FastQCFastQC Report
Thu 26 May 2016
SRR1926440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825852
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA13520.16370972038573497No Hit
GTCCTACAGTGGACATTTCTAAATT13470.16310428502927884No Hit
GTCCTACAGTGTGCATTTCTCATTT11960.144820137264304No Hit
CTGTAGGACCTGGAATATGGCGAGA9230.11176336680179984No Hit
CCCATGTACTCTGCGTTGATACCAC9070.10982597366114026No Hit
GTCCTAAAGTGTGTATTTCTCATTT8990.10885727709081046No Hit
CTTTAGGACGTGAAATATGGCGAGG8840.1070409710214421No Hit
GTATCAACGCAGAGTACTTTTTTTT8660.10486140373820008No Hit
GTACATGGGAAGCAGTGGTATCAAC8360.10122879159946334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCG307.702599E-419.0058339
CTAGTAC504.3782074E-617.172013
TAGGACC3250.014.3825144
TCCAACG602.6381877E-514.20776818
GTTATAC551.8897923E-413.8763713
TGTAGGA7650.013.3467922
TCTAGAC905.1265124E-712.7200073
GCTCTAA603.9540936E-412.7169041
GTAGGAC7450.012.6773223
CCAACGA604.1942E-412.62836319
CTGTAGG8600.012.5320651
CTAGGTA853.7587706E-612.3451364
GGCGAGG2150.011.45363119
GGACGTG5850.011.4146956
GACCGTG752.0679938E-411.4021137
AGGACGT6000.011.2883185
TAGGACG6050.011.1950254
GTCCTAA3200.011.0279411
TGGCGAG6050.010.95916118
GTCCTAC7950.010.9173421
Produced by FastQC (version 0.11.5)