##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926439_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 824888 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.280502322739572 31.0 28.0 31.0 22.0 34.0 2 28.80848794018097 31.0 28.0 33.0 23.0 34.0 3 29.19380449224622 31.0 29.0 33.0 25.0 34.0 4 32.96587294275101 35.0 33.0 37.0 27.0 37.0 5 32.610594407968115 35.0 33.0 37.0 26.0 37.0 6 32.70056541009204 35.0 33.0 37.0 26.0 37.0 7 32.52584229616627 35.0 33.0 37.0 25.0 37.0 8 32.4938597724782 35.0 33.0 37.0 25.0 37.0 9 33.73559077111074 37.0 34.0 39.0 23.0 39.0 10 33.63132207039016 37.0 33.0 39.0 23.0 39.0 11 33.89526820610798 37.0 34.0 39.0 25.0 39.0 12 33.67720102607869 37.0 33.0 39.0 23.0 39.0 13 33.82930531175141 37.0 34.0 39.0 25.0 39.0 14 34.454375624327184 38.0 34.0 40.0 22.0 41.0 15 34.59837820407134 38.0 34.0 40.0 24.0 41.0 16 34.56733035272667 38.0 34.0 40.0 23.0 41.0 17 34.48546469338868 38.0 34.0 40.0 22.0 41.0 18 34.462756155987236 38.0 34.0 40.0 22.0 41.0 19 34.500567349749296 39.0 34.0 40.0 21.0 41.0 20 34.48241337005751 39.0 34.0 40.0 19.0 41.0 21 34.39168105245803 39.0 34.0 40.0 19.0 41.0 22 34.31738611787297 39.0 34.0 40.0 18.0 41.0 23 33.97239746486796 39.0 34.0 40.0 16.0 41.0 24 34.04999224137095 39.0 34.0 40.0 17.0 41.0 25 33.84074322817158 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6190119049970129 1101 2 0.7987646835997886 1101 3 0.7130764077504352 1101 4 0.47101515671869265 1101 5 0.4933411331219233 1101 6 0.156332623509158 1101 7 0.2701721521642284 1101 8 0.37572517794854576 1101 9 0.7749369188986535 1101 10 0.8797080614017432 1101 11 0.6663123524853916 1101 12 0.843039291126189 1101 13 0.6701013596348844 1101 14 1.002139800052511 1101 15 0.807914266054091 1101 16 0.7580370906023575 1101 17 0.578612890257638 1101 18 0.8475641095475268 1101 19 0.8335767452006095 1101 20 0.6482933053386191 1101 21 0.7353969889747063 1101 22 0.7556641114198186 1101 23 1.0043936115687728 1101 24 0.8966476256067892 1101 25 1.0582020204746811 1102 1 0.9524649876124904 1102 2 0.9056610895844344 1102 3 0.7715704100404004 1102 4 0.6808543212657341 1102 5 0.7423037329236664 1102 6 0.54057413027531 1102 7 0.7462024442500166 1102 8 0.8592554892454487 1102 9 1.0542367506092845 1102 10 1.1206634685621069 1102 11 0.7710936147324787 1102 12 0.8250961099192651 1102 13 0.8486513815355536 1102 14 1.2199352477867507 1102 15 0.9129635233523956 1102 16 0.8924812746294677 1102 17 1.1501070539209692 1102 18 1.0725262636514046 1102 19 1.1385493501509316 1102 20 1.0988073947701977 1102 21 1.0611444934508967 1102 22 1.0146270570216274 1102 23 1.2369182210068033 1102 24 1.2042953782905812 1102 25 1.2935218471135812 1103 1 0.12718953758145446 1103 2 0.13490922738704114 1103 3 0.0653708445531258 1103 4 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0.1570263922131474 2202 19 0.04647187027279642 2202 20 0.10653863059918933 2202 21 0.19600965945137716 2202 22 0.30931882049918613 2202 23 0.5109076477772589 2202 24 0.14836030586746318 2202 25 0.3082197659716428 2203 1 -0.12622560701519703 2203 2 0.11724370227288716 2203 3 -0.1599606062199257 2203 4 -0.5767069117318186 2203 5 -0.12268863434945843 2203 6 -0.3732384872868053 2203 7 -0.09915830782531998 2203 8 0.049869249098513535 2203 9 0.3591324702404961 2203 10 0.3119760559155509 2203 11 0.2420852247746481 2203 12 0.15546832115447984 2203 13 0.12178921770783546 2203 14 0.5046686863805547 2203 15 0.2805775007079703 2203 16 