##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926444_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 343311 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.405795328433985 31.0 28.0 31.0 22.0 34.0 2 28.89221143511277 31.0 28.0 33.0 23.0 34.0 3 29.259170256706017 31.0 29.0 33.0 25.0 34.0 4 33.01505340638663 35.0 33.0 37.0 27.0 37.0 5 32.67760427134581 35.0 33.0 37.0 26.0 37.0 6 32.791163114493855 35.0 33.0 37.0 26.0 37.0 7 32.62385999866011 35.0 33.0 37.0 25.0 37.0 8 32.57826577068605 35.0 33.0 37.0 25.0 37.0 9 33.819766916877114 37.0 34.0 39.0 23.0 39.0 10 33.73244667371567 37.0 33.0 39.0 23.0 39.0 11 33.973464293308396 37.0 34.0 39.0 25.0 39.0 12 33.74933806373813 37.0 34.0 39.0 24.0 39.0 13 33.9218609365852 37.0 34.0 39.0 25.0 39.0 14 34.545837447678636 38.0 34.0 40.0 22.0 41.0 15 34.68324056030829 38.0 34.0 40.0 24.0 41.0 16 34.662871274150845 38.0 34.0 40.0 24.0 41.0 17 34.58504970711687 38.0 34.0 40.0 24.0 41.0 18 34.556807093276944 38.0 34.0 40.0 23.0 41.0 19 34.59331043864018 39.0 34.0 40.0 22.0 41.0 20 34.57825411944272 39.0 34.0 40.0 22.0 41.0 21 34.49162421244877 39.0 34.0 40.0 20.0 41.0 22 34.43767604300474 39.0 34.0 40.0 19.0 41.0 23 34.09861029795142 39.0 34.0 40.0 18.0 41.0 24 34.163935906510424 39.0 34.0 40.0 18.0 41.0 25 33.96067122812843 39.0 34.0 40.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7561093163565857 1101 2 0.849143963832514 1101 3 0.7982653328402627 1101 4 0.74220467397177 1101 5 0.6146385382750879 1101 6 0.5536630613976925 1101 7 0.5888252836475729 1101 8 0.6311314515168363 1101 9 1.1212028152072833 1101 10 0.8778896277059687 1101 11 0.7055489491133713 1101 12 0.7393345610440107 1101 13 0.35007249789043726 1101 14 0.9338206486100447 1101 15 0.6070863702907445 1101 16 0.6765933115470446 1101 17 0.5263378602213038 1101 18 0.5914695780051602 1101 19 0.6001337306275119 1101 20 0.635375152024956 1101 21 0.882540812430328 1101 22 0.4082005345989188 1101 23 0.828170789782753 1101 24 0.8788662932535161 1101 25 0.9414226914792607 1102 1 0.6486516892379406 1102 2 0.9047089355839262 1102 3 0.5911184401848963 1102 4 0.2931368773616043 1102 5 0.8041583122863898 1102 6 0.420668711115205 1102 7 0.6960851706532267 1102 8 0.8146060277880238 1102 9 1.1007225892185843 1102 10 1.0453755034121812 1102 11 0.7985715479834283 1102 12 0.974165069518591 1102 13 0.8835470741616263 1102 14 1.2824364678190818 1102 15 1.010363206448936 1102 16 0.9308023510950676 1102 17 1.127383057961417 1102 18 1.2393791825249352 1102 19 1.1315744085936146 1102 20 1.023284756544733 1102 21 1.281862846328636 1102 22 1.1445564668023138 1102 23 1.3570408462799293 1102 24 1.1771713779992794 1102 25 1.271337945716546 1103 1 0.1387714323975402 1103 2 0.33463315609644795 1103 3 0.1731743203260443 1103 4 -0.1709864523080924 1103 5 -0.09535577344277613 1103 6 -0.32526754156021553 1103 7 -0.26476402238200336 1103 8 -0.13891974302241294 1103 9 0.3329491178238939 1103 10 0.20680316581745473 1103 11 -0.28380827500494377 1103 12 -0.3221500805942128 1103 13 -0.17134388436211623 1103 14 -0.07615090997926188 1103 15 -0.22617870365692738 1103 16 0.06358421029562322 1103 17 0.030541500721874115 1103 18 0.14330120485385578 1103 19 -0.18012224206873384 1103 20 0.07089278569958424 1103 21 -0.2921349554877608 1103 22 -0.07741379987206898 1103 23 -0.04512841385774635 1103 24 -0.31464906510825585 1103 25 0.029665012298373483 1104 1 0.3115433999578734 1104 2 