723844449 2213 3 -8.431420664280626 2213 4 -5.52531785146688 2213 5 -20.39382769672034 2213 6 -10.683021936536681 2213 7 -20.94681787989719 2213 8 -10.546257480533123 2213 9 -8.280450678395596 2213 10 -7.122012441244337 2213 11 -6.517051936283682 2213 12 -4.7595019710695965 2213 13 -19.506226734399327 2213 14 -12.300729200442786 2213 15 -6.662272986801213 2213 16 -6.009687196750313 2213 17 -4.42453524193283 2213 18 -4.539658790121379 2213 19 -5.247825322165891 2213 20 -5.429167899093283 2213 21 -3.6985983107196923 2213 22 -4.542993792384486 2213 23 -4.173863976633818 2213 24 -3.0712015293514128 2213 25 -3.8908004930437805 2214 1 -18.946104116812034 2214 2 -13.953140723844449 2214 3 -12.700933007216264 2214 4 -19.883808417504618 2214 5 -20.39382769672034 2214 6 -21.109437030876304 2214 7 -16.89328468931903 2214 8 -15.383750915141826 2214 9 -14.702199294613122 2214 10 -14.47227303677583 2214 11 -15.256428123104419 2214 12 -12.970733840489014 2214 13 -19.506226734399327 2214 14 -17.254739139435152 2214 15 -24.16604657170687 2214 16 -18.761765900641066 2214 17 -23.854723921178113 2214 18 -18.33119448564883 2214 19 -15.824040424585997 2214 20 -14.20157709468134 2214 21 -11.890626915624544 2214 22 -12.98548500486075 2214 23 -12.918601562993965 2214 24 -11.680227427294932 2214 25 -11.500739485407726 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 57382.0 3 0.0 4 236.0 5 1046.0 6 1426.0 7 1339.0 8 1178.0 9 1158.0 10 1048.0 11 978.0 12 980.0 13 1021.0 14 1624.0 15 3394.0 16 4905.0 17 5369.0 18 5535.0 19 6352.0 20 7713.0 21 8837.0 22 9382.0 23 10032.0 24 10653.0 25 11149.0 26 9237.0 27 7153.0 28 7536.0 29 8825.0 30 8591.0 31 10005.0 32 13096.0 33 16546.0 34 20207.0 35 23434.0 36 23193.0 37 24350.0 38 18401.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.20727632387928 18.45077387794381 11.494894276778316 25.847055521398595 2 14.677868345402278 19.848713656708274 40.99150322040683 24.481914777482626 3 19.806470097134635 22.992535451215492 28.511618879793964 28.68937557185591 4 11.335847944205117 16.593081916910645 38.6748715337563 33.39619860512793 5 12.202774884939698 38.464742836026474 36.31135149662378 13.02113078241005 6 31.771541767437444 37.67430658964557 18.128224523508283 12.425927119408705 7 28.14367035052181 31.022269801789786 21.986140670273596 18.847919177414806 8 24.548952436129145 37.99473541691594 20.048867421928627 17.40744472502629 9 25.592353493471425 15.883762836998546 19.909084017098504 38.61479965243152 10 13.286760796360845 30.43244520588409 35.289182962131505 20.99161103562356 11 33.89144927357642 22.706744217569717 24.03574817315868 19.366058335695186 12 22.27084172557895 25.336945458347298 32.100762297778914 20.29145051829484 13 27.93413060050292 21.278547948135092 25.452300220415513 25.335021230946474 14 19.50535554234295 22.424651172421544 28.204081511916492 29.865911773319016 15 22.169924451373067 31.34878282767718 23.67160330974937 22.809689411200385 16 21.397717864727692 29.531574337376863 27.406620831983492 21.664086965911956 17 20.966509327938862 29.021652805873977 28.542743687720083 21.46909417846707 18 20.726448359742385 28.602167699678716 30.093015419810776 20.578368520768123 19 21.91771514326144 27.931770982579383 27.779927741523807 22.370586132635367 20 22.004843548702787 28.368939356316332 29.090213285579487 20.536003809401393 21 21.656684199325042 26.99796296859323 