##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926441_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 678731 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.223194461428754 31.0 28.0 31.0 19.0 34.0 2 28.71491503997902 31.0 28.0 33.0 22.0 34.0 3 29.079230210495762 31.0 28.0 33.0 24.0 34.0 4 32.83597920236441 35.0 33.0 37.0 27.0 37.0 5 32.509197310863954 35.0 33.0 37.0 25.0 37.0 6 32.576079477731234 35.0 33.0 37.0 25.0 37.0 7 32.41632988621413 35.0 33.0 37.0 23.0 37.0 8 32.35721810260619 35.0 33.0 37.0 23.0 37.0 9 33.59044157405511 37.0 33.0 39.0 22.0 39.0 10 33.49788355033143 37.0 33.0 39.0 20.0 39.0 11 33.715687068956626 37.0 34.0 39.0 24.0 39.0 12 33.51614999167564 37.0 33.0 39.0 22.0 39.0 13 33.652810907413986 37.0 33.0 39.0 23.0 39.0 14 34.304419571229246 38.0 33.0 40.0 18.0 41.0 15 34.422650505133845 38.0 33.0 40.0 22.0 41.0 16 34.39808849161155 38.0 34.0 40.0 22.0 41.0 17 34.31013317499864 38.0 33.0 40.0 19.0 41.0 18 34.27894703498146 38.0 33.0 40.0 19.0 41.0 19 34.312268041389004 39.0 34.0 40.0 18.0 41.0 20 34.29428448089155 39.0 34.0 40.0 18.0 41.0 21 34.21528263774603 39.0 34.0 40.0 18.0 41.0 22 34.15095965853924 39.0 34.0 40.0 18.0 41.0 23 33.7934925618544 39.0 33.0 40.0 10.0 41.0 24 33.85742068654592 39.0 34.0 40.0 10.0 41.0 25 33.64365558667572 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1165776958719853 1101 2 1.0280573129343686 1101 3 0.9156953434403974 1101 4 0.8264617382569881 1101 5 1.1135865214540033 1101 6 0.6573367176952161 1101 7 0.7800594720720966 1101 8 1.0035005551894542 1101 9 1.574268635895919 1101 10 1.4337167778884279 1101 11 1.0799529208725076 1101 12 1.2410498559998189 1101 13 1.0442356347727255 1101 14 1.428155890674411 1101 15 1.298380763572851 1101 16 1.2366220179547014 1101 17 1.0747982642187424 1101 18 1.5748341934021397 1101 19 1.1384451233157975 1101 20 1.2746584494308308 1101 21 1.4425155557783995 1101 22 1.4589582043009912 1101 23 1.3751449045764943 1101 24 1.2607760953728118 1101 25 1.1023971055411579 1102 1 0.9650488194429556 1102 2 0.9157847425482935 1102 3 0.7139604332147833 1102 4 0.593706943128538 1102 5 0.9261359167360013 1102 6 0.5328930089459973 1102 7 0.8628364554555574 1102 8 0.9475924920480239 1102 9 1.204646117986826 1102 10 1.1794569851196854 1102 11 0.9978289061366894 1102 12 0.9075371382555559 1102 13 0.9162058813793266 1102 14 1.274996353598894 1102 15 1.3053344922562786 1102 16 0.9596337037690077 1102 17 1.0675007936798124 1102 18 1.0500312462383619 1102 19 1.0898676369985552 1102 20 1.316296294845607 1102 21 1.3675326372128183 1102 22 1.1778992258059944 1102 23 1.5655631003543888 1102 24 1.4495791270632736 1102 25 1.5587313240567298 1103 1 0.7600147198043636 1103 2 0.6792441034400447 1103 3 0.6824209810638706 1103 4 0.5516816507834008 1103 5 0.8764937896244263 1103 6 0.43922771971005403 1103 7 0.8656263303943703 1103 8 0.8317132437736632 1103 9 1.1140298035208644 1103 10 1.2804660038946167 1103 11 1.005929627344571 1103 12 1.1663729677006387 1103 13 0.8929751305710454 1103 14 1.3588060454731732 1103 15 1.267489810700475 1103 16 1.4334670717656977 1103 17 1.2605567781506295 1103 18 1.3878028896525691 1103 19 1.2644070870486601 1103 20 1.4149306986814096 1103 21 1.5499557248284788 1103 22 1.6594250577090222 1103 23 1.7347055709483072 1103 24 1.5386079298609943 1103 25 1.6473280603792375 1104 1 0.6411671167025581 1104 2 0.8863926802763267 