FastQCFastQC Report
Thu 26 May 2016
SRR1926440_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926440_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825852
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA1650.016.1882531
TGTAGGA5400.015.4706692
GTCCTAC4650.015.2221791
TCTTATA656.8504194E-414.7488352
CTGTAGG5300.014.36325551
TAGGACC2200.014.157724
GTAGGAC5250.014.0044293
GTGTAGG1253.08537E-813.8895211
TGCTATA700.00120003113.6953462
GACAGCT1057.2614057E-913.6094667
GTTTTAC600.00809917213.3553091
GTCTAGG700.001450723513.3553081
TCCTACA5150.013.2964532
AGGACGT3350.013.09915
GGACGTG3300.012.6601136
TTAGGAC2050.012.3118653
TAGGACG3500.012.2746744
GCTACAC1101.0663916E-611.9712223
GGACCTG3350.011.8475996
AGGACCT3650.011.7362135
Produced by FastQC (version 0.11.5)