FastQCFastQC Report
Thu 26 May 2016
SRR1926438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732872
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9160.12498771954720606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGC250.0026535421.891423
TCCTATA509.355952E-519.1446482
GTCCTAT754.3198634E-717.7896771
TGTAGGA5800.016.5040072
GGACTGC400.002852617815.6291986
TAGGACC2800.015.3088044
CAATACT702.076773E-615.3088044
ATAATAC753.4121404E-614.5942823
GTCTATG550.00457896314.5551891
GTCTTAA550.00457896314.5551891
GTGCTAG658.347221E-414.3685841
GTAGGAC6200.014.1234983
GGACGTG5100.014.0969236
AGGACGT5400.014.0908455
GACGTGG2250.014.0328127
CTGTAGG5800.014.0323731
TAGGACG5900.013.9854984
CCTATAG755.512668E-513.1348543
ATAAAGC500.00904204213.1348523
CTAGGAC1003.4633013E-713.1348523
Produced by FastQC (version 0.11.5)