1104 3 0.6730822816181607 1104 4 0.5995884163140985 1104 5 0.6371194200906345 1104 6 0.11683321338892938 1104 7 0.4479139997752881 1104 8 0.6271589812154659 1104 9 0.7495397401882613 1104 10 1.0829618701161863 1104 11 0.6485257441927104 1104 12 0.7452794316475035 1104 13 0.8005032870618578 1104 14 1.0060839913752702 1104 15 0.8050023333625944 1104 16 0.8754309025043696 1104 17 0.9523672171111031 1104 18 1.069066907234557 1104 19 1.1718354088906082 1104 20 1.1645918501770183 1104 21 1.1858573738334286 1104 22 1.001267908265568 1104 23 1.0463851849210002 1104 24 0.9621952522471773 1104 25 1.2642507266576573 1105 1 0.9228682922440896 1105 2 0.8392622623598811 1105 3 0.8888845523459992 1105 4 0.7142158382275241 1105 5 0.7802452383631362 1105 6 0.35163533864609775 1105 7 0.4940337429156543 1105 8 0.4468543240759786 1105 9 0.9908859673026029 1105 10 1.0345360705470554 1105 11 0.6815690598155655 1105 12 0.6108202015336914 1105 13 0.7381933514153047 1105 14 0.9435274483124871 1105 15 0.8972584448623948 1105 16 0.9003458714359951 1105 17 0.9012737625983931 1105 18 0.6812005462872008 1105 19 0.7156227723386763 1105 20 1.0020907245111417 1105 21 0.7481257464322937 1105 22 0.7023007500004113 1105 23 1.0967228846833947 1105 24 0.8147371789715265 1105 25 1.1055283747433293 1106 1 0.827395816223575 1106 2 0.9097341215888619 1106 3 0.731033892653187 1106 4 0.638738373048831 1106 5 0.7070183769548208 1106 6 -0.18035284088684023 1106 7 -0.01575816532769636 1106 8 0.1434696462741698 1106 9 0.17802600091089005 1106 10 0.35262917043164776 1106 11 0.1428235863800893 1106 12 0.2537321703048505 1106 13 -0.05119564118593445 1106 14 0.4806624105237276 1106 15 0.293531927486697 1106 16 0.34786513589153145 1106 17 0.28695899057824903 1106 18 0.13763724565752256 1106 19 0.0859868986466168 1106 20 0.18650042760140906 1106 21 0.14072863411318792 1106 22 0.24617929963567775 1106 23 0.4248792810857225 1106 24 0.3053235695348917 1106 25 0.20513061273714328 1107 1 0.8063069208953273 1107 2 0.8646586201239082 1107 3 0.7578055208446948 1107 4 0.5609388624399898 1107 5 0.5838012740216953 1107 6 0.35077742731239425 1107 7 0.5530986877583643 1107 8 0.6114137204555661 1107 9 0.6841192428053517 1107 10 0.4448045463291379 1107 11 0.3999109040657842 1107 12 0.6631787075404318 1107 13 0.43172396343751984 1107 14 0.9302100456697389 1107 15 0.8111958896232707 1107 16 0.7632560048828054 1107 17 0.7945695060488092 1107 18 0.8610386751948553 1107 19 0.766876495864814 1107 20 0.6259422963029095 1107 21 0.8628703643685043 1107 22 0.7205828541609378 1107 23 0.7549768373496022 1107 24 0.97216731852874 1107 25 1.0320889822918602 1108 1 1.0084716524718225 1108 2 1.0484841568906234 1108 3 0.8750832263576918 1108 4 0.5665735077805181 1108 5 -0.4669446590771784 1108 6 0.33975504129001166 1108 7 0.8262896594787534 1108 8 0.9132335884519023 1108 9 1.1798516061847693 1108 10 1.3181585152713424 1108 11 0.9420352476607192 1108 12 1.1718986574368557 1108 13 0.7887280263239376 1108 14 1.2334250253553094 1108 15 1.152917959286519 1108 16 1.0507473732565273 1108 17 1.1548194618870014 1108 18 1.1992438589882752 1108 19 1.2409454846396883 1108 20 1.359259169325668 1108 21 1.2917939403509067 1108 22 1.2202456185295887 1108 23 1.566582560720036 1108 24 1.5019725248045361 1108 25 1.5598386571003573 1109 1 0.5003855473707937 1109 2 -0.4030225817100579 1109 3 0.5736283673962497 1109 