##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926442_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 665161 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.757073851293146 27.0 10.0 31.0 2.0 31.0 2 16.72746297512933 22.0 2.0 31.0 2.0 31.0 3 20.43268471843659 25.0 15.0 31.0 2.0 31.0 4 22.680369113643163 28.0 7.0 35.0 2.0 37.0 5 23.275928684934925 30.0 7.0 35.0 2.0 37.0 6 24.014337882106737 31.0 8.0 35.0 2.0 37.0 7 23.862878310664637 31.0 10.0 35.0 2.0 37.0 8 24.0956354927604 31.0 12.0 35.0 2.0 37.0 9 26.923881586563255 32.0 17.0 37.0 2.0 39.0 10 27.673414406436937 32.0 25.0 38.0 2.0 39.0 11 24.726815011703934 30.0 10.0 37.0 2.0 39.0 12 25.91750568659317 31.0 17.0 37.0 2.0 39.0 13 22.49497189402265 29.0 2.0 37.0 2.0 39.0 14 25.93577043753317 31.0 17.0 38.0 2.0 40.0 15 27.051542408529663 31.0 18.0 38.0 2.0 40.0 16 27.415494895220856 32.0 21.0 39.0 2.0 40.0 17 26.740942418452075 31.0 17.0 39.0 2.0 40.0 18 26.82390879802033 31.0 17.0 38.0 2.0 40.0 19 27.235780510282474 31.0 22.0 39.0 2.0 40.0 20 27.48397004634968 32.0 24.0 39.0 2.0 40.0 21 25.33035009569112 31.0 10.0 38.0 2.0 40.0 22 26.267129311550136 31.0 16.0 38.0 2.0 40.0 23 26.279057250800935 31.0 10.0 39.0 2.0 40.0 24 25.286629853524186 31.0 2.0 39.0 2.0 40.0 25 25.10105974343054 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.986172359519056 1101 2 10.571269684891728 1101 3 7.329757619642894 1101 4 8.90766494836484 1101 5 8.331908141487716 1101 6 7.861825604943416 1101 7 7.727692081886133 1101 8 6.071619047555188 1101 9 5.99381464249565 1101 10 5.689130920902468 1101 11 8.35482380305373 1101 12 5.930118579903244 1101 13 10.603243110791372 1101 14 7.751971870493552 1101 15 7.124025422261681 1101 16 6.597665116110626 1101 17 2.400502136498666 1101 18 5.028978038342885 1101 19 5.771213015876118 1101 20 3.555826316244392 1101 21 0.4987299561409273 1101 22 4.381450637876959 1101 23 5.7309716105604025 1101 24 7.082826559113148 1101 25 7.589876221451032 1102 1 6.261262850547443 1102 2 11.092610516798217 1102 3 7.286393454537187 1102 4 8.562533134714517 1102 5 8.448416178760038 1102 6 7.273191337715847 1102 7 7.2669850820753865 1102 8 7.515481826050735 1102 9 6.295498841100546 1102 10 5.6348884600829585 1102 11 8.29313119984776 1102 12 5.627029143920524 1102 13 10.147366521330802 1102 14 7.581910579493179 1102 15 6.007877262854414 1102 16 6.3030212610810565 1102 17 7.098841035644941 1102 18 6.9017869186086145 1102 19 5.533473823760946 1102 20 6.012616384509425 1102 21 5.022998986825172 1102 22 6.235465399575226 1102 23 6.65538757274566 1102 24 8.012169793185755 1102 25 7.819190695097991 1103 1 5.494282163934063 1103 2 9.673463855838737 1103 3 6.924116107750063 1103 4 8.206050776728784 1103 5 7.567672169247395 1103 6 7.1212666965165 1103 7 7.0770390583850755 1103 8 6.713262758412348 1103 9 5.7967114933929835 1103 10 5.027669568299732 1103 11 5.9181443889848495 1103 12 4.80904597064033 1103 13 9.141668814107945 1103 14 7.2641186019308 1103 15 6.0703208114768685 1103 16 6.033451013679034 1103 17 3.9383391524980773 1103 18 3.0264390434253663 1103 19 4.7206480707828185 1103 20 