0.39522371943820644 2203 17 0.40421543461479814 2203 18 0.4147060415571744 2203 19 0.4106329033363352 2203 20 0.38831833635466495 2203 21 0.46675041918587823 2203 22 0.43587453459672076 2203 23 0.5290819883113471 2203 24 0.5199010824952239 2203 25 0.34923320114724277 2204 1 -0.1434252101746587 2204 2 -0.09255711430283853 2204 3 -0.2552148874184006 2204 4 -0.4314027242206464 2204 5 -0.2946665912231694 2204 6 -0.6903941086254193 2204 7 -0.3316208078002205 2204 8 -0.4158841755590217 2204 9 -0.10575336781401745 2204 10 -0.41457952485536254 2204 11 -0.37183522235378064 2204 12 -0.4782605138494276 2204 13 -0.5158854292828252 2204 14 -0.13612636034260106 2204 15 -0.12853326318998626 2204 16 -0.3598587916626812 2204 17 -0.4022419602555942 2204 18 -0.27263643222945433 2204 19 -0.28351068626306386 2204 20 -0.17335612587218208 2204 21 -0.1590551610829536 2204 22 -0.2874788081220814 2204 23 0.19151548840071797 2204 24 -0.12260001705029566 2204 25 -0.3254519472562549 2205 1 0.7354763212871838 2205 2 0.7187185069128041 2205 3 0.6751424663801515 2205 4 0.6456228712737584 2205 5 0.5622966254944899 2205 6 0.6691459955551409 2205 7 0.6657260936474145 2205 8 0.7311746479703913 2205 9 0.924555782470442 2205 10 0.9403850560408102 2205 11 0.7924044386592968 2205 12 0.7955811728544049 2205 13 0.6649568175725094 2205 14 1.2026414587627627 2205 15 1.0655487551845084 2205 16 0.891658761656501 2205 17 1.1355387347133288 2205 18 0.9682839690981382 2205 19 0.9438936783460292 2205 20 1.0955918562487028 2205 21 0.786828296919019 2205 22 1.0069798835621455 2205 23 1.265312591617203 2205 24 1.1193682867575916 2205 25 1.2053906806545314 2206 1 0.25346064416972425 2206 2 0.42965425226266163 2206 3 0.41921714336211124 2206 4 0.3646319794451216 2206 5 0.6036072963659791 2206 6 0.6029627963911537 2206 7 0.525090507741595 2206 8 0.4226504241696105 2206 9 0.7341343044854085 2206 10 0.7418345304694327 2206 11 0.5000074508338415 2206 12 0.6256312705811666 2206 13 0.49045190153585594 2206 14 0.6750255277227737 2206 15 0.7218586743116688 2206 16 0.6256674893153047 2206 17 0.7526725656209692 2206 18 0.5688877775648251 2206 19 0.39375882840718646 2206 20 0.6115743118354615 2206 21 0.7153708224458413 2206 22 0.6546187567252417 2206 23 0.9037551258679741 2206 24 0.641589292164582 2206 25 0.6847112151540315 2207 1 -1.0845461855718668 2207 2 -0.11668527913064963 2207 3 0.2171011362973836 2207 4 0.21716717042774292 2207 5 0.025124961973943982 2207 6 0.05803223083397313 2207 7 0.21547618726677342 2207 8 0.2510152657817599 2207 9 0.4976725047992332 2207 10 0.09761877582467804 2207 11 -0.007537670183722867 2207 12 0.04716314902541541 2207 13 -0.14436537824098394 2207 14 -5.449757489643417 2207 15 -2.383125862122462 2207 16 -1.287686323135894 2207 17 -0.8820158511069707 2207 18 -0.6139262503150746 2207 19 -0.3293277448211356 2207 20 -0.10021495159851668 2207 21 0.026926616355922306 2207 22 -0.1222444513582559 2207 23 0.22923665676099603 2207 24 -0.024056577016679626 2207 25 -0.10801446573557882 2208 1 0.23540032189517746 2208 2 0.7097049790210264 2208 3 0.7500930667753494 2208 4 0.6835161681273831 2208 5 0.7810684753196924 2208 6 0.6128456459579326 2208 7 0.5981778340334927 2208 8 0.8298674584279055 2208 9 0.9677842366428067 2208 10 0.9056837336513865 2208 11 0.8194450223346266 2208 12 0.8828132749975879 2208 13 0.5926813957758839 2208 14 1.0608113934032843 2208 15 0.7894733637876357 2208 16 0.8229857110409071 2208 17 0.7852423370845116 