FastQCFastQC Report
Thu 26 May 2016
SRR1926439_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926439_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824888
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12620.1529904665845545No Hit
CTGTAGGACGTGGAATATGGCAAGA9480.11492469280678104No Hit
GTCCTACAGTGTGCATTTCTCATTT9000.10910572101909594No Hit
GTCCTAAAGTGTGTATTTCTCATTT8430.10219569202121984No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTGG551.0796004E-519.3824271
TATAGAG551.9676214E-417.3748952
TGTAGGA8100.015.8427172
TAGGACC2750.015.556844
GATATAC1507.2759576E-1215.1021431
CTGTAGG7450.015.0246341
GTCCTAC7200.014.4358721
GTCCTAA3700.014.4058591
GTCTAGT658.4302056E-414.3504511
GTATAGG852.8119108E-514.1092661
GTAGGAC8350.014.0014393
CTATAGG1059.426385E-713.9599641
GGTCTAC700.001475456413.325421
TCCTACA8600.013.1754822
TAGGACA3000.012.83439354
GTGTAGG1254.770918E-712.7924031
GACGTGG4200.012.5994567
AGGACCT5050.012.5739135
AGGACGT7300.012.4059035
TCCTAAA4200.012.35154152
Produced by FastQC (version 0.11.5)