##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926438_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 732872 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.004934013033655 27.0 10.0 31.0 2.0 31.0 2 16.89059753954306 23.0 2.0 31.0 2.0 31.0 3 20.65418927179644 25.0 15.0 31.0 2.0 31.0 4 22.92733656081826 28.0 10.0 35.0 2.0 37.0 5 23.543206726413345 30.0 10.0 35.0 2.0 37.0 6 24.29026624021657 31.0 10.0 35.0 2.0 37.0 7 24.127233677913743 31.0 10.0 35.0 2.0 37.0 8 24.398143195537557 32.0 17.0 35.0 2.0 37.0 9 27.274623672346603 32.0 18.0 38.0 2.0 39.0 10 28.035846641705508 33.0 26.0 38.0 2.0 39.0 11 25.018920084271198 31.0 10.0 37.0 2.0 39.0 12 26.244375552620376 31.0 17.0 37.0 2.0 39.0 13 22.753160169852308 29.0 2.0 37.0 2.0 39.0 14 26.266023261906582 31.0 17.0 38.0 2.0 40.0 15 27.415633289305635 32.0 22.0 39.0 2.0 40.0 16 27.786831806918535 32.0 24.0 39.0 2.0 40.0 17 27.11447974543986 31.0 18.0 39.0 2.0 40.0 18 27.202144439956772 31.0 19.0 38.0 2.0 40.0 19 27.59610136558635 31.0 24.0 39.0 2.0 40.0 20 27.87241155344999 32.0 26.0 39.0 2.0 40.0 21 25.663594734141842 31.0 10.0 38.0 2.0 40.0 22 26.641286609394275 31.0 18.0 39.0 2.0 40.0 23 26.66121505528933 31.0 17.0 39.0 2.0 40.0 24 25.638051392330446 31.0 2.0 39.0 2.0 40.0 25 25.431918534205156 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.383142266583654 1101 2 11.162763563339968 1101 3 7.5476959421877226 1101 4 9.242471247794146 1101 5 8.946322769968301 1101 6 8.438756499398995 1101 7 7.884569046314915 1101 8 6.487243128675651 1101 9 6.6158573222033965 1101 10 6.125428580239454 1101 11 9.003278097726518 1101 12 6.5005731998465315 1101 13 11.195382212138352 1101 14 8.49679179380999 1101 15 7.650207784123129 1101 16 7.22242556394059 1101 17 2.7676109730767813 1101 18 5.541525955790277 1101 19 6.310801716959787 1101 20 4.265999579946566 1101 21 1.272465259187495 1101 22 5.249417000386192 1101 23 6.488507811607807 1101 24 7.779096522429786 1101 25 8.332602347732806 1102 1 6.028676243145831 1102 2 11.308309110226578 1102 3 7.316346087243055 1102 4 8.66882065210513 1102 5 8.622106446311008 1102 6 7.441808020226798 1102 7 7.576187016124894 1102 8 7.640242400354175 1102 9 6.500627243257249 1102 10 5.859722778269823 1102 11 8.557112990414804 1102 12 5.721844084282129 1102 13 10.604776828405502 1102 14 8.084524083297069 1102 15 6.052788088264325 1102 16 6.435398089900744 1102 17 7.350026394005187 1102 18 7.287797266500988 1102 19 6.176976299758245 1102 20 6.2928810973885305 1102 21 5.517874885210251 1102 22 6.555031896143657 1102 23 6.787090994932893 1102 24 8.341589671579793 1102 25 7.967643257964138 1103 1 5.463246604166219 1103 2 9.889820926737176 1103 3 6.854883628571123 1103 4 8.472096835249879 1103 5 7.8712880608934555 1103 6 7.334688051371042 1103 7 7.615828001998665 1103 8 7.1337333668256875 1103 9 5.916273954841564 1103 10 5.089449932816837 1103 11 5.843021163954653 1103 12 4.73431561767697 1103 13 9.103911116568987 1103 14 7.235374921116197 1103 15 6.445528184149854 1103 16 6.051370457561298 1103 17 3.611935513444486 1103 18 2.4894512069206414 1103 19 4.539008625117468 1103 20 5.069197888173051 1103 21 7.345570242692951 1103 22 2.93492184728267 1103 23 5.087551177534259 1103 24 6.5825330301895235 1103 25 2.5789049960166572 1104 1 6.068660409767702 1104 2 10.959123869476151 1104 3 7.187034057296469 1104 4 7.725448191432015 1104 5 7.787038690203673 1104 6 7.676944680850067 1104 7 7.6463327907142755 1104 8 7.524245801598369 1104 9 6.436552762851058 1104 10 5.426158826571399 1104 11 8.49886729450363 1104 12 5.6412838319254455 1104 13 10.009392942288539 1104 14 