##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926440_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 825852 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.74769208042119 27.0 10.0 31.0 2.0 31.0 2 16.708794069639595 22.0 2.0 31.0 2.0 31.0 3 20.432054411686355 25.0 15.0 31.0 2.0 31.0 4 22.663231426454136 28.0 7.0 35.0 2.0 37.0 5 23.253687101320818 30.0 7.0 35.0 2.0 37.0 6 23.9848641160886 31.0 7.0 35.0 2.0 37.0 7 23.843677801833742 31.0 10.0 35.0 2.0 37.0 8 24.11292338094477 31.0 13.0 35.0 2.0 37.0 9 26.949291156284662 32.0 17.0 38.0 2.0 39.0 10 27.695842596494288 32.0 25.0 38.0 2.0 39.0 11 24.716176748376224 30.0 10.0 37.0 2.0 39.0 12 25.91451373853911 31.0 17.0 37.0 2.0 39.0 13 22.459209398294124 29.0 2.0 37.0 2.0 39.0 14 25.942601095595823 31.0 17.0 38.0 2.0 40.0 15 27.06757385100478 31.0 18.0 38.0 2.0 40.0 16 27.43466747068482 32.0 22.0 39.0 2.0 40.0 17 26.778097044022417 31.0 17.0 39.0 2.0 40.0 18 26.86628596891453 31.0 17.0 38.0 2.0 40.0 19 27.263252979952824 31.0 22.0 39.0 2.0 40.0 20 27.521090946077507 32.0 24.0 39.0 2.0 40.0 21 25.34322856879925 31.0 10.0 38.0 2.0 40.0 22 26.308136324668343 31.0 16.0 39.0 2.0 40.0 23 26.324056852801714 31.0 10.0 39.0 2.0 40.0 24 25.322056494383983 31.0 2.0 39.0 2.0 40.0 25 25.127166853140757 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.02653596236383 1101 2 10.877665537989245 1101 3 7.26922204087651 1101 4 8.84355311210398 1101 5 8.572454835945326 1101 6 7.916225206885887 1101 7 7.750638457545879 1101 8 6.236672324285298 1101 9 6.2374555617582566 1101 10 5.907238892446671 1101 11 8.830336454834129 1101 12 6.31126877193957 1101 13 10.814754737874313 1101 14 8.317731135191789 1101 15 7.474492997456505 1101 16 6.87891513428092 1101 17 2.8746412578219847 1101 18 5.350028978510995 1101 19 6.088027217817423 1101 20 4.008897134095278 1101 21 1.328423955331207 1101 22 5.17250145616698 1101 23 6.391019750096174 1101 24 7.672813369092598 1101 25 7.9554151104978 1102 1 6.544748642971488 1102 2 11.561995912266504 1102 3 7.62144083769212 1102 4 9.026408910739598 1102 5 8.672082663453402 1102 6 7.547554553541165 1102 7 7.570240532599428 1102 8 7.82768669861208 1102 9 6.650903295418448 1102 10 6.110920502965556 1102 11 8.900432159869478 1102 12 6.217457037239377 1102 13 10.880716678717956 1102 14 8.248155431642164 1102 15 6.00852560897825 1102 16 6.562167867383973 1102 17 7.391145362119428 1102 18 7.413539979018616 1102 19 6.274261045152169 1102 20 6.623127346181597 1102 21 5.642874796619324 1102 22 6.6657293034846425 1102 23 7.085359905353759 1102 24 8.63444468803922 1102 25 8.415107319142205 1103 1 5.377435984696888 1103 2 9.674664967255481 1103 3 6.653153652082743 1103 4 8.207164616111523 1103 5 7.742177161561344 1103 6 7.20446163955171 1103 7 7.026817519061051 1103 8 6.762763294284554 1103 9 5.849231536348196 1103 10 5.101880446827671 1103 11 6.055547005964431 1103 12 4.769079635732719 1103 13 9.06123182167292 1103 14 7.191009380309534 1103 15 6.1675593266458115 1103 16 5.8399176405465845 1103 17 3.768271869003403 1103 18 2.610720807092349 1103 19 4.55185560065571 1103 20 5.005980436410475 1103 21 7.039557730362848 1103 22 2.7568315884283265 1103 23 5.064966001864207 1103 24 6.438986226979889 1103 25 2.5518810277662176 1104 1 6.075998868776619 1104 2 10.82477768696236 1104 3 7.329651955252896 1104 4 8.01383752621092 1104 5 8.005312748645164 1104 6 7.508738230058153 1104 7 7.464030470000221 1104 8 7.572961169264641 1104 9 6.309621300336783 1104 10 5.193870830653964 1104 11 8.3295664410625 1104 12 5.115812212452116 