FastQCFastQC Report
Thu 26 May 2016
SRR1926441_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926441_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678731
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAC250.0073713818.34881610
GTCCTAA1950.016.3448621
GGGTATA500.002503880515.9362391
TGTAGGA3800.015.0513692
GTCCTAC3900.014.6422721
TAGGCCT450.005305414614.2365194
GCAGGAC550.00109137613.8687753
GGCGAGG1700.013.58723319
AGACTAG704.6903704E-513.3627615
TCCTACA4400.013.3084082
GCCTTAG600.00840352613.28021
CTGTAGG3600.013.28021
GTCTAAA700.00151294113.28021
TAGATGT652.9924623E-413.1414024
TCTATAC600.002102283312.7130443
TGGACTG758.952996E-512.4719095
CTATACT600.002449495712.4569544
GTGTAGG750.002541551812.3948531
GTAGGAC4250.012.3070823
TATAGGA1001.05260646E-412.2561152
Produced by FastQC (version 0.11.5)