0.4296530742290834 1104 3 0.3167165654233308 1104 4 0.08222075114218796 1104 5 0.18345418672096514 1104 6 -0.17748377675879112 1104 7 0.16949516574832302 1104 8 0.43699961871941184 1104 9 0.9870924186370829 1104 10 0.7901136362804593 1104 11 0.29084905629451185 1104 12 0.4743667153848534 1104 13 0.3567445236139122 1104 14 0.6153265435725288 1104 15 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1107 14 1.2267514968002118 1107 15 1.0053251601340705 1107 16 1.108729994421168 1107 17 0.8726128467150929 1107 18 1.026715391079179 1107 19 1.0686345410002858 1107 20 1.1694540283080457 1107 21 1.3113576681839731 1107 22 0.85282776312404 1107 23 1.2857774888643263 1107 24 1.0777317713734504 1107 25 1.066211994558678 1108 1 1.0095708548181257 1108 2 0.9224131946017451 1108 3 0.7750602046351354 1108 4 0.28560210986920964 1108 5 -0.649399923263374 1108 6 0.24039383062846298 1108 7 0.853120155442447 1108 8 0.7481186310040187 1108 9 1.1115874305919036 1108 10 1.314492191808533 1108 11 0.7245874106518357 1108 12 0.9147832789927293 1108 13 0.7584058312237758 1108 14 1.2545898793792745 1108 15 0.8272145754189495 1108 16 1.0311445935983272 1108 17 1.343517347400791 1108 18 0.821213167748752 1108 19 1.468210653704432 1108 20 1.2650546392044433 1108 21 1.148181838071359 1108 22 0.8539056628040527 1108 23 1.380606687218652 1108 24 1.0798278317150576 1108 25 1.0177688453254135 1109 1 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1.0488241270047993 2205 16 0.8210719845328285 2205 17 0.9801530260980797 2205 18 0.9897634168677243 2205 19 1.132811455745994 2205 20 1.0968917397026843 2205 21 1.091735125226542 2205 22 0.803800850554687 2205 23 1.026417235280384 2205 24 1.0862912537590432 2205 25 1.3407196898994798 2206 1 -0.06574965800238886 2206 2 0.31343883562738384 2206 3 0.43371405078898206 2206 4 0.02758928935638494 2206 5 0.28329238442893256 2206 6 -0.14422155398692382 2206 7 0.2569663092886003 2206 8 -0.07720188181649945 2206 9 0.28145922546369917 2206 10 0.34494090975724845 2206 11 -0.23534848678406206 2206 12 -0.05476800305854823 2206 13 -0.2592223739044286 2206 14 0.08952577681517226 2206 15 -0.11188798868361971 2206 16 0.2539010038547431 2206 17 -0.0042390628556177035 2206 18 -0.33583811430253263 2206 19 -0.19525088475710106 2206 20 -0.025009463359658923 2206 21 0.003951068840585492 2206 22 -0.12686356796518083 2206 23 0.11297848209044759 2206 24 0.11027654966377298 2206 25 0.524179101735669 2207 1 -1.143164611036152 2207 2 0.04539808924505451 2207 3 0.3677867849854799 2207 4 0.0636073142357958 2207 5 0.052443818803141085 2207 6 0.19867956304786105 2207 7 0.3383797390931207 2207 8 0.446725510922775 2207 9 0.5985130462303871 2207 10 0.43747708645184247 2207 11 -0.019748080589593542 2207 12 -0.07441131354344321 2207 13 -0.486643673726725 2207 14 -5.5401232457793945 2207 15 -2.764464784824767 2207 16 -1.224710318815987 2207 17 -1.0458898625509718 2207 18 -0.6035139203446747 2207 19 -0.5885296357091221 2207 20 -0.38858524401464933 2207 21 0.20504906325541583 2207 22 -0.010066792133748947 2207 23 -0.03786881417764221 2207 24 -0.10859245262106754 2207 25 -0.1420591567055567 2208 1 0.8906953036177327 2208 2 1.1962883375055497 2208 3 1.0253460122457838 2208 4 0.7680963937169949 2208 5 1.1020801518631984 2208 6 1.0904571165993957 2208 7 1.153167109549912 2208 8 1.2354308145741584 2208 9 1.3454597154196009 2208 10 1.1446306043513985 2208 11 1.2000181635507374 2208 12 