FastQCFastQC Report
Thu 26 May 2016
SRR1926443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926443_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775863
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA11980.15440870359844455No Hit
GTCCTACAGTGGACATTTCTAAATT11620.1497687091664379No Hit
GTCCTACAGTGTGCATTTCTCATTT10040.12940428915929747No Hit
CTTTAGGACGTGAAATATGGCGAGG8550.11019986776015869No Hit
CCCATGTACTCTGCGTTGATACCAC8400.10826653674682257No Hit
GTACATGGGAAGCAGTGGTATCAAC8360.10775098180993294No Hit
GTCCTAAAGTGTGTATTTCTCATTT8310.1071065381388209No Hit
CTGTAGGACCTGGAATATGGCGAGA8260.10646209446770885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG456.905923E-414.73532218
TAGGCAG706.963959E-613.6246345
TAGGACC3300.013.5833474
GTCCTAA2950.012.9310171
GGCGAGG2250.012.63027719
TTAGGAC3250.012.3274533
ATAGGAC1103.590867E-812.1406743
GTCTTAC550.002978929312.1375231
TAACACT959.896103E-712.0470454
CCTATAC802.7441516E-511.9238763
ACTAGAC657.7438634E-411.7404323
TTAGACT657.7565265E-411.7381464
GTGCATT4050.011.70450711
TAGGACA2550.011.5942964
GTCCTAC5950.011.3798381
TCCTACA6300.011.3590212
AGGACCT5300.011.3367245
GTAGCCC1104.6988862E-711.2734823
ATTACAC855.0967697E-511.2224723
GGACGTG5450.011.1996986
Produced by FastQC (version 0.11.5)