FastQCFastQC Report
Thu 26 May 2016
SRR1926443_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926443_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775863
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATCC300.007636574618.236513
TAGGACC1550.017.2869854
GGTCTAC951.654189E-816.8342231
GTCCTAC4550.016.6954781
TGTAGGA4050.016.1963162
TCCTACA5150.015.3904322
CTGTAGG4000.014.6598021
CCTGTAC1351.8189894E-1214.5892083
TGTAAGA852.216024E-514.4695032
AGGACCT2600.014.4395525
CCTACAG5600.014.2635563
CTATTAG751.5369135E-414.2155661
CTCCTAG1059.409305E-713.9617171
GTAGGAC4000.013.950933
TCCTAGG600.00695641613.6656422
GGCTATA600.00695641613.6656422
GACTGAT602.264003E-413.5825337
CCAAGAC901.5296682E-613.3734413
GTTCTAG904.8071204E-513.3270931
GTCTAAG600.0082122613.3270931
Produced by FastQC (version 0.11.5)