1104 13 9.79950535161002 1104 14 7.41257336020826 1104 15 6.677482040028803 1104 16 6.218269663949808 1104 17 6.61154802987156 1104 18 4.332980897557757 1104 19 5.046070448761679 1104 20 4.310043173254609 1104 21 3.2916429242552248 1104 22 5.53596951197202 1104 23 6.0003030265466855 1104 24 6.889087843674957 1104 25 6.0250947703984 1105 1 3.656284119163594 1105 2 9.658886551688543 1105 3 6.504693729807574 1105 4 5.932161526343464 1105 5 2.4097524489097744 1105 6 5.180086802622803 1105 7 6.0885030218693075 1105 8 3.210498150987977 1105 9 4.106774003493662 1105 10 3.5761461119517435 1105 11 6.931104880753296 1105 12 3.934086982514554 1105 13 5.168693954848791 1105 14 3.8915009967388627 1105 15 4.377725350182981 1105 16 4.713704048313577 1105 17 5.3045938091031 1105 18 2.8480424588136906 1105 19 2.853094254340771 1105 20 1.9181211650464434 1105 21 2.864155030592176 1105 22 4.276578639416364 1105 23 1.8708255753388912 1105 24 4.971119814818561 1105 25 5.65552558084843 1106 1 6.396866605797378 1106 2 11.290647994129596 1106 3 7.703532783812548 1106 4 9.008645830331027 1106 5 8.532219172158992 1106 6 7.449545209742418 1106 7 7.47431290825882 1106 8 7.282389670568236 1106 9 6.2341553165727 1106 10 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2204 6 4.725113272119394 2204 7 5.664184293967921 2204 8 5.759237187146674 2204 9 4.08369475128136 2204 10 3.518513910978914 2204 11 5.220620615941122 2204 12 5.3470384778946 2204 13 3.3080065293247713 2204 14 4.668440302476199 2204 15 4.892417385892383 2204 16 4.886295910164293 2204 17 -0.3203031454396914 2204 18 3.4707134361339236 2204 19 4.190797170869068 2204 20 4.520060959150875 2204 21 5.898572213300074 2204 22 5.384166887350574 2204 23 4.366231017701807 2204 24 5.910063183770134 2204 25 5.842291809287023 2205 1 5.734027716271761 2205 2 10.317214751622785 2205 3 7.038121605865275 2205 4 7.615642122094897 2205 5 7.810412673318048 2205 6 7.3495078685809965 2205 7 7.20362091368623 2205 8 7.039967892907125 2205 9 5.9162886799761445 2205 10 5.205133629288774 2205 11 7.474239605080317 2205 12 6.636459746651557 2205 13 9.624433976843672 2205 14 7.02313961552068 2205 15 5.774974856053948 2205 16 5.527052712559044 2205 17 6.399069767788998 2205 18 6.169784702972009 2205 19 4.78937145033332 2205 20 5.278425314218609 2205 21 -1.3424551497885417 2205 22 3.522734258882217 2205 23 4.835515662902715 2205 24 6.845909597401789 2205 25 7.1700348341939595 2206 1 4.220661110964283 2206 2 8.913166816853757 2206 3 6.019103520756907 2206 4 1.9534230641915933 2206 5 5.803955970712426 2206 6 6.01501262718201 2206 7 6.162210920530857 2206 8 6.164733133392254 2206 9 4.8450192348012315 2206 10 3.725667510192263 2206 11 3.461092605992313 2206 12 4.896957017193362 2206 13 8.129192577854141 2206 14 0.1699895018476525 2206 15 2.4667866679777752 2206 16 3.5317138662237184 2206 17 4.412979094199375 2206 18 -0.8296273491665147 2206 19 1.0534481623759469 2206 20 2.747337279633662 2206 21 3.622335119278034 2206 22 -2.095169569446334 2206 23 -2.7773306948045793 2206 24 1.3690200047783208 2206 25 2.9922204709214455 2207 1 2.110044734293652 2207 2 -14.209352005870404 2207 3 -6.193234099474369 2207 4 -3.4224118001767216 2207 5 1.8524548359453235 2207 6 2.420084856008696 2207 7 3.657305124212545 2207 8 3.7693039032326645 2207 9 1.2542976670214117 2207 10 0.8525020503414069 2207 11 -0.9296635451658695 2207 12 -1.7455733333235877 2207 13 1.1277371940146672 2207 14 -5.237356584106454 2207 15 -1.1556824411399873 2207 16 0.19119583603530899 2207 17 1.2577991525588281 2207 18 