5.201881437149982 1103 21 7.221939814930867 1103 22 3.175019116592093 1103 23 5.172436917703891 1103 24 6.546361648921668 1103 25 2.3193841357753087 1104 1 5.909720151791824 1104 2 10.425415286888175 1104 3 7.006509863170702 1104 4 7.631029094161569 1104 5 7.580681709576972 1104 6 7.150754987930203 1104 7 7.08484478258282 1104 8 7.068856860754067 1104 9 5.74293872669741 1104 10 4.817782317649257 1104 11 7.892992611014339 1104 12 4.613902865696705 1104 13 9.135597695287476 1104 14 6.9796433200574235 1104 15 6.262862008354581 1104 16 5.810554222698983 1104 17 6.236691277389664 1104 18 3.730688826599696 1104 19 4.802174523514825 1104 20 3.7066516019374944 1104 21 3.020725591166773 1104 22 4.840240278777696 1104 23 5.222740473644915 1104 24 6.3633607816189794 1104 25 5.224848142713732 1105 1 3.9497874407113756 1105 2 9.80874511559028 1105 3 6.70313202226799 1105 4 6.2562346014962245 1105 5 2.183532658483937 1105 6 5.0997659710204175 1105 7 6.387778869823443 1105 8 3.4680271237211393 1105 9 4.441314199167845 1105 10 4.2457599009802856 1105 11 7.405296859899529 1105 12 4.6425597737738435 1105 13 5.919583950148748 1105 14 4.405896774488866 1105 15 4.805979074761229 1105 16 5.227093737353705 1105 17 5.974595996818369 1105 18 3.4971277296526146 1105 19 3.5248456960646557 1105 20 2.5674049953808655 1105 21 3.7141120152979923 1105 22 4.923989989739159 1105 23 2.585499738405712 1105 24 5.588528723297923 1105 25 6.32307335256132 1106 1 6.6304582410799 1106 2 11.59512437298634 1106 3 8.073232635011294 1106 4 9.340570893741148 1106 5 8.977435204512805 1106 6 8.03640480344463 1106 7 7.989465741907793 1106 8 7.521291188300481 1106 9 6.514444893366523 1106 10 5.681440864990751 1106 11 8.292927195811554 1106 12 5.411326682710538 1106 13 4.406682705651008 1106 14 5.6797491603855335 1106 15 5.938817143815498 1106 16 5.817301543615677 1106 17 6.571896416525593 1106 18 6.405148232423855 1106 19 6.374709693373848 1106 20 5.936532384567922 1106 21 6.085011095451836 1106 22 6.337949276675587 1106 23 6.912970088356722 1106 24 7.586550103505878 1106 25 6.665351178273141 1107 1 3.923651803007271 1107 2 -14.194706135488236 1107 3 -5.835372931721469 1107 4 1.5762379319093078 1107 5 4.406567917390397 1107 6 4.591157368976045 1107 7 4.8032921107943025 1107 8 5.274591279487211 1107 9 3.6534865642547985 1107 10 2.875065030921654 1107 11 5.532003604501792 1107 12 3.2378482708070493 1107 13 4.656451844008778 1107 14 4.201746873480236 1107 15 3.96603710647236 1107 16 3.6237975125832413 1107 17 4.407224761124592 1107 18 -0.5648636363251534 1107 19 2.409115315108135 1107 20 2.559631575466618 1107 21 -0.7393900393163619 1107 22 2.246045355646192 1107 23 -0.5976575079321229 1107 24 2.8594401438885697 1107 25 3.6625330740886817 1108 1 5.264500748929361 1108 2 10.771764945819392 1108 3 0.44034659071334303 1108 4 6.122860236943726 1108 5 7.603616885789176 1108 6 7.41174257888612 1108 7 7.633325403493032 1108 8 7.102041184748689 1108 9 5.761475595058599 1108 10 4.845589074177109 1108 11 7.06600532103354 1108 12 2.517861270063314 1108 13 3.4987721539866357 1108 14 5.0474150646007985 1108 15 5.152063908132774 