1.121595707540827 2208 13 1.1320788673171904 2208 14 1.5973344490346761 2208 15 1.4217148203969003 2208 16 1.5196612520990627 2208 17 1.0710406203062277 2208 18 1.6874143763066485 2208 19 1.1390827752135024 2208 20 1.2031670840844058 2208 21 1.1018401754876521 2208 22 1.088359770267708 2208 23 1.4834361825640698 2208 24 1.4963822168203933 2208 25 1.443938615046136 2209 1 -0.2948617228256971 2209 2 -0.13242616677509744 2209 3 0.48603080700267043 2209 4 0.529538575164274 2209 5 0.7741984474176178 2209 6 0.4814200176725407 2209 7 0.6955856470774435 2209 8 0.8400326440210932 2209 9 0.5765548878932591 2209 10 0.5750985998808673 2209 11 0.3608272001071242 2209 12 0.46440270414452556 2209 13 0.6588885115190593 2209 14 0.643851880864446 2209 15 0.8958654730732505 2209 16 0.9433366392017888 2209 17 1.0910766450026799 2209 18 1.0486189249671156 2209 19 1.1822717204060496 2209 20 0.9013490305030913 2209 21 0.9768849350879094 2209 22 1.1572380133042017 2209 23 1.2752633508276148 2209 24 1.055527281328473 2209 25 1.2769337647330943 2210 1 -0.030860911314167794 2210 2 -0.7153539929684207 2210 3 0.3645904933773352 2210 4 0.4925461801013711 2210 5 0.6219385966522069 2210 6 0.72572377359851 2210 7 0.9123308294736105 2210 8 0.8108599979266415 2210 9 1.2997638192936805 2210 10 1.0634733988676714 2210 11 1.090945913503333 2210 12 0.8274168727778175 2210 13 0.9649148796767832 2210 14 1.2292351261348529 2210 15 0.921633947640423 2210 16 1.1945471936252758 2210 17 1.1303454492464056 2210 18 0.9395839971528588 2210 19 1.4593193698569351 2210 20 1.688813564167397 2210 21 1.6400539472814657 2210 22 1.3146891510612662 2210 23 1.4048724827191208 2210 24 1.3273776767911727 2210 25 1.4233578928803112 2211 1 -0.8986823503100823 2211 2 -0.15731436950082056 2211 3 0.3997931106180417 2211 4 0.47296856286065747 2211 5 0.7791524271639787 2211 6 0.6812325058421393 2211 7 0.8320197280920212 2211 8 0.6589092292946148 2211 9 1.1489805929850618 2211 10 1.430945183261521 2211 11 1.0199933935578187 2211 12 1.283640116599571 2211 13 0.9580586090015544 2211 14 1.266876204165598 2211 15 1.0161835925129665 2211 16 1.0997877559914926 2211 17 1.2677961935546378 2211 18 1.4016084668940536 2211 19 1.4131892861830693 2211 20 1.22058348535829 2211 21 1.3030963679858871 2211 22 1.1250060901544785 2211 23 1.7487957897827542 2211 24 1.662894071031296 2211 25 1.7250338025903744 2212 1 0.7233494597257604 2212 2 0.14930525415509166 2212 3 0.09950189198005077 2212 4 0.3096061076635266 2212 5 0.25053459562276004 2212 6 0.24513259544787047 2212 7 0.5787715202067147 2212 8 0.44161532248458 2212 9 0.7488909872502987 2212 10 0.2223878355912703 2212 11 0.584957551263912 2212 12 0.6728650269938328 2212 13 0.5352875516538802 2212 14 0.6371719030903336 2212 15 0.5750433595380571 2212 16 -0.3783708461590436 2212 17 0.15606904301700553 2212 18 0.29777782173275824 2212 19 -0.015798169014061614 2212 20 0.19028554629019112 2212 21 0.4469135722869595 2212 22 0.30119336614014003 2212 23 0.591145700177016 2212 24 0.46811360508147715 2212 25 -0.04938376013364376 2213 1 0.29988290126224726 2213 2 0.15760308332936646 2213 3 -0.4102566797383531 2213 4 -1.068455703386718 2213 5 -2.002877184995352 2213 6 -0.6279721587280918 2213 7 -0.6508288044027388 2213 8 -0.5108496805586356 2213 9 -1.0346147948556066 2213 10 -0.6924562842437183 2213 11 -0.7507089125218442 2213 12 -0.9024578917861774 2213 13 -0.602285992675597 2213 14 -8.298663628119513 