2208 18 1.0035628868667956 2208 19 0.38697013373438693 2208 20 0.7048485821948915 2208 21 0.8888802805031517 2208 22 0.8988175488608263 2208 23 1.191258784047669 2208 24 1.107998777713668 2208 25 1.071145354617144 2209 1 -0.32084662446211354 2209 2 -0.14755139790679905 2209 3 0.34217894673146887 2209 4 0.6559628618965689 2209 5 0.5834975616697946 2209 6 0.43644604051161906 2209 7 0.7593474828991447 2209 8 0.7152864873464821 2209 9 0.8840042648515478 2209 10 0.7138449106750855 2209 11 0.7886255859564102 2209 12 0.689461688947091 2209 13 0.9027148590906933 2209 14 1.165679706501912 2209 15 1.0381841504060034 2209 16 1.0277148241247858 2209 17 1.0602665357625725 2209 18 1.2197672435507059 2209 19 1.1133545285830593 2209 20 1.0031754164546882 2209 21 1.0443768373769586 2209 22 1.092119354773594 2209 23 1.220482490455474 2209 24 1.3449697485185368 2209 25 1.417966339710759 2210 1 -0.06922647883730448 2210 2 -0.3849959786602639 2210 3 0.385531577707642 2210 4 0.6258200538367475 2210 5 0.9410619728406218 2210 6 0.8696844848829244 2210 7 1.0236388645807963 2210 8 0.984993659115446 2210 9 1.2748800669280982 2210 10 1.2379972296423176 2210 11 1.0055445389518098 2210 12 0.8114361359927571 2210 13 0.8290584630622035 2210 14 1.0746884487365094 2210 15 1.1140077910674506 2210 16 1.1412782365368415 2210 17 1.0874254488076218 2210 18 0.9422201249388138 2210 19 0.9364798181250862 2210 20 1.2170851959332936 2210 21 1.1757084572749577 2210 22 1.2475388076642702 2210 23 1.4206949330500578 2210 24 1.2312551809821173 2210 25 1.2587767788034938 2211 1 -1.2372566091781394 2211 2 -0.35427756665285415 2211 3 0.2827214413647887 2211 4 0.5076478106906777 2211 5 0.9465254968690004 2211 6 0.7478862423454942 2211 7 0.7602504881371885 2211 8 0.7578721330206477 2211 9 0.9734078848043026 2211 10 0.9536487307533292 2211 11 0.6890931555605206 2211 12 0.8930314069511667 2211 13 0.5003039978087713 2211 14 1.0309844758542397 2211 15 0.8501521221931867 2211 16 1.1522532796400853 2211 17 1.069680467058923 2211 18 0.9958163515788101 2211 19 1.0429328312382822 2211 20 1.0855065122739163 2211 21 1.13726231536468 2211 22 1.0833150537027052 2211 23 1.3993054922767385 2211 24 1.1197568863885223 2211 25 1.1300551189554682 2212 1 1.1188433916199934 2212 2 0.12853622615702776 2212 3 0.446608031924999 2212 4 0.4840805267121411 2212 5 0.5075957405923432 2212 6 0.6246368815173469 2212 7 0.8233376150430658 2212 8 0.8025660089428257 2212 9 1.1175997347977855 2212 10 0.6566207136094206 2212 11 0.5919289236442253 2212 12 0.30051098138641663 2212 13 0.40853408371452815 2212 14 0.45562604743217605 2212 15 0.360635604821816 2212 16 -0.3745397936259067 2212 17 0.3787666840926889 2212 18 0.5107789590275189 2212 19 0.5946420339828009 2212 20 0.5033580967018025 2212 21 0.7417563386754935 2212 22 0.6558539427541703 2212 23 0.8788501305493668 2212 24 0.969697159403367 2212 25 0.1998263473094184 2213 1 0.5391905146892135 2213 2 0.5239691101341108 2213 3 -0.07341335421850914 2213 4 -0.8853379061656526 2213 5 -1.5710811617545666 2213 6 -0.15549934698061207 2213 7 0.10120604699371682 2213 8 0.15446758323494691 2213 9 -0.24360604811431585 2213 10 -0.1421239321284915 2213 11 -0.003926738429981924 2213 12 -0.2675365131588805 2213 13 -0.23656286474238897 2213 14 -7.47594756478636 2213 15 -2.895605625932202 2213 16 -1.615106711857905 2213 17 -0.9518155011261484 2213 18 -4.791467822234264 2213 19 -2.7168496186221702 2213 20 -4.849521306464091 