1.3682745925460829 2207 19 0.8283780950104074 2207 20 0.6481953797093141 2207 21 -3.646312886774055 2207 22 -3.998024859622493 2207 23 -1.2538925306055795 2207 24 0.5752695094434728 2207 25 1.1076958122521816 2208 1 5.132093967323872 2208 2 6.909757941773574 2208 3 6.384431005696939 2208 4 8.385193600760175 2208 5 4.021425626796798 2208 6 6.711779078475857 2208 7 7.152573793267383 2208 8 6.979298392072565 2208 9 5.521886993741351 2208 10 4.75552629944584 2208 11 6.837409569558112 2208 12 6.307253432543963 2208 13 9.20258104447852 2208 14 3.617767416995779 2208 15 4.7321342209340465 2208 16 5.2367014849744145 2208 17 5.94578895691264 2208 18 0.9638629088866715 2208 19 3.5516223312554693 2208 20 4.601459823541408 2208 21 7.337470524634046 2208 22 6.248000675419302 2208 23 1.8726427867453879 2208 24 3.5205675713521707 2208 25 5.107039984200377 2209 1 3.071213004298233 2209 2 4.704550605279913 2209 3 5.119676546937004 2209 4 0.3940871041058642 2209 5 4.8421602922466285 2209 6 5.662586310774756 2209 7 6.2194995304822775 2209 8 4.453657058527394 2209 9 4.329802377987253 2209 10 3.6402077295004887 2209 11 -0.014881883884932279 2209 12 3.431289076759491 2209 13 2.1813518233897966 2209 14 3.6828766737028538 2209 15 4.209654755061525 2209 16 3.723776900552636 2209 17 1.3452738024863997 2209 18 3.3980142203735895 2209 19 3.9644515885537217 2209 20 3.8997681365838552 2209 21 6.318245297677901 2209 22 4.978361625291274 2209 23 0.6337014720439527 2209 24 -2.5553929972113067 2209 25 0.6705766204830645 2210 1 -2.044656625848276 2210 2 -14.209352005870404 2210 3 -7.788787026736342 2210 4 -2.1547989478211136 2210 5 -0.5597982687264675 2210 6 -0.9308317613774655 2210 7 -0.9308360151527211 2210 8 -21.571748728346282 2210 9 -13.41956789576195 2210 10 -7.226701576703736 2210 11 -10.277781029888914 2210 12 -4.801634835393369 2210 13 -5.459765270010564 2210 14 -4.052960763093246 2210 15 -5.687194367022109 2210 16 -5.1243413214651845 2210 17 -4.676504021105671 2210 18 -4.761061106251475 2210 19 -10.247703119261228 2210 20 -7.232790230383333 2210 21 -5.329261832171255 2210 22 -10.176436056153147 2210 23 -8.03489785290008 2210 24 -10.48441115288651 2210 25 -8.143035510436562 2211 1 -0.47688509026774994 2211 2 4.482790851272455 2211 3 0.194610512054453 2211 4 0.9487912073420759 2211 5 0.3544473171483311 2211 6 -0.6729853194299018 2211 7 -21.29637908631377 2211 8 -13.859605871203426 2211 9 -9.895188548517435 2211 10 -8.079866370711226 2211 11 -10.613936728123264 2211 12 -5.785899147859926 2211 13 -7.628039748341223 2211 14 -5.822958087865853 2211 15 -6.217461889761541 2211 16 -12.755796645167699 2211 17 -8.93900535871937 2211 18 -6.879356986401287 2211 19 -9.731496591706385 2211 20 -7.690200610265624 2211 21 -5.325109879255258 2211 22 -6.753338142830515 2211 23 -8.861837392760968 2211 24 -22.906133999328457 2211 25 -22.717567345642554 2212 1 -1.3762121524964037 2212 2 -14.209352005870404 2212 3 -8.07791584725917 2212 4 -20.169780221229352 2212 5 -20.699124111423096 2212 6 -21.4079853194299 2212 7 -10.253687335228387 2212 8 -9.93137246206553 2212 9 -5.337455449489109 2212 10 -6.035821508193138 2212 11 -6.064923910260951 2212 12 -3.3337242460612906 2212 13 -6.447625235213252 2212 14 -4.192789594998374 2212 15 -4.2983777466976605 2212 16 -4.424605824411032 2212 17 -4.468239388076409 2212 18 -3.5648779704823284 2212 19 -4.20182324045561 2212 20 -4.439487358249913 2212 21 -2.3865901356125043 2212 22 -3.645256687382922 2212 23 -3.889385459744638 2212 24 -2.706423434929036 2212 25 -3.213225811633869 2213 1 -19.26188509026775 2213 2 -14.209352005870404 2213 3 -8.542144748189179 2213 4 -5.51551665533788 2213 5 -20.699124111423096 2213 6 -10.673876792298119 2213 7 -21.29637908631377 2213 8 -10.683376635323027 2213 9 -8.11462930156284 2213 10 -6.861533037377892 2213 11 -6.665404059241759 2213 12 -4.776009889831133 2213 13 -19.86875403405551 2213 14 -12.550339040246804 2213 15 -6.935413163294211 2213 16 -6.014124441402473 2213 17 -4.522388525939746 2213 18 -4.611178691827643 2213 19 -5.487011541872498 2213 20 -5.550001274717452 2213 21 -3.6184875093770756 2213 22 -4.6319538681904255 2213 23 -4.349600404942585 2213 24 -3.428614619483497 2213 25 -4.467954942541777 2214 1 -19.26188509026775 2214 2 -14.209352005870404 2214 3 -12.308091785647843 2214 4 -20.169780221229352 2214 5 -20.699124111423096 2214 6 -21.4079853194299 2214 7 -16.660015449950134 2214 8 -15.118077399674954 2214 9 -13.768075661404547 2214 10 -13.780880356725213 2214 11 -14.60547019465673 2214 12 -12.302222824183604 2214 13 -19.86875403405551 2214 14 -17.110420625328388 2214 15 -24.61603331833297 2214 16 -18.624742699113753 2214 17 -24.361148215862226 2214 18 -18.144986357030657 2214 19 -15.12154154675134 2214 20 -13.700090720155734 2214 21 -11.135289699435079 2214 22 -12.281390090882462 2214 23 -12.189259970183546 2214 24 -11.016273859468317 2214 25 -10.774385527460735 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 135514.0 3 4.0 4 608.0 5 2297.0 6 3202.0 7 3191.0 8 2727.0 9 2691.0 10 2465.0 11 2291.0 12 2224.0 13 2446.0 14 3820.0 15 8414.0 16 12572.0 17 13316.0 18 13519.0 19 15791.0 20 18340.0 21 21769.0 22 22411.0 23 24567.0 24 25413.0 25 25969.0 26 22208.0 27 16868.0 28 17833.0 29 20689.0 30 20469.0 31 23373.0 32 30477.0 33 38960.0 34 47953.0 35 55881.0 36 56711.0 37 60500.0 38 48369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.76053974680856 18.229867424480574 11.812340462987413 26.19725236572345 2 14.627596119158554 20.03485351407788 40.50108129878013 24.83646906798344 3 19.265242876122 23.524922330521214 28.650080046460353 28.55975474689643 4 11.569148562583052 16.68165000238797 38.00466918199415 33.744532253034826 5 12.526714277289283 38.19601048070253 35.96745702546878 13.309818216539401 6 32.19630923829112 37.1750656522884 18.022958729797242 12.605666379623237 7 28.075517091589536 30.761252380793263 22.63200454439135 18.53122598322585 8 24.77098385806638 37.59169169037909 20.204429524920993 17.432894926633537 9 26.026681536885622 15.476382415157925 19.77052181133814 38.72641423661832 10 13.776733769211008 29.605632729422727 35.281753246034874 21.33588025533139 11 33.7392486625484 22.241469930952416 24.642136118710958 19.377145287788228 12 23.01822490316976 24.65977696275602 32.10189085619264 20.22010727788158 13 28.147753558783258 21.26483369184136 25.735279216474193 24.852133532901195 14 20.11574128216523 22.026420329694 27.778892542307364 30.0789458458334 15 22.348499975041182 31.416163330504666 23.390405830379873 22.84493086407428 16 21.768493403865268 28.315550493773507 27.79467813559915 22.121277966762072 17 21.207270622199875 28.603528015899656 28.565101362773802 21.624099999126667 18 21.17968303167838 27.718676777181233 30.21727766400374 20.884362527136645 19 22.736621087867547 27.181445253195292 27.416120954487173 22.665812704449987 20 22.84261493565401 27.70794193619685 28.36810012642102 21.08134300172812 21 21.92635185755629 26.637206395566253 26.987966163788457 24.448475583088992 22 22.120417075521992 28.40351281107375 27.913009540436356 21.563060572967903 23 21.766279674485308 27.891682597716837 