FastQCFastQC Report
Thu 26 May 2016
SRR1926444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926444_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343311
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT5090.14826207141629602No Hit
CACACACACACACACACACACACAC4290.12495958474968762No Hit
GGTATCAACGCAGAGTACTTTTTTT4280.124668303666355No Hit
CTGTAGGACGTGGAATATGGCAAGA4090.1191339630830355No Hit
GTCCTACAGTGTGCATTTCTCATTT4050.11796883874970508No Hit
GTCCTACAGTGGACATTTCTAAATT3970.11563859008304424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG250.00612614818.94406518
AGCACCG350.002111605116.3473075
TAGGACC1300.015.4041924
CCTCTAT508.4704225E-515.2440741
TCAGGGG456.5383536E-414.8380193
GTAGGAC2250.014.4140763
CTAAGAC400.005127632514.308093
ATAAACT400.005127632514.308093
CCTACAC602.460446E-514.3080893
TAGGACT602.4672669E-514.3038934
GTAGACC706.9255893E-613.6267523
CTAGGAC500.001450210713.3542183
TCTATAC500.001450210713.3542183
CTTGGAC655.224863E-513.2074683
AGGACCT2100.013.1686645
GACAGAT655.3853466E-513.1649817
GGACCTG2200.013.003546
GGCGAGA1900.012.96172819
TGTAGGA2650.012.9601712
CTGTAGG2500.012.9574611
Produced by FastQC (version 0.11.5)