FastQCFastQC Report
Thu 26 May 2016
SRR1926442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926442_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665161
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA10480.15755583986433358No Hit
GTCCTACAGTGGACATTTCTAAATT9140.13741034125572604No Hit
GTATCAACGCAGAGTACTTTTTTTT8540.12838996874440925No Hit
GTCCTACAGTGTGCATTTCTCATTT8070.12132401027721108No Hit
CCCATGTACTCTGCGTTGATACCAC7920.11906891714938186No Hit
GTACATGGGAAGCAGTGGTATCAAC7270.10929684692878867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2850.013.3832954
CCAAATC500.001454664513.3518583
TGTAGGA6250.013.2775712
CTGTAGG6600.012.8547191
GTAGGAC5750.012.7713423
GATTAAG603.9696935E-412.7102841
GGACGTG4450.012.4284196
GGCTTAG550.00298732512.1325441
TGTGCGC550.003107869312.068645510
GGCGAGG2050.012.01574619
GTAGGAT802.748833E-511.9213023
TCTAAAC657.755335E-411.7378983
TAGGACG5100.011.5922964
GTGTAAG1001.8459341E-611.4392561
TGGCGAG5500.011.36959918
TCTAGGA1104.6989226E-711.2727562
AGGACGT4950.011.1730245
AGGGCAC855.412657E-511.1558910
TAGAGGT600.00570843811.1248654
GGACCTG4300.011.0879056
Produced by FastQC (version 0.11.5)