2213 21 -1.624953594755496 2213 22 -1.324544358716281 2213 23 -4.259061444546283 2213 24 -1.8291247484137045 2213 25 -1.4704153902838328 2214 1 0.3233385274804448 2214 2 -1.286514008391748E-4 2214 3 -1.5161695594872775 2214 4 -1.819425008343206 2214 5 -0.4082244539732329 2214 6 -0.325205453269632 2214 7 -0.7214359508744117 2214 8 -0.7504296301044775 2214 9 -5.07676121790265 2214 10 -1.4570257137639402 2214 11 -0.7649043537794569 2214 12 -0.9881136412234426 2214 13 -1.2746091568087863 2214 14 -2.505272979739914 2214 15 -2.1771101180899635 2214 16 -1.4512388878895734 2214 17 -1.5086435809190561 2214 18 -0.7763361030321931 2214 19 -4.221912813383856 2214 20 -1.6997146976625075 2214 21 -3.7068951205663687 2214 22 -5.76137852957055 2214 23 -5.972662784579786 2214 24 -5.497515185447359 2214 25 -3.099888513475552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27425.0 3 12.0 4 536.0 5 3302.0 6 4062.0 7 3228.0 8 2393.0 9 1904.0 10 1772.0 11 1572.0 12 1541.0 13 1600.0 14 1554.0 15 1581.0 16 1597.0 17 1749.0 18 2013.0 19 2361.0 20 3089.0 21 3876.0 22 4938.0 23 6087.0 24 7396.0 25 8786.0 26 10071.0 27 11115.0 28 12879.0 29 15885.0 30 19027.0 31 24126.0 32 30659.0 33 39877.0 34 52153.0 35 68711.0 36 92698.0 37 135807.0 38 217506.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.188454225681085 19.071591420629172 8.865479280190932 30.874475073498807 2 13.253860939480115 21.014546358559553 40.12163399445795 25.60995870750238 3 18.742618080397744 23.829382162129388 28.8224095070636 28.605590250409264 4 11.93261882414003 17.069760447817302 37.53780520044335 33.45981552759931 5 12.994785982781618 38.50503213289681 35.57123802594883 12.92894385837274 6 32.611896706987615 37.97162119405165 17.094823180081292 12.32165891887945 7 28.953686737853033 31.012607472138654 21.224684205666073 18.80902158434224 8 24.88942031944161 39.37596605325323 19.28801267656294 16.446600950742223 9 26.269554153750057 15.403684742018237 19.312388884770698 39.01437221946101 10 14.473146570799814 30.194300125491814 34.22301168225715 21.109541621451218 11 35.06754389474233 22.166375105925653 24.44588305322893 18.320197946103086 12 23.94237110602475 24.53798444349614 31.761720720021724 19.757923730457385 13 30.040199396766592 22.06335890448158 25.106559920861983 22.789881777889846 14 20.109531736056176 23.15962903828515 26.92250763130948 29.80833159434919 15 22.230519775723597 33.12901954841643 22.37902712532202 22.26143355053796 16 21.558263332149306 28.497078923026383 28.80003685442623 21.144620890398087 17 20.9982481557141 29.827179651934603 28.09473294982694 21.079839242524354 18 20.905345902800004 28.094237054710153 30.63577157709952 20.36464546539032 19 23.79678885068786 26.97059921733691 26.692011890271992 22.540600041703236 20 23.539666375715257 28.185554262438174 27.81762195713316 20.457157404713413 21 22.23710641578786 27.07292029090045 26.66971354864363 24.020259744668056 22 22.75593777579502 29.498550106196237 26.645071815810144 21.1004403021986 23 21.721615540533985 28.91325852721824 27.614051847038628 21.751074085209144 24 23.353222998627686 27.14017971011682 28.467396627889496 21.039200663366 25 22.13209550872361 28.358759007283414 27.988042012976305 21.521103471016673 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 117.0 1 117.0 2 123.0 3 129.0 4 129.0 5 129.0 6 203.0 7 277.0 8 277.0 9 277.0 10 507.5 11 738.0 12 738.0 13 738.0 14 1393.0 15 2048.0 16 2048.0 17 2048.0 18 4168.0 19 6288.0 20 6288.0 