4 0.644284389705625 1109 5 -0.7848456872503746 1109 6 0.11461754047208927 1109 7 0.6397609646094082 1109 8 0.7803850403267987 1109 9 1.094298737488792 1109 10 -0.9985546381406749 1109 11 0.32714971003593263 1109 12 0.8775480373619118 1109 13 0.7734467221927659 1109 14 0.9744496320515594 1109 15 0.9023943092896474 1109 16 1.026437645698337 1109 17 1.0581169648333173 1109 18 0.8983886898275699 1109 19 1.0850649652824345 1109 20 1.022281677826662 1109 21 0.9251569705532674 1109 22 1.0166027546584502 1109 23 1.0258284615813906 1109 24 0.9349514371513123 1109 25 0.9549745544311605 1110 1 0.7145194812818012 1110 2 -0.45020355663085 1110 3 0.2585159499438703 1110 4 0.6273995046678991 1110 5 1.0131846215636102 1110 6 0.6305799280228257 1110 7 0.810238500205088 1110 8 1.1117334135501906 1110 9 1.3319595383438525 1110 10 1.0860733727002625 1110 11 1.0656184914485678 1110 12 1.070254581368225 1110 13 0.9852903175153855 1110 14 1.2727789572923598 1110 15 0.9383271769208008 1110 16 0.7005606368805317 1110 17 0.8036985199732172 1110 18 0.9949900818444704 1110 19 0.9537903918579929 1110 20 0.8488394261693486 1110 21 0.8178291594807519 1110 22 0.9528931695914551 1110 23 1.1816605543146537 1110 24 0.9706043745105504 1110 25 1.1518673284129193 1111 1 0.09474113516074212 1111 2 0.25397376647230274 1111 3 0.3011416958944224 1111 4 0.006768643627829363 1111 5 0.3601581936011229 1111 6 -0.2108522970411144 1111 7 0.027629804552908865 1111 8 0.2651860989721868 1111 9 0.4964949177129938 1111 10 0.42196425079063005 1111 11 -0.1389850891140938 1111 12 0.278268221606929 1111 13 0.08503456303968449 1111 14 0.33160248670412784 1111 15 0.17958889900094022 1111 16 0.11658791735793983 1111 17 -0.0036331083302769684 1111 18 0.3658340716142945 1111 19 0.18823199458604023 1111 20 0.033835163594751805 1111 21 0.31058278266915806 1111 22 0.20353255578071838 1111 23 0.3794440155252232 1111 24 0.40964955780125933 1111 25 0.5158911988306514 1112 1 0.2181162788542821 1112 2 -0.12113643800751106 1112 3 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15 -0.34272017118307474 1113 16 -0.4479781142330097 1113 17 -0.38427862496389764 1113 18 -0.25289060438214506 1113 19 -3.482572722024589 1113 20 -1.1336504921233228 1113 21 -0.8129576447377573 1113 22 -0.7030236670992522 1113 23 -0.058252948946936556 1113 24 -0.7634205495773045 1113 25 -0.012964994354980774 1114 1 -0.2031702032876801 1114 2 -1.4317466086342598 1114 3 -1.1232402307892926 1114 4 -0.5205970852724278 1114 5 -0.37927062140314405 1114 6 -0.755268324321591 1114 7 -0.620920920084771 1114 8 -0.7880960834660051 1114 9 -0.9681683388939959 1114 10 -1.1994764994225022 1114 11 -0.8525400763263704 1114 12 -0.7387820767732904 1114 13 -1.9355962980003838 1114 14 -1.2175023726309107 1114 15 -1.0255688162590815 1114 16 -1.1565152369472642 1114 17 -4.900691931859686 1114 18 -2.1678828934325978 1114 19 -1.7380254649195024 1114 20 -1.595789729840881 1114 21 -1.1766721192916236 1114 22 -1.2088289105449448 1114 23 -4.152866299905298 1114 24 -2.2987477141509984 1114 25 -1.572812978492454 1115 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0.12508853076714388 2203 4 -0.2530871797558163 2203 5 0.02496081210290413 2203 6 0.13654311528015484 2203 7 0.2883801060251585 2203 8 0.41580516615301377 2203 9 0.7129299035117711 2203 10 0.7842124165584039 2203 11 0.7622115237955427 2203 12 0.6556098768993692 2203 13 