FastQCFastQC Report
Thu 26 May 2016
SRR1926442_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926442_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665161
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTA557.016621E-515.5864634
GTCTAGG604.5638668E-415.5582231
CTACACT907.406925E-915.4782244
CCAACGA458.951241E-515.14923819
TCTGGAC651.4429894E-515.1419753
TCCTATT550.003880035614.921832
TCCTATA656.910869E-414.7305252
CTAGGAT601.2386456E-414.58116153
GGACGTG2600.014.4259076
GTCCTAT658.376219E-414.3614371
GTGTAGC1059.3315975E-713.9706491
AGGACGT2700.013.8973415
TGTAGGA3250.013.8887812
TTTAGGA1807.2759576E-1213.6783452
GTCCTAC3800.013.335621
GTATTAA600.00817480513.3356191
CTATATG600.00817480513.3356191
GACGTGG1151.875378E-913.2830687
GTAGGAC3800.013.2381613
TAGGACG3100.013.13536554
Produced by FastQC (version 0.11.5)