7.588555730505952 1104 15 6.858789500562562 1104 16 6.425425871615147 1104 17 6.971803519660629 1104 18 4.191889877102508 1104 19 5.279990994501468 1104 20 4.530073287232685 1104 21 3.802362572804018 1104 22 5.897884396497954 1104 23 5.907281728497214 1104 24 7.2892161309139105 1104 25 6.344712226009062 1105 1 3.6625414606244426 1105 2 9.956709411309149 1105 3 6.74443463277936 1105 4 5.901776293539395 1105 5 2.3456185732816905 1105 6 5.234621468906155 1105 7 6.3855064587692105 1105 8 3.146821736037513 1105 9 4.272265727548685 1105 10 3.8875437083764375 1105 11 7.284538947407704 1105 12 4.864023452337317 1105 13 5.935435224428872 1105 14 4.469685848244563 1105 15 4.935270960694279 1105 16 5.240703909593172 1105 17 5.918229689240743 1105 18 3.5050470828880833 1105 19 3.395620783186839 1105 20 1.9399889583331635 1105 21 3.320124242184775 1105 22 4.753433627185892 1105 23 2.6094705330011614 1105 24 5.4993142224051255 1105 25 6.238905997535628 1106 1 6.562777002626493 1106 2 11.415079080123352 1106 3 7.854892732904968 1106 4 9.088360088883874 1106 5 8.647337836107454 1106 6 7.627317728950164 1106 7 7.661943273790342 1106 8 7.174318666239916 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2.3683171356426485 1107 23 -0.4785373441503076 1107 24 2.8652650695006905 1107 25 3.6315765542559113 1108 1 5.317794063759521 1108 2 10.782763280171501 1108 3 0.2966765357088299 1108 4 6.039444176888765 1108 5 7.173296454178828 1108 6 7.265790120934902 1108 7 7.422100666793657 1108 8 7.3246690329269555 1108 9 5.8557364092682604 1108 10 4.923673407695471 1108 11 7.238343320863272 1108 12 2.810652109459152 1108 13 3.7470368598767827 1108 14 4.9411822264914065 1108 15 5.164486554039307 1108 16 4.870808200050234 1108 17 0.36288536332068233 1108 18 4.091568903007676 1108 19 4.889628738782633 1108 20 5.131369492353123 1108 21 5.005286372228348 1108 22 1.6738605743497956 1108 23 4.659312670778686 1108 24 5.460369175907097 1108 25 6.295264225706077 1109 1 3.7597922248125606 1109 2 -14.265255012511425 1109 3 -5.66282791647855 1109 4 2.211990634496196 1109 5 1.6596059779883525 1109 6 2.979463659456002 1109 7 4.160929064006201 1109 8 4.277938003116656 1109 9 3.7693857511188256 1109 10 2.899813070877819 1109 11 1.167207529148211 1109 12 1.8084163371014306 1109 13 2.91408583393795 1109 14 3.7337248126315536 1109 15 3.768641122370706 1109 16 3.6960090324156987 1109 17 4.396976052441861 1109 18 4.439422658330944 1109 19 2.804076570760955 1109 20 1.7453769449276493 1109 21 1.1494985286356254 1109 22 3.2238678333509583 1109 23 3.4242589541700923 1109 24 4.752328622287273 1109 25 5.0030628126205166 1110 1 3.130017397222005 1110 2 9.323307338560856 1110 3 6.4026258954407105 1110 4 7.764403736353302 1110 5 8.579720203185978 1110 6 4.156181134902628 1110 7 6.471941205161315 1110 8 7.420079032152046 1110 9 5.4734504535155395 1110 10 5.252759475809928 1110 11 2.999212017467233 1110 12 5.65223418195184 1110 13 10.243577620061988 1110 14 7.668025782158892 1110 15 6.896148466319669 1110 16 6.665699010604484 1110 17 6.929048622004505 1110 18 7.125733285308769 1110 19 5.849209392224196 1110 20 6.221954475595723 1110 21 3.9408043016729337 1110 22 6.068411878133286 1110 23 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5.302759213652152 2204 9 3.6776711165470495 2204 10 3.2443708940070692 2204 11 5.223792214304325 2204 12 4.7918281633704005 2204 13 3.642796805506066 2204 14 4.289463518370258 2204 15 4.4095795490234195 2204 16 4.375164797285752 2204 17 -1.1370201101257322 2204 18 2.6208111111479653 2204 19 3.5022625118040374 2204 20 3.8724134005355317 2204 21 5.5305381239609375 