0.3599132011360595 2203 14 0.860227994127186 2203 15 0.8857539511898693 2203 16 0.8104003375727018 2203 17 0.9258599324093382 2203 18 0.9464166087516972 2203 19 1.11613485205703 2203 20 1.051121780877395 2203 21 1.075949032796153 2203 22 1.1013610721838987 2203 23 1.1369741958785085 2203 24 1.2032791069401299 2203 25 1.04971572210696 2204 1 0.18367701084549992 2204 2 0.1953549250485196 2204 3 0.04970676979311861 2204 4 -0.22246080105576738 2204 5 -0.03735642998400124 2204 6 -0.17796294672501034 2204 7 0.06734349459199507 2204 8 0.019077280929259643 2204 9 0.2403542780735819 2204 10 0.1291781307464035 2204 11 -0.11928439781734568 2204 12 0.04815249903722929 2204 13 -0.10985372374295821 2204 14 0.2906902113333629 2204 15 0.2798184871771454 2204 16 0.16020967083763793 2204 17 0.09527972412944052 2204 18 0.1925519735835195 2204 19 0.1813591205978753 2204 20 0.09501788033190195 2204 21 0.28272411444939394 2204 22 0.07318429077518829 2204 23 0.37534181502384456 2204 24 0.40745496494044176 2204 25 0.36090862896240594 2205 1 0.9395429899209198 2205 2 0.6369265351940001 2205 3 0.6643729992942369 2205 4 0.5505490837430145 2205 5 0.5388405805592456 2205 6 0.4569485002659732 2205 7 0.321582246349422 2205 8 0.665249113975193 2205 9 0.9883681432376932 2205 10 0.7888670145979901 2205 11 0.6917980186826895 2205 12 0.7670617979802046 2205 13 0.46276837902332346 2205 14 1.1657580124716134 2205 15 1.2609758424874968 2205 16 0.8932715297626928 2205 17 1.0071247516411859 2205 18 1.0147450064599823 2205 19 1.115156200224071 2205 20 1.2095835887424826 2205 21 1.090920428766026 2205 22 0.9590915037368788 2205 23 1.3603108761163796 2205 24 1.008660138605805 2205 25 1.0128418721640813 2206 1 0.4899607111637927 2206 2 0.6825344635359194 2206 3 0.8026589243456783 2206 4 0.7801685241241216 2206 5 0.887625094037439 2206 6 0.72910088011038 2206 7 0.8189676464978888 2206 8 0.8014469285649994 2206 9 1.3346824861571207 2206 10 1.217440615038484 2206 11 0.9070141900042543 2206 12 0.9480313713221946 2206 13 0.7610892315064888 2206 14 0.9973263559502499 2206 15 0.9566676191396581 2206 16 1.1253280905211582 2206 17 1.182268900007415 2206 18 0.8887048346254787 2206 19 0.9986477007089221 2206 20 0.9579447954971485 2206 21 1.2025890597151445 2206 22 1.0657332020931563 2206 23 1.3368637554151306 2206 24 1.234738582550385 2206 25 1.3683944017919174 2207 1 -0.49291078625805085 2207 2 0.20768685392895847 2207 3 0.5375775869806141 2207 4 0.7989644903749209 2207 5 0.42996452912574057 2207 6 0.521455937392858 2207 7 0.651327360838664 2207 8 0.5900927836186654 2207 9 0.9384056332497863 2207 10 0.579107236757082 2207 11 0.41057835249348784 2207 12 0.4815309467274034 2207 13 0.195892717459337 2207 14 -4.870746680931937 2207 15 -1.972697041023352 2207 16 -0.7331190436448765 2207 17 -0.6137098485463 2207 18 0.01651937846069984 2207 19 -0.07179754169162322 2207 20 0.03280437663816116 2207 21 0.3490167079072748 2207 22 0.3467176042367228 2207 23 0.5949950252594292 2207 24 0.5334744017091779 2207 25 0.29791166612535847 2208 1 0.9613015089641017 2208 2 1.088004858831706 2208 3 0.9939322432958839 2208 4 0.9929566801828003 2208 5 0.9337867034376259 2208 6 0.9279182624150124 2208 7 0.9477065308956227 2208 8 0.9952497389393926 2208 9 1.3146248420726607 2208 10 1.3865013949199536 2208 11 0.9363009377862781 2208 12 0.9923438130730915 2208 13 0.8640852306620346 2208 14 1.1878491947476277 