1108 16 4.983894806576167 1108 17 0.8423440844188157 1108 18 4.018493736565244 1108 19 4.97666957232877 1108 20 5.181718285373467 1108 21 5.031817323874698 1108 22 2.04625265656108 1108 23 4.7937825963482545 1108 24 5.5118360227807415 1108 25 6.2094417063536085 1109 1 3.3182502301084185 1109 2 -14.194706135488236 1109 3 -6.027814575161177 1109 4 1.5674391017819325 1109 5 0.7429115547058487 1109 6 2.1333906269766736 1109 7 3.478041834211357 1109 8 3.614120220991328 1109 9 2.9949652934678923 1109 10 2.24867995686202 1109 11 0.7299656407230799 1109 12 1.2378527772501364 1109 13 2.7497517395344033 1109 14 3.3114457153994685 1109 15 3.211914649890062 1109 16 3.0051732845932726 1109 17 3.5791560799276496 1109 18 3.8246725530415944 1109 19 2.27898275808473 1109 20 1.2129824538850364 1109 21 0.5073632562079133 1109 22 2.5302058841618376 1109 23 2.7007516425507774 1109 24 3.8846867402096628 1109 25 4.279159445643412 1110 1 2.925110388419764 1110 2 8.926665896174033 1110 3 6.245712249816837 1110 4 7.602619400814476 1110 5 8.041944096482453 1110 6 4.085017715806927 1110 7 6.463969714598342 1110 8 7.179643527939437 1110 9 5.389864150108174 1110 10 4.869612234786384 1110 11 2.8865179714178915 1110 12 5.542162363881623 1110 13 10.03383265422218 1110 14 7.4636005087152135 1110 15 6.656065925175902 1110 16 6.218893601215967 1110 17 6.936236115107949 1110 18 6.771902104492781 1110 19 5.732808615604522 1110 20 5.843589314043346 1110 21 3.801998260605451 1110 22 5.8609006950677305 1110 23 6.355105204556054 1110 24 7.590051735812125 1110 25 7.852598319873845 1111 1 5.176496666898334 1111 2 9.686287015196697 1111 3 7.4713751936367885 1111 4 8.01376279065315 1111 5 8.338182244215613 1111 6 7.70063454572464 1111 7 7.478895739585994 1111 8 7.831353296496442 1111 9 6.309328222830189 1111 10 5.493351707804781 1111 11 7.234946002405469 1111 12 6.2984348786706015 1111 13 8.214022501332455 1111 14 6.1389492996936355 1111 15 5.8610483952666215 1111 16 5.765539876562045 1111 17 6.913984877171057 1111 18 6.566525060842707 1111 19 5.688681135212715 1111 20 5.1407551614427724 1111 21 7.989550217810784 1111 22 7.282162185893142 1111 23 7.192427947655538 1111 24 7.209614301323384 1111 25 7.690693644026563 1112 1 -2.428063621405876 1112 2 7.7041604730557935 1112 3 5.981762624815207 1112 4 -0.48633200697257806 1112 5 3.866902939059816 1112 6 3.0218066623839412 1112 7 5.602629780741598 1112 8 5.777105915000938 1112 9 4.542051915369392 1112 10 3.2344989531499913 1112 11 -0.08413086279379911 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6.3221151498616734 2204 5 1.7296806050632902 2204 6 4.23214578562629 2204 7 5.422125455075982 2204 8 5.375245990560369 2204 9 3.7919546717237615 2204 10 3.429811138784757 2204 11 5.030874465114753 2204 12 5.052026378494979 2204 13 2.9328742165882815 2204 14 4.441533891661788 2204 15 4.5578813188118765 2204 16 4.659283290682172 2204 17 -0.9221496504018276 2204 18 2.9563506082024986 2204 19 3.8331987561002023 2204 20 4.207139367039311 2204 21 5.677818570462701 2204 22 5.129003890611557 2204 23 3.829926484126677 2204 24 5.745481003114744 2204 25 