28.19365530016038 22.148382427637472 24 22.310052871915413 27.302250893990543 28.48155756578289 21.906138668311158 25 22.237497060199836 27.462219021779976 28.289242392282084 22.0110415257381 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 685.0 1 685.0 2 1619.0 3 2553.0 4 2553.0 5 2553.0 6 3709.0 7 4865.0 8 4865.0 9 4865.0 10 5689.5 11 6514.0 12 6514.0 13 6514.0 14 7967.0 15 9420.0 16 9420.0 17 9420.0 18 13232.0 19 17044.0 20 17044.0 21 17044.0 22 23895.5 23 30747.0 24 30747.0 25 30747.0 26 40785.5 27 50824.0 28 50824.0 29 50824.0 30 61537.5 31 72251.0 32 72251.0 33 72251.0 34 80802.0 35 89353.0 36 89353.0 37 89353.0 38 94929.5 39 100506.0 40 100506.0 41 100506.0 42 102264.0 43 104022.0 44 104022.0 45 104022.0 46 102436.5 47 100851.0 48 100851.0 49 100851.0 50 93259.5 51 85668.0 52 85668.0 53 85668.0 54 74418.0 55 63168.0 56 63168.0 57 63168.0 58 52579.0 59 41990.0 60 41990.0 61 41990.0 62 33254.0 63 24518.0 64 24518.0 65 24518.0 66 18554.0 67 12590.0 68 12590.0 69 12590.0 70 8954.0 71 5318.0 72 5318.0 73 5318.0 74 3681.5 75 2045.0 76 2045.0 77 2045.0 78 1365.0 79 685.0 80 685.0 81 685.0 82 436.5 83 188.0 84 188.0 85 188.0 86 114.0 87 40.0 88 40.0 89 40.0 90 22.5 91 5.0 92 5.0 93 5.0 94 3.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.332776332805393 2 36.56163574102866 3 5.758174588182871 4 13.797871773634984 5 13.765904786814101 6 14.928461758281145 7 9.405680436688414 8 7.6577885625995945 9 0.11345858579987697 10 1.491913805379172 11 5.775005691092351 12 5.7741580815933125 13 16.349902888168824 14 1.6374604650712234 15 2.9705080329163094 16 3.0368637479839 17 2.945443009159026 18 1.5543947341654436 19 1.5001477262269753 20 0.29218310302572376 21 4.479374028276252 22 1.6498113463429283 23 0.11418510822762433 24 9.285319887824937 25 0.11697011086732247 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 825852.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.5386684574887 #Duplication Level Percentage of deduplicated Percentage of total 1 96.70367189701068 90.45532704196249 2 2.55663743742494 4.7828892325059 3 0.37983922925353064 1.0658896719688211 4 0.12276958364678206 0.45934813525601087 5 0.06790400853573612 0.3175825270679352 6 0.03727199666801348 0.20918237634467615 7 0.02933030888012338 0.19204626266655184 8 0.016176033116967657 0.12104676789483163 9 0.010692489692494457 0.09001451234982188 >10 0.06577230519184618 1.0787706383775315 >50 0.0058289924792760146 0.3854850828743446 >100 0.00370839057821347 0.6285923138560192 >500 3.973275213650363E-4 0.21382543687513267 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 1.2108707129122409E-4 0.0 0.0 8 0.0 0.0 1.2108707129122409E-4 0.0 0.0 9 0.0 0.0 1.2108707129122409E-4 0.0 0.0 10 0.0 0.0 1.2108707129122409E-4 0.0 0.0 11 0.0 0.0 1.2108707129122409E-4 0.0 0.0 12 0.0 0.0 1.2108707129122409E-4 0.0 0.0 13 0.0 0.0 1.2108707129122409E-4 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAA 165 0.0 16.188253 1 TGTAGGA 540 0.0 15.470669 2 GTCCTAC 465 0.0 15.222179 1 TCTTATA 65 6.8504194E-4 14.748835 2 CTGTAGG 530 0.0 14.3632555 1 TAGGACC 220 0.0 14.15772 4 GTAGGAC 525 0.0 14.004429 3 GTGTAGG 125 3.08537E-8 13.889521 1 TGCTATA 70 0.001200031 13.695346 2 GACAGCT 105 7.2614057E-9 13.609466 7 GTTTTAC 60 0.008099172 13.355309 1 GTCTAGG 70 0.0014507235 13.355308 1 TCCTACA 515 0.0 13.296453 2 AGGACGT 335 0.0 13.0991 5 GGACGTG 330 0.0 12.660113 6 TTAGGAC 205 0.0 12.311865 3 TAGGACG 350 0.0 12.274674 4 GCTACAC 110 1.0663916E-6 11.971222 3 GGACCTG 335 0.0 11.847599 6 AGGACCT 365 0.0 11.736213 5 >>END_MODULE