2208 15 1.0989441099305068 2208 16 1.2829314971603978 2208 17 1.006701598800987 2208 18 1.1823356653284876 2208 19 0.9577407396514985 2208 20 1.0778645726444438 2208 21 1.1606670839055795 2208 22 1.1471158877565415 2208 23 1.450555705839676 2208 24 1.3724993196943842 2208 25 1.3300626303498078 2209 1 -0.5201544262827902 2209 2 -0.16570245318287746 2209 3 0.3119833187847796 2209 4 0.5152817392650988 2209 5 0.28709613095333 2209 6 0.44185854699248495 2209 7 0.7251272678279292 2209 8 0.7004618075567279 2209 9 1.0127354364979055 2209 10 0.6795421003053121 2209 11 0.7373082023167044 2209 12 0.55074864554134 2209 13 0.6769144132249778 2209 14 0.9107450188706778 2209 15 0.5786425855252872 2209 16 0.6480137211655475 2209 17 0.5498726127242151 2209 18 0.7582298138611208 2209 19 0.7051168305591062 2209 20 0.5138755758700952 2209 21 0.6645824802345643 2209 22 0.6183347569786193 2209 23 0.9241637923372537 2209 24 0.7587577044688913 2209 25 0.8789310610256678 2210 1 -0.3020727493807449 2210 2 -0.3319075685406112 2210 3 0.5218285429888745 2210 4 0.7733449691612861 2210 5 1.0659005814063391 2210 6 0.9530723253711244 2210 7 0.8592517459965876 2210 8 1.1385939955506785 2210 9 1.5218667796310257 2210 10 1.2086446594888258 2210 11 0.9777203860675385 2210 12 0.8433074754655152 2210 13 0.8779243341330627 2210 14 1.0016664889435702 2210 15 1.0235532206153692 2210 16 1.0475087607115015 2210 17 1.1195743836217318 2210 18 1.1898724475097495 2210 19 1.1368647896898096 2210 20 1.0872509491172693 2210 21 1.0577294025111286 2210 22 1.240483351673376 2210 23 1.398084826814049 2210 24 1.244872420470017 2210 25 1.2991360996462618 2211 1 -1.388393449212554 2211 2 -0.44258154501845226 2211 3 0.15228540115023037 2211 4 0.27021996443902196 2211 5 0.4528292064798194 2211 6 0.11715245121754236 2211 7 0.20373062728116764 2211 8 0.34215601737432877 2211 9 0.5309153909834947 2211 10 0.49117349551523404 2211 11 0.21112635410121783 2211 12 0.5995291513324119 2211 13 0.317243633152799 2211 14 0.6751843406086024 2211 15 0.4127575986442338 2211 16 0.4143379235940614 2211 17 0.47977953383502125 2211 18 0.560639599902089 2211 19 0.4688187185370154 2211 20 0.5813507649112353 2211 21 0.6357827421710311 2211 22 0.5348314451636043 2211 23 0.8385042242079663 2211 24 0.7829285708208218 2211 25 0.8002203312169698 2212 1 0.9465163498415663 2212 2 0.20160239704260974 2212 3 0.4316408335369708 2212 4 0.32536467420933946 2212 5 0.5991485615453485 2212 6 0.5843722565999414 2212 7 0.5218628364018372 2212 8 0.6677079077574817 2212 9 0.922527513159082 2212 10 0.3899760133765824 2212 11 0.3828933510410266 2212 12 0.7278358314912552 2212 13 0.5700875385893127 2212 14 0.40922071447248953 2212 15 0.5189458635027009 2212 16 0.23206659009971986 2212 17 0.6885272371578282 2212 18 0.6064371009950165 2212 19 0.5792350094301 2212 20 0.7091958167756331 2212 21 0.7809098122376454 2212 22 0.9827637478327915 2212 23 0.9376641218548798 2212 24 0.7988195898576365 2212 25 0.5286758941436318 2213 1 0.18962217057886477 2213 2 0.20510916134171353 2213 3 -0.2686870261082106 2213 4 -1.0712945974788894 2213 5 -1.9045670640908874 2213 6 -0.6654171269256359 2213 7 -0.1787756825431117 2213 8 -0.014845676583881584 2213 9 -0.645487566980492 2213 10 -0.5847008658489088 2213 11 -0.4387022091636936 2213 12 -0.6595563153224688 2213 13 -0.582038985747424 2213 14 -8.013530428121882 2213 15 -3.6204769771518954 