2213 15 -3.769265830967118 2213 16 -2.2330607765032653 2213 17 -1.5428759762566813 2213 18 -5.4219895414916905 2213 19 -3.2227907976743744 2213 20 -5.872046231622843 2213 21 -2.351044093230044 2213 22 -1.9122711635142835 2213 23 -4.616671977512844 2213 24 -2.1550959708974275 2213 25 -1.968860327388665 2214 1 0.8016767681767156 2214 2 0.4813031358524995 2214 3 -0.8181836321847165 2214 4 -1.3054077457284414 2214 5 -0.15525082646438193 2214 6 -0.6562013211861739 2214 7 -0.7300862081440016 2214 8 -0.20050666411485452 2214 9 -5.0815987550996375 2214 10 -1.0042053044853247 2214 11 -0.5294653264041145 2214 12 -0.8297982012686234 2214 13 -1.3795313564992782 2214 14 -2.4430779952157913 2214 15 -1.4706152713937684 2214 16 -0.932874925426546 2214 17 -1.8314744167237365 2214 18 -1.0947259965844154 2214 19 -4.2086135927129575 2214 20 -2.1964735274896725 2214 21 -4.056260044100718 2214 22 -5.306820878677311 2214 23 -4.863813507148013 2214 24 -5.183874606104084 2214 25 -2.9970248459939732 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10856.0 3 9.0 4 202.0 5 1282.0 6 1634.0 7 1305.0 8 1011.0 9 819.0 10 705.0 11 688.0 12 645.0 13 642.0 14 612.0 15 665.0 16 663.0 17 744.0 18 772.0 19 980.0 20 1214.0 21 1623.0 22 2052.0 23 2517.0 24 3076.0 25 3521.0 26 4117.0 27 4620.0 28 5243.0 29 6538.0 30 7958.0 31 10178.0 32 12912.0 33 16683.0 34 21640.0 35 28746.0 36 38485.0 37 56686.0 38 91268.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.651143469325284 19.21553194280467 8.679580800792921 30.45374378707712 2 13.766517577470536 21.12996984221798 38.99066128585789 26.11285129445359 3 19.17285346324635 23.043146350275638 28.983927926247944 28.80007226023007 4 11.928099814101481 16.796426552602288 37.431162186259826 33.844311447036404 5 12.873413185167488 38.41046095932917 35.48783721180937 13.228288643693967 6 32.393365552446696 38.21619551329842 16.907432908520523 12.483006025734358 7 29.113539769277473 31.608752510391852 20.25594320676288 19.021764513567792 8 25.324906585707613 38.60695936478281 19.092713685193836 16.97542036431574 9 25.59195910658037 15.839485737806855 19.511077247342715 39.05747790827006 10 13.913116941380737 30.651156828814972 34.18922934400727 21.24649688579702 11 35.46304846184544 23.146742945694886 22.668445474962205 18.721763117497474 12 22.298799013110944 25.75902779193769 31.50791870690735 20.434254488044022 13 29.120535025093865 21.721995508445698 25.408740180186477 23.748729286273964 14 19.959950681625408 22.589098074193256 27.431742728308805 30.01920851587253 15 22.632842220552167 31.397137214464234 22.79654648094098 23.173474084042624 16 21.66615691586199 29.857415417777194 27.024948660811816 21.451479005549 17 21.16027136694252 29.542177518722745 27.43847200254006 21.859079111794674 18 20.869149813137742 29.10361461224989 29.27838835307791 20.748847221534454 19 22.743849510349367 27.86115011097971 27.361887062853548 22.033113315817378 20 22.341745604530043 28.433651415055866 28.37277313665863 20.85182984375546 21 22.06169351315138 27.617605079956892 27.653432757566048 22.667268649325685 22 21.777921476445556 28.857508206844525 27.880260172263633 21.48431014444629 23 21.808214709112146 28.227467223596097 27.88375554526362 22.08056252202813 24 21.833683358140917 27.992857746228616 28.665429685793264 21.5080292098372 25 21.786951190028866 28.29154906192927 28.180862250262884 21.74063749777898 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 