21 6288.0 22 11113.5 23 15939.0 24 15939.0 25 15939.0 26 24901.5 27 33864.0 28 33864.0 29 33864.0 30 48127.0 31 62390.0 32 62390.0 33 62390.0 34 73148.0 35 83906.0 36 83906.0 37 83906.0 38 91414.5 39 98923.0 40 98923.0 41 98923.0 42 104574.5 43 110226.0 44 110226.0 45 110226.0 46 113358.0 47 116490.0 48 116490.0 49 116490.0 50 110208.0 51 103926.0 52 103926.0 53 103926.0 54 89992.0 55 76058.0 56 76058.0 57 76058.0 58 64734.0 59 53410.0 60 53410.0 61 53410.0 62 42624.5 63 31839.0 64 31839.0 65 31839.0 66 24345.0 67 16851.0 68 16851.0 69 16851.0 70 12107.5 71 7364.0 72 7364.0 73 7364.0 74 5068.5 75 2773.0 76 2773.0 77 2773.0 78 1870.5 79 968.0 80 968.0 81 968.0 82 619.5 83 271.0 84 271.0 85 271.0 86 173.5 87 76.0 88 76.0 89 76.0 90 45.5 91 15.0 92 15.0 93 15.0 94 8.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08789071970982727 2 0.034307687831560164 3 0.02885240178060537 4 0.03224680199008835 5 0.022790972835100037 6 0.3816275664090155 7 0.2283946426666408 8 0.23663818603252806 9 0.24015381482092113 10 0.016123400995044175 11 0.002060885841471812 12 0.002909485893842558 13 0.0 14 0.0790410334493895 15 0.0015759715258313856 16 0.002545800157112238 17 0.005212828893134583 18 0.003879314525123411 19 0.001454742946921279 20 9.698286312808527E-4 21 1.2122857891010659E-4 22 0.0 23 0.0 24 4.8491431564042637E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 824888.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.02735218565036 #Duplication Level Percentage of deduplicated Percentage of total 1 91.94443250154063 75.41958346313632 2 6.086395603102985 9.985018313538447 3 0.9900960845459877 2.4364488067406156 4 0.3437464879926967 1.1278645693262948 5 0.16177887714091402 0.6635146465718408 6 0.09671365921358477 0.47598992312854116 7 0.06456871391219424 0.37074804453750415 8 0.047615742426784284 0.31246346188984386 9 0.03301891878465617 0.243760903194454 >10 0.19503516415850206 2.891655421350555 >50 0.01941211866652199 1.0839574341782883 >100 0.014519315549950298 2.547362500538528 >500 0.0016297190339163196 0.832106804477909 >1k 0.0010370939306740216 1.6095257073908902 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2644 0.3205283626383218 No Hit GTCCTACAGTGGACATTTCTAAATT 2427 0.2942217610148287 No Hit GTCCTACAGTGTGCATTTCTCATTT 1932 0.23421361445432592 No Hit CTTTAGGACGTGAAATATGGCGAGG 1851 0.22439409956260728 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1807 0.2190600420905626 No Hit CTGTAGGACCTGGAATATGGCGAGA 1520 0.184267439943362 No Hit CTGAAGGACCTGGAATATGGCGAGA 1063 0.12886597938144329 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.2122857891010658E-4 0.0 11 0.0 0.0 0.0 1.2122857891010658E-4 0.0 12 0.0 0.0 0.0 2.4245715782021316E-4 0.0 13 0.0 0.0 0.0 2.4245715782021316E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 595 0.0 14.261039 4 TGTCCGC 40 0.00533877 14.224747 10 TCCAACG 105 1.4206307E-9 13.535881 18 GACTGAC 50 0.0014917945 13.307918 7 TGTAGGA 1480 0.0 12.821798 2 CTGTAGG 1495 0.0 12.492652 1 GTAGGAC 1410 0.0 12.443134 3 GGACCTG 820 0.0 12.208254 6 AGGACCT 875 0.0 12.203604 5 TAGGTTG 55 0.002985204 12.134265 5 GTCCTAA 560 0.0 11.570726 1 GGCGAGG 560 0.0 11.3363 19 TCAAGAC 110 4.725025E-7 11.268907 3 GTATAGG 110 4.766207E-7 11.26135 1 GTCCTAC 1265 0.0 11.223686 1 GTATAAG 85 5.1539362E-5 11.210394 1 AGGACGT 1310 0.0 11.207985 5 ACGTTTT 85 5.4595068E-5 11.147196 19 CTTAGTC 60 0.005712069 11.1244335 3 GATATAC 335 0.0 11.09327 1 >>END_MODULE