2213 16 -2.2852566149213764 2213 17 -1.3521954581714013 2213 18 -5.385638454154911 2213 19 -3.39362881297809 2213 20 -5.218741756501345 2213 21 -2.4287220301650656 2213 22 -1.9339936009270247 2213 23 -4.427248226589015 2213 24 -2.773176141648481 2213 25 -2.0265214183901534 2214 1 0.32672637072654 2214 2 0.11396552237198776 2214 3 -1.6786162273417595 2214 4 -2.0279661867591763 2214 5 -0.5501497422822617 2214 6 -0.8085327069977382 2214 7 -0.5638319306428379 2214 8 -0.7765253013069895 2214 9 -6.179318952985788 2214 10 -1.9282095310967087 2214 11 -1.1791291735037035 2214 12 -1.1462804607422612 2214 13 -1.6136182760223647 2214 14 -2.6724258804955987 2214 15 -2.2488073424387807 2214 16 -1.614961692452539 2214 17 -1.7517046446371083 2214 18 -1.2536725939281794 2214 19 -4.594586550892348 2214 20 -2.477248467181969 2214 21 -4.445655097098133 2214 22 -5.998572500288535 2214 23 -6.616904222766749 2214 24 -6.069540646708635 2214 25 -4.002321705060005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23765.0 3 15.0 4 438.0 5 2970.0 6 3518.0 7 2816.0 8 2136.0 9 1766.0 10 1550.0 11 1395.0 12 1385.0 13 1324.0 14 1338.0 15 1450.0 16 1526.0 17 1506.0 18 1665.0 19 1971.0 20 2518.0 21 3375.0 22 4213.0 23 5207.0 24 6199.0 25 7318.0 26 8272.0 27 9272.0 28 10969.0 29 12865.0 30 15870.0 31 19970.0 32 25509.0 33 32587.0 34 42490.0 35 55930.0 36 75246.0 37 110424.0 38 177963.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.532444780019645 19.04082842505449 8.772817229164762 30.653909565761104 2 13.79807876531638 21.17322651292073 38.68304933369883 26.34564538806406 3 18.802550887313213 23.866743108787063 28.539194026903107 28.79151197699662 4 12.105973695225407 16.904148514092686 36.9295572049261 34.060320585755804 5 13.099234571321624 38.30208643419877 35.24538222802524 13.35329676645436 6 32.62480585755492 37.83285259965979 16.929073293395458 12.613268249389838 7 28.889226790121995 31.375408926103955 20.969642034650953 18.765722249123094 8 25.33976669230758 38.36997621448599 19.199088148140493 17.091168945065945 9 25.96371346393494 15.708809997696655 19.41217465257886 38.915301885789546 10 14.197575434289615 30.298819545648115 33.90162929519021 21.601975724872062 11 34.995800857509096 22.767897924002888 23.604337640523934 18.63196357796408 12 23.30044732049718 24.996316530820323 31.29829028094556 20.404945867736934 13 29.25341556522392 22.06175937153305 25.197464091075844 23.487360972167178 14 20.326531334358474 22.562330361605294 27.112509417188452 29.998628886847783 15 22.56127747098934 31.709728369450552 22.60695195044761 23.1220422091125 16 21.805015322960866 28.885107260726073 27.62611975483263 21.683757661480435 17 21.347261717080933 29.40740522461729 27.636840476786844 21.60849258151493 18 21.319656346553078 28.269783573766 29.467649515917767 20.942910563763157 19 23.11039080629121 27.429371247559764 27.114516188441563 22.345721757707466 20 23.13301224203546 28.025259051135436 27.86510629457794 20.97662241225117 21 22.266405984108577 27.197225410361398 27.100427120611847 23.435941484918178 22 22.519378074671703 28.71417395109403 27.038399601609477 21.728048372624794 23 21.730847714337493 28.433355777178292 27.54537511915619 22.290421389328024 24 22.72276563989457 27.41373950427932 28.31321484716286 21.55028000866325 25 22.122018885243197 28.09964477827004 27.818531936805595 21.959804399681172 