69.0 1 69.0 2 87.5 3 106.0 4 106.0 5 106.0 6 156.5 7 207.0 8 207.0 9 207.0 10 394.0 11 581.0 12 581.0 13 581.0 14 992.0 15 1403.0 16 1403.0 17 1403.0 18 2441.0 19 3479.0 20 3479.0 21 3479.0 22 5612.5 23 7746.0 24 7746.0 25 7746.0 26 11084.0 27 14422.0 28 14422.0 29 14422.0 30 19012.5 31 23603.0 32 23603.0 33 23603.0 34 28115.0 35 32627.0 36 32627.0 37 32627.0 38 36553.5 39 40480.0 40 40480.0 41 40480.0 42 42957.5 43 45435.0 44 45435.0 45 45435.0 46 46643.0 47 47851.0 48 47851.0 49 47851.0 50 46044.5 51 44238.0 52 44238.0 53 44238.0 54 38620.0 55 33002.0 56 33002.0 57 33002.0 58 28000.0 59 22998.0 60 22998.0 61 22998.0 62 18305.0 63 13612.0 64 13612.0 65 13612.0 66 10219.0 67 6826.0 68 6826.0 69 6826.0 70 4904.5 71 2983.0 72 2983.0 73 2983.0 74 2063.0 75 1143.0 76 1143.0 77 1143.0 78 745.0 79 347.0 80 347.0 81 347.0 82 231.0 83 115.0 84 115.0 85 115.0 86 71.5 87 28.0 88 28.0 89 28.0 90 18.5 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08039357899979901 2 0.0337886056665822 3 0.03116707591658875 4 0.03291476241658438 5 0.02679785966659967 6 0.37225722449906934 7 0.21409159624946475 8 0.21875209358278644 9 0.22195618549944512 10 0.013107648749967232 11 0.0029128108333260514 12 0.0029128108333260514 13 0.0 14 0.0681597734998296 15 0.0026215297499934463 16 0.0017476864999956307 17 0.0034953729999912613 18 0.0029128108333260514 19 0.0014564054166630257 20 8.738432499978153E-4 21 2.9128108333260514E-4 22 0.0 23 0.0 24 5.825621666652103E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 343311.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.80553900240844 #Duplication Level Percentage of deduplicated Percentage of total 1 95.13536212074251 86.38817835563334 2 3.9372358481738075 7.150456467460548 3 0.46080822572787167 1.2553181794188863 4 0.15182782244189277 0.5514722900959216 5 0.08763362840874575 0.39788094311964645 6 0.05077027381805062 0.2766133247608771 7 0.03676871840056637 0.23371623049538415 8 0.019620894720311643 0.14253487366299272 9 0.01729490193056566 0.14134256026189285 >10 0.0879365539457337 1.4671511853436532 >50 0.008652332951089423 0.5485208674679206 >100 0.005768221963105323 1.2986995385218978 >500 3.204567757280735E-4 0.14811518375703472 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 509 0.14826207141629602 No Hit CACACACACACACACACACACACAC 429 0.12495958474968762 No Hit GGTATCAACGCAGAGTACTTTTTTT 428 0.124668303666355 No Hit CTGTAGGACGTGGAATATGGCAAGA 409 0.1191339630830355 No Hit GTCCTACAGTGTGCATTTCTCATTT 405 0.11796883874970508 No Hit GTCCTACAGTGGACATTTCTAAATT 397 0.11563859008304424 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 2.9128108333260514E-4 0.0 0.0 0.0 13 0.0 2.9128108333260514E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 25 0.006126148 18.944065 18 AGCACCG 35 0.0021116051 16.347307 5 TAGGACC 130 0.0 15.404192 4 CCTCTAT 50 8.4704225E-5 15.244074 1 TCAGGGG 45 6.5383536E-4 14.838019 3 GTAGGAC 225 0.0 14.414076 3 CTAAGAC 40 0.0051276325 14.30809 3 ATAAACT 40 0.0051276325 14.30809 3 CCTACAC 60 2.460446E-5 14.308089 3 TAGGACT 60 2.4672669E-5 14.303893 4 GTAGACC 70 6.9255893E-6 13.626752 3 CTAGGAC 50 0.0014502107 13.354218 3 TCTATAC 50 0.0014502107 13.354218 3 CTTGGAC 65 5.224863E-5 13.207468 3 AGGACCT 210 0.0 13.168664 5 GACAGAT 65 5.3853466E-5 13.164981 7 GGACCTG 220 0.0 13.00354 6 GGCGAGA 190 0.0 12.961728 19 TGTAGGA 265 0.0 12.960171 2 CTGTAGG 250 0.0 12.957461 1 >>END_MODULE