FastQCFastQC Report
Thu 26 May 2016
SRR1926439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926439_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824888
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA26440.3205283626383218No Hit
GTCCTACAGTGGACATTTCTAAATT24270.2942217610148287No Hit
GTCCTACAGTGTGCATTTCTCATTT19320.23421361445432592No Hit
CTTTAGGACGTGAAATATGGCGAGG18510.22439409956260728No Hit
GTCCTAAAGTGTGTATTTCTCATTT18070.2190600420905626No Hit
CTGTAGGACCTGGAATATGGCGAGA15200.184267439943362No Hit
CTGAAGGACCTGGAATATGGCGAGA10630.12886597938144329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5950.014.2610394
TGTCCGC400.0053387714.22474710
TCCAACG1051.4206307E-913.53588118
GACTGAC500.001491794513.3079187
TGTAGGA14800.012.8217982
CTGTAGG14950.012.4926521
GTAGGAC14100.012.4431343
GGACCTG8200.012.2082546
AGGACCT8750.012.2036045
TAGGTTG550.00298520412.1342655
GTCCTAA5600.011.5707261
GGCGAGG5600.011.336319
TCAAGAC1104.725025E-711.2689073
GTATAGG1104.766207E-711.261351
GTCCTAC12650.011.2236861
GTATAAG855.1539362E-511.2103941
AGGACGT13100.011.2079855
ACGTTTT855.4595068E-511.14719619
CTTAGTC600.00571206911.12443353
GATATAC3350.011.093271
Produced by FastQC (version 0.11.5)