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 128.0 1 128.0 2 140.5 3 153.0 4 153.0 5 153.0 6 263.0 7 373.0 8 373.0 9 373.0 10 674.0 11 975.0 12 975.0 13 975.0 14 1595.0 15 2215.0 16 2215.0 17 2215.0 18 4082.5 19 5950.0 20 5950.0 21 5950.0 22 9791.5 23 13633.0 24 13633.0 25 13633.0 26 20410.5 27 27188.0 28 27188.0 29 27188.0 30 37096.5 31 47005.0 32 47005.0 33 47005.0 34 55842.5 35 64680.0 36 64680.0 37 64680.0 38 71568.5 39 78457.0 40 78457.0 41 78457.0 42 83994.5 43 89532.0 44 89532.0 45 89532.0 46 92298.5 47 95065.0 48 95065.0 49 95065.0 50 90936.5 51 86808.0 52 86808.0 53 86808.0 54 76855.5 55 66903.0 56 66903.0 57 66903.0 58 56627.5 59 46352.0 60 46352.0 61 46352.0 62 37401.0 63 28450.0 64 28450.0 65 28450.0 66 21670.0 67 14890.0 68 14890.0 69 14890.0 70 10644.0 71 6398.0 72 6398.0 73 6398.0 74 4404.0 75 2410.0 76 2410.0 77 2410.0 78 1621.0 79 832.0 80 832.0 81 832.0 82 542.0 83 252.0 84 252.0 85 252.0 86 160.5 87 69.0 88 69.0 89 69.0 90 39.5 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09208360897026951 2 0.030792758839658128 3 0.032708097906239735 4 0.03285543168059216 5 0.023131402573331704 6 0.39500184903886815 7 0.19845859405272487 8 0.2103926297752718 9 0.21437064168278744 10 0.013849374789128536 11 0.0030940092614010553 12 0.0033886768101059183 13 0.0 14 0.06630019845859406 15 0.0022100066152864685 16 0.0016206715178767434 17 0.0030940092614010553 18 0.003536010584458349 19 8.840026461145872E-4 20 5.893350974097249E-4 21 0.0 22 0.0 23 0.0 24 2.9466754870486245E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 678731.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.5966336255503 #Duplication Level Percentage of deduplicated Percentage of total 1 92.4487254097567 79.13299678048047 2 5.853008570159058 10.01995660374222 3 0.9127436905445088 2.3438336182071295 4 0.28537965480905003 0.9771015102750509 5 0.13964300560778795 0.5976485594690246 6 0.09387973776355872 0.4821473710926045 7 0.047235306992424606 0.2830228286774675 8 0.03503148776087954 0.23988619385807644 9 0.02757335035895404 0.21241673716538928 >10 0.13416309551567854 2.119094611212596 >50 0.011222182931721421 0.670333985240458 >100 0.009495423169161027 1.5710226169553734 >500 0.0015537965158456222 0.9776959480531002 >1k 3.452881146323605E-4 0.3728426355710356 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 1265 0.1863772245558255 No Hit GTCCTACAGTGGACATTTCTAAATT 1258 0.1853458881353585 No Hit GTCCTACAGTGTGCATTTCTCATTT 933 0.13746241147081834 No Hit GTATCAACGCAGAGTACTTTTTTTT 899 0.1324530631428357 No Hit CTGTAGGACCTGGAATATGGCGAGA 875 0.12891705255837732 No Hit GTCCTAAAGTGTGTATTTCTCATTT 844 0.12434970555345196 No Hit CTTTAGGACGTGAAATATGGCGAGG 807 0.118898355902412 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACACCT 55 1.0875292E-5 15.603195 5 TAGGACT 110 1.0913936E-11 14.736351 4 GGCGAGG 225 0.0 14.31971 19 TAAGTAG 55 1.896957E-4 13.869507 5 TCTAAAC 50 0.0014551121 13.351378 3 TGGCGAG 665 0.0 12.825039 18 TAGCACC 60 3.9608523E-4 12.713715 4 GTGCTAT 60 3.963331E-4 12.712771 1 TAGGACC 365 0.0 12.539553 4 TGTAGGA 795 0.0 12.118503 2 GTAGGAC 805 0.0 11.846832 3 CTGTAGG 815 0.0 11.815858 1 CACTGTT 170 1.8189894E-12 11.735385 7 TATGGCG 760 0.0 11.72066 16 GACGTGG 300 0.0 11.716758 7 GATATAC 180 0.0 11.653374 1 ATGGCGA 765 0.0 11.520182 17 AGGACGT 580 0.0 11.508103 5 GTCCTAC 640 0.0 11.47129 1 CTATTCT 75 2.000413E-4 11.442342 4 >>END_MODULE