5.59355630647827 2205 1 5.94126296734105 2205 2 10.339134812058294 2205 3 7.167136962637553 2205 4 7.53864081258023 2205 5 8.065230954319222 2205 6 7.445200581929818 2205 7 7.5939984213651925 2205 8 7.241235838276104 2205 9 6.067120622524229 2205 10 5.492169592513772 2205 11 7.627208362463879 2205 12 6.79244027931497 2205 13 9.881869834619433 2205 14 7.450427986850297 2205 15 6.383097163317061 2205 16 5.858398219746341 2205 17 6.71860265989363 2205 18 6.556940655493058 2205 19 4.9116924000760775 2205 20 5.412063658765629 2205 21 -1.3641572202440528 2205 22 3.471361476623965 2205 23 4.964097163982636 2205 24 7.072345509167064 2205 25 7.245215814482954 2206 1 3.7952873418175557 2206 2 8.452303831289171 2206 3 5.8863048800701385 2206 4 1.5552085422573931 2206 5 5.523361213860177 2206 6 5.961121848323284 2206 7 5.96844541418664 2206 8 5.922200586352172 2206 9 4.4813489983273875 2206 10 3.6153081998632146 2206 11 3.2824986806924557 2206 12 4.409429053339515 2206 13 7.64759154060793 2206 14 -0.3224863258467998 2206 15 1.9161159782093264 2206 16 3.0871647115868477 2206 17 4.211332758661978 2206 18 -1.9503137868339877 2206 19 0.47484483611837547 2206 20 2.180664937076507 2206 21 3.222051469346763 2206 22 -2.562118294696674 2206 23 -3.5038185821765317 2206 24 0.759741161560946 2206 25 2.553889384253207 2207 1 1.4416924870599104 2207 2 -14.194706135488236 2207 3 -6.477172985733308 2207 4 -4.466773739027214 2207 5 0.8818173572000418 2207 6 1.6449662984468034 2207 7 2.8007579337084607 2207 8 3.114562229876608 2207 9 0.3406726204417083 2207 10 0.028956437930055756 2207 11 -2.2853648511245623 2207 12 -3.0317343052657044 2207 13 -0.1286642449501798 2207 14 -5.910602860474963 2207 15 -1.871178510247951 2207 16 -0.8945628631639835 2207 17 0.35733752908535266 2207 18 0.3349830868349777 2207 19 -0.29073286783142294 2207 20 -0.39286063296069074 2207 21 -4.298249805605675 2207 22 -4.5282610666534 2207 23 -1.808535054334861 2207 24 -0.002382244748506679 2207 25 0.5046674175893813 2208 1 4.773515345849059 2208 2 6.351040252954782 2208 3 6.123659764537646 2208 4 7.991385704525488 2208 5 3.682540354552252 2208 6 5.957342311946412 2208 7 6.377428578918433 2208 8 6.37535299965614 2208 9 4.876137904221601 2208 10 4.302054625634987 2208 11 6.37235832457614 2208 12 5.768242372239062 2208 13 8.712580901912943 2208 14 3.34435454167345 2208 15 4.668019744680638 2208 16 4.95294917535929 2208 17 5.719909039971469 2208 18 0.6891091106239244 2208 19 3.1223400767021943 2208 20 4.266045130439455 2208 21 7.025412923675031 2208 22 6.1829543097305155 2208 23 2.235813356305684 2208 24 4.067236775432313 2208 25 5.111739621290798 2209 1 2.883359153726577 2209 2 4.653195962413861 2209 3 4.9072287236684 2209 4 1.2810118087583326 2209 5 5.083254808637495 2209 6 5.670549049029553 2209 7 6.253419161369688 2209 8 4.851236666551046 2209 9 4.549646979416067 2209 10 3.5222936562672764 2209 11 -0.473826389586101 2209 12 3.295441296909896 2209 13 2.271122079836143 2209 14 3.646564971692868 2209 15 4.350091886022444 2209 16 3.5800874864863665 2209 17 1.014796736544561 2209 18 3.650309427161318 2209 19 