27.47955367729774 23.865799154783986 22 21.281896510912276 28.698870899020022 28.733489561141884 21.285743028925815 23 21.455356622149647 28.122995276141598 28.475826675220155 21.9458214264886 24 21.243806715136085 27.837396085556094 29.313868799918115 21.60492839938971 25 21.746927872810726 28.031155773032935 28.663369512600532 21.558546841555806 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 328.0 1 328.0 2 742.0 3 1156.0 4 1156.0 5 1156.0 6 1608.5 7 2061.0 8 2061.0 9 2061.0 10 2461.0 11 2861.0 12 2861.0 13 2861.0 14 3596.5 15 4332.0 16 4332.0 17 4332.0 18 5999.0 19 7666.0 20 7666.0 21 7666.0 22 10542.5 23 13419.0 24 13419.0 25 13419.0 26 17387.5 27 21356.0 28 21356.0 29 21356.0 30 26012.5 31 30669.0 32 30669.0 33 30669.0 34 33927.5 35 37186.0 36 37186.0 37 37186.0 38 39595.5 39 42005.0 40 42005.0 41 42005.0 42 42778.0 43 43551.0 44 43551.0 45 43551.0 46 42651.0 47 41751.0 48 41751.0 49 41751.0 50 38557.0 51 35363.0 52 35363.0 53 35363.0 54 30703.0 55 26043.0 56 26043.0 57 26043.0 58 21335.0 59 16627.0 60 16627.0 61 16627.0 62 13009.0 63 9391.0 64 9391.0 65 9391.0 66 7021.0 67 4651.0 68 4651.0 69 4651.0 70 3289.0 71 1927.0 72 1927.0 73 1927.0 74 1304.0 75 681.0 76 681.0 77 681.0 78 444.5 79 208.0 80 208.0 81 208.0 82 136.0 83 64.0 84 64.0 85 64.0 86 37.5 87 11.0 88 11.0 89 11.0 90 7.0 91 3.0 92 3.0 93 3.0 94 2.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.827727628884597 2 35.962436391493426 3 5.449869069153042 4 13.046188441384052 5 13.29435992438343 6 14.12713254163135 7 9.12263224889386 8 7.490875620064606 9 0.10398734674974003 10 1.389702048579859 11 5.448995225903045 12 5.275100419153479 13 15.555574974294444 14 1.529225687496177 15 2.8402818435762325 16 2.8950426872427624 17 2.806784518992983 18 1.4505797949963735 19 1.399314324329835 20 0.29128108333260516 21 4.194738881072847 22 1.5563148282461092 23 0.1080652819163965 24 8.93475595014433 25 0.11330834141638339 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 343311.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.7898516459642 #Duplication Level Percentage of deduplicated Percentage of total 1 98.48627814023851 95.32472250356854 2 1.1494645721554257 2.225130108224308 3 0.1798276630962986 0.5221647849879353 4 0.05512804613547137 0.21343341627936577 5 0.03829710080792272 0.1853385352834688 6 0.019938637304618038 0.11579146480420004 7 0.013218051568352705 0.08955612752446938 8 0.009329646408760708 0.07224120734506013 9 0.008212224095105868 0.07153739566638385 >10 0.03545929078967764 0.6731585714114549 >50 0.0024233137007801603 0.16360014105501808 >100 0.002423313699066613 0.34332574384980485 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 45 1.9374693E-6 23.619112 1 GCCCTGA 30 0.0024778962 22.142918 1 GTGTAGG 65 9.955511E-8 20.439615 1 TATTAGT 35 0.005246252 19.454012 2 GTCTAGG 45 0.0012310493 17.714334 1 TGTAGGA 175 0.0 16.341368 2 TAGGACC 55 7.189681E-5 15.53634 4 CACTGAC 40 0.0033367323 15.261427 7 AGACTGA 55 9.038478E-5 15.125538 6 TAGGACT 50 6.155466E-4 14.953727 4 TCCACGT 40 0.004442771 14.618781 8 GTCCTAC 205 0.0 14.257878 1 TTCCACG 50 8.8994676E-4 14.2439995 7 ACTGACC 55 1.5703023E-4 14.175789 8 TTAGGAC 55 0.0010850414 13.874797 3 AATACTC 45 0.0062855333 13.87335 5 CTATACT 65 2.973918E-4 13.146132 4 TAGGACA 90 1.9929466E-6 13.054841 4 CTTAATC 60 0.002090183 12.718564 3 GTAGGAC 180 0.0 12.718563 3 >>END_MODULE