FastQCFastQC Report
Thu 26 May 2016
SRR1926441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926441_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678731
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA12650.1863772245558255No Hit
GTCCTACAGTGGACATTTCTAAATT12580.1853458881353585No Hit
GTCCTACAGTGTGCATTTCTCATTT9330.13746241147081834No Hit
GTATCAACGCAGAGTACTTTTTTTT8990.1324530631428357No Hit
CTGTAGGACCTGGAATATGGCGAGA8750.12891705255837732No Hit
GTCCTAAAGTGTGTATTTCTCATTT8440.12434970555345196No Hit
CTTTAGGACGTGAAATATGGCGAGG8070.118898355902412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACCT551.0875292E-515.6031955
TAGGACT1101.0913936E-1114.7363514
GGCGAGG2250.014.3197119
TAAGTAG551.896957E-413.8695075
TCTAAAC500.001455112113.3513783
TGGCGAG6650.012.82503918
TAGCACC603.9608523E-412.7137154
GTGCTAT603.963331E-412.7127711
TAGGACC3650.012.5395534
TGTAGGA7950.012.1185032
GTAGGAC8050.011.8468323
CTGTAGG8150.011.8158581
CACTGTT1701.8189894E-1211.7353857
TATGGCG7600.011.7206616
GACGTGG3000.011.7167587
GATATAC1800.011.6533741
ATGGCGA7650.011.52018217
AGGACGT5800.011.5081035
GTCCTAC6400.011.471291
CTATTCT752.000413E-411.4423424
Produced by FastQC (version 0.11.5)