4.240230613132059 2209 20 4.350515435415378 2209 21 6.574963092607224 2209 22 5.301692313299981 2209 23 0.9455053497055417 2209 24 -2.9482252905915516 2209 25 0.5672742551962173 2210 1 -2.198493993126572 2210 2 -14.194706135488236 2210 3 -7.813855192415515 2210 4 -2.158288890542366 2210 5 -0.4034584780757946 2210 6 -1.1180173845368806 2210 7 -1.0017206987701712 2210 8 -21.491420676106298 2210 9 -13.259653719884632 2210 10 -6.964244805271186 2210 11 -10.356903312663025 2210 12 -4.6596286331600325 2210 13 -5.357724074010012 2210 14 -3.8554280353001396 2210 15 -5.5116913307607724 2210 16 -4.9336537722548925 2210 17 -4.603804662056838 2210 18 -4.6537115518596615 2210 19 -10.314874281972838 2210 20 -6.9199391100391665 2210 21 -5.0825543899102605 2210 22 -9.927258735651067 2210 23 -7.556382762182569 2210 24 -10.632001514367776 2210 25 -8.131921548999586 2211 1 -0.17951198134952762 2211 2 4.888701140465624 2211 3 0.39100969425061294 2211 4 1.2619159977370558 2211 5 0.8456461440101961 2211 6 -0.27922090151679413 2211 7 -21.23172069877017 2211 8 -13.974118102903104 2211 9 -9.79608027469628 2211 10 -7.763506723477203 2211 11 -10.805288405830726 2211 12 -5.3949032963850065 2211 13 -7.499516443131572 2211 14 -5.396979217037657 2211 15 -5.5617889350198695 2211 16 -12.174065968754046 2211 17 -8.229150310197518 2211 18 -6.290587408921851 2211 19 -9.095928569300415 2211 20 -7.1735528875659895 2211 21 -5.083756293596636 2211 22 -6.622006658535636 2211 23 -8.219641615130865 2211 24 -22.80836515073141 2211 25 -22.63464882172686 2212 1 -1.8577836640291459 2212 2 -14.194706135488236 2212 3 -8.331639486974003 2212 4 -20.100107072360547 2212 5 -20.635276659893975 2212 6 -21.365290111809607 2212 7 -10.524731451458344 2212 8 -10.239628561410957 2212 9 -6.108679461168435 2212 10 -6.273759305108268 2212 11 -6.567154208720371 2212 12 -3.7672043907357917 2212 13 -7.1277957585978235 2212 14 -4.836519596069117 2212 15 -4.748859789542134 2212 16 -4.890082576425634 2212 17 -5.108226297768798 2212 18 -4.126569160201139 2212 19 -4.824066201164758 2212 20 -4.902620765301467 2212 21 -2.561635523830102 2212 22 -3.948953098635755 2212 23 -4.300139686262074 2212 24 -3.0995837887242423 2212 25 -3.6471936245942445 2213 1 -19.183948538581117 2213 2 -14.194706135488236 2213 3 -8.599241177634996 2213 4 -5.610391107620096 2213 5 -20.635276659893975 2213 6 -10.7502068600956 2213 7 -21.23172069877017 2213 8 -10.737258090406003 2213 9 -8.12011494328415 2213 10 -7.059369905440363 2213 11 -6.7913695222614585 2213 12 -5.135062699583326 2213 13 -19.75772407401001 2213 14 -12.577272038505551 2213 15 -6.787479773464003 2213 16 -6.1874130100787745 2213 17 -4.672314144738699 2213 18 -4.740084092149928 2213 19 -5.688197227391768 2213 20 -5.539665759491577 2213 21 -3.618811179154317 2213 22 -4.429544373617411 2213 23 -4.2098116643284165 2213 24 -3.466543407342577 2213 25 -4.217410819767995 2214 1 -19.183948538581117 2214 2 -14.194706135488236 2214 3 -12.09506165883603 2214 4 -20.100107072360547 2214 5 -20.635276659893975 2214 6 -21.365290111809607 2214 7 -16.56761532006545 2214 8 -14.870125396194114 2214 9 -13.366273817679263 2214 10 -13.220691267700087 2214 11 -14.248988932860609 2214 12 -11.878528942510398 2214 13 -19.75772407401001 2214 14 -16.787044640429365 2214 15 -24.491691330760773 2214 16 -18.272169888410964 2214 17 -24.22471375296593 2214 18 -17.854245430714066 2214 19 -14.65983885636661 2214 20 -13.18145492682392 2214 21 -10.704418475330876 2214 22 -11.823124417718725 2214 23 -11.747986394554589 2214 24 -10.575653844474552 2214 25 -10.288874946864071 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 109342.0 3 3.0 4 497.0 5 1937.0 6 2618.0 7 2534.0 8 2273.0 9 2253.0 10 2056.0 11 1956.0 12 1798.0 13 1923.0 14 3097.0 15 6601.0 16 9803.0 17 10588.0 18 10645.0 19 12475.0 20 14797.0 21 17104.0 22 18236.0 23 19540.0 24 20547.0 25 20944.0 26 17793.0 27 13595.0 28 14537.0 29 17041.0 30 16581.0 31 19081.0 32 25184.0 33 31984.0 34 38885.0 35 45739.0 36 46019.0 37 48007.0 38 37148.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.85769497777455 18.286516562878845 11.755827487712972 26.099960971633628 2 14.573671907401122 19.945896014779628 40.79855219997738 24.681879877841872 3 19.444161596619402 23.424095664791388 28.584565842730953 28.54717689585826 4 11.387129419239042 16.646386235460145 38.338520610530665 33.62796373477015 5 12.438621792087295 38.28644901221483 36.0535506402794 13.221378555418484 6 31.795911263626632 37.38526908695201 18.30789812791497 12.510921521506388 7 28.09428726588784 30.96986012874513 22.496125725184772 18.439726880182256 8 24.696715989474765 37.50827877882521 20.25847689996957 17.536528331730462 9 25.91856731945868 15.511975881163858 19.90074912286247 38.66870767651499 10 13.609813974909946 29.982095961983656 35.28574783672249 21.122342226383903 11 33.6580358705858 22.594649649092098 24.429237551124338 19.318076929197765 12 22.891278831197337 24.807314877549917 32.064935601975996 20.23647068927675 13 28.079180326404458 21.379581409059647 25.539407040831826 25.001831223704073 14 19.895796578791806 22.293300095771833 28.040832099908197 29.77007122552816 15 22.227141497093587 31.30134600648622 23.6017864191893 22.869726077230894 16 21.565672367699584 28.749841236404418 27.680161830443577 22.00432456545242 17 21.041660541102665 28.60527484567448 28.83152094014382 21.521543673079037 18 20.948810210490656 28.014148683250777 30.28004382551585 20.756997280742713 19 22.377078433705172 27.58433461143089 27.58585978751247 22.452727167351473 20 22.608651074146895 27.93596130417476 28.551439634271375 20.90394798740697 21 21.784278625714464 26.780981094152377 27.152974059418376 24.28176622071478 22 21.811738107604413 28.411139286914544 28.295957960343404 21.48116464513764 23 21.546725989789035 27.96244340622291 28.383434881032656 22.1073957229554 24 21.7716251080491 27.541571013256778 28.842126836008326 21.8446770426858 25 22.078442056526328 27.673576042013178 28.389449743588973 21.858532157871522 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 542.0 1 542.0 2 1319.5 3 2097.0 4 2097.0 5 2097.0 6 3012.0 7 3927.0 8 3927.0 9 3927.0 10 4550.5 11 5174.0 12 5174.0 13 5174.0 14 6289.0 15 7404.0 16 7404.0 17 7404.0 18 10629.5 19 13855.0 20 13855.0 21 13855.0 22 19535.5 23 25216.0 24 25216.0 25 25216.0 26 33036.0 27 40856.0 28 40856.0 29 40856.0 30 49880.0 31 58904.0 32 58904.0 33 58904.0 34 66031.5 35 73159.0 36 73159.0 37 73159.0 38 77278.5 39 81398.0 40 81398.0 41 81398.0 42 83266.0 43 85134.0 44 85134.0 45 85134.0 46 83298.0 47 81462.0 48 81462.0 49 81462.0 50 75130.0 51 68798.0 52 68798.0 53 68798.0 54 59402.5 55 50007.0 56 50007.0 57 50007.0 58 41280.0 59 32553.0 60 32553.0 61 32553.0 62 25681.0 63 18809.0 64 18809.0 65 18809.0 66 14200.5 67 9592.0 68 9592.0 69 9592.0 70 6824.0 71 4056.0 72 4056.0 73 4056.0 74 2780.0 75 1504.0 76 1504.0 77 1504.0 78 1010.0 79 516.0 80 516.0 81 516.0 82 334.0 83 152.0 84 152.0 85 152.0 86 93.5 87 35.0 88 35.0 89 35.0 90 22.5 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.943482856030345 2 36.200709301958476 3 5.507388436784478 4 13.391043672133513 5 13.475083476030617 6 14.482208066919137 7 9.197322152080474 8 7.612893720467677 9 0.11786620081453963 10 1.4196562937394104 11 5.531142084397612 12 5.474614416660026 13 15.849696539634763 14 1.575558398643336 15 2.8829110546168524 16 2.9386870246451613 17 2.850738392659822 18 1.4785893941466803 19 1.4280753080833062 20 0.2912076925736777 21 4.257766164883389 22 1.5847291106965082 23 0.11681382402155267 24 9.037210540004601 25 0.11997095440051356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 665161.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.55737637786551 #Duplication Level Percentage of deduplicated Percentage of total 1 97.30186398874594 92.00608975451726 2 2.1002510633895377 3.971884605778736 3 0.2694825544437634 0.7644468998342271 4 0.12106077718822823 0.4578875789273684 5 0.05582987099456575 0.2639563062380415 6 0.03636465939329625 0.20631280710628774 7 0.022745521935757303 0.15055298150132793 8 0.01781607512799596 0.13477130571633938 9 0.010101894893326023 0.08596878098020796 >10 0.05494552793016241 0.9044569931488692 >50 0.006466572344356758 0.42679662869403073 >100 0.0029098360547511162 0.5490700523445269 >500 1.616575582534251E-4 0.07780530521268779 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.5033954185528014E-4 0.0 8 0.0 0.0 0.0 1.5033954185528014E-4 0.0 9 0.0 0.0 0.0 1.5033954185528014E-4 0.0 10 0.0 0.0 0.0 1.5033954185528014E-4 0.0 11 0.0 0.0 0.0 3.006790837105603E-4 0.0 12 0.0 0.0 0.0 3.006790837105603E-4 0.0 13 0.0 0.0 0.0 3.006790837105603E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGTA 55 7.016621E-5 15.586463 4 GTCTAGG 60 4.5638668E-4 15.558223 1 CTACACT 90 7.406925E-9 15.478224 4 CCAACGA 45 8.951241E-5 15.149238 19 TCTGGAC 65 1.4429894E-5 15.141975 3 TCCTATT 55 0.0038800356 14.92183 2 TCCTATA 65 6.910869E-4 14.730525 2 CTAGGAT 60 1.2386456E-4 14.5811615 3 GGACGTG 260 0.0 14.425907 6 GTCCTAT 65 8.376219E-4 14.361437 1 GTGTAGC 105 9.3315975E-7 13.970649 1 AGGACGT 270 0.0 13.897341 5 TGTAGGA 325 0.0 13.888781 2 TTTAGGA 180 7.2759576E-12 13.678345 2 GTCCTAC 380 0.0 13.33562 1 GTATTAA 60 0.008174805 13.335619 1 CTATATG 60 0.008174805 13.335619 1 GACGTGG 115 1.875378E-9 13.283068 7 GTAGGAC 380 0.0 13.238161 3 TAGGACG 310 0.0 13.1353655 4 >>END_MODULE