2204 22 4.8824758919590145 2204 23 3.699429022197304 2204 24 5.5504099060828445 2204 25 5.5780410090272845 2205 1 6.06271120426528 2205 2 10.749315539635816 2205 3 7.304289134840953 2205 4 7.973128525235314 2205 5 8.050213632092937 2205 6 7.798910538412084 2205 7 7.823637214751162 2205 8 7.501830300321835 2205 9 6.13950212077507 2205 10 5.2711174822129045 2205 11 7.863740784577171 2205 12 6.717823290066665 2205 13 10.215062073104374 2205 14 7.6589268770974215 2205 15 6.649471777741237 2205 16 5.831786615432549 2205 17 6.657181525224022 2205 18 6.496275808588997 2205 19 4.876026127679836 2205 20 5.341627797006723 2205 21 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0.40204289646195335 2207 10 -0.025881451253667365 2207 11 -2.1753077907359426 2207 12 -2.8753998988682916 2207 13 0.2820476989240923 2207 14 -5.952135524716645 2207 15 -1.8993617613721945 2207 16 -0.9447698267656648 2207 17 0.31244260972517424 2207 18 0.31952731831031045 2207 19 -0.15910101559595802 2207 20 -0.394943249973597 2207 21 -4.30493957654641 2207 22 -4.393811250812075 2207 23 -1.5538351652317814 2207 24 0.1685857228826677 2207 25 0.61235231803375 2208 1 4.59730238849199 2208 2 6.347959538342323 2208 3 6.20253779922124 2208 4 8.092121083112168 2208 5 3.7406219924119384 2208 6 6.166252643795097 2208 7 6.608521597678912 2208 8 6.34094220999614 2208 9 4.974791060978479 2208 10 4.346525341532182 2208 11 6.350616869127705 2208 12 6.031950115869062 2208 13 8.61547869991879 2208 14 2.7524418911481874 2208 15 4.483368735762017 2208 16 4.812525453386463 2208 17 5.84129322994389 2208 18 0.41740194210087367 2208 19 3.2372366796448944 2208 20 4.049039404714829 2208 21 6.799019510005159 2208 22 5.873873136140226 2208 23 1.540671086951935 2208 24 3.371733295812213 2208 25 4.708091624442201 2209 1 3.133601748622084 2209 2 5.021252923996514 2209 3 5.305426051775417 2209 4 0.7802446027501659 2209 5 5.069129787512161 2209 6 5.3635906435829845 2209 7 5.862516365593503 2209 8 4.662064987243639 2209 9 4.1376397193727925 2209 10 3.3379083089730557 2209 11 -0.7002527883121061 2209 12 2.8425433212284155 2209 13 2.0886890085411274 2209 14 3.7075343364410784 2209 15 4.0861014398310225 2209 16 3.523786810193478 2209 17 1.1572935127593205 2209 18 3.235454404362688 2209 19 3.6516956183800033 2209 20 4.019186468737171 2209 21 6.205847734984832 2209 22 4.818312277795403 2209 23 0.3726716525827882 2209 24 -3.7103697904111392 2209 25 -0.06757210801440294 2210 1 -2.059582191620681 2210 2 -14.265255012511425 2210 3 -7.7103535934159915 2210 4 -1.9450121666242524 2210 5 -0.24655648699764043 2210 6 -0.9288463312069304 2210 7 -0.7218440452374963 2210 8 -21.6776175524389 2210 9 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-5.417625496115516 2211 22 -6.881136202161553 2211 23 -8.657944435660418 2211 24 -22.979417409458758 2211 25 -22.816778457220753 2212 1 -0.9364219250690731 2212 2 -14.265255012511425 2212 3 -7.919217872624008 2212 4 -20.27610460359904 2212 5 -20.76420354582117 2212 6 -21.507837927845586 2212 7 -10.034163496245283 2212 8 -9.740372238665469 2212 9 -5.094692333252656 2212 10 -5.274706663666368 2212 11 -6.015114821144799 2212 12 -2.8875086832734667 2212 13 -6.720473874215889 2212 14 -3.840519375671313 2212 15 -3.907359188103932 2212 16 -3.9880092855879496 2212 17 -4.017841349661605 2212 18 -3.22793869533848 2212 19 -3.849441494982049 2212 20 -3.8875760569740088 2212 21 -1.9235406299781062 2212 22 -3.240458630731009 2212 23 -3.4441017397407236 2212 24 -2.263850987290869 2212 25 -2.6513342844416172 2213 1 -19.353699838679503 2213 2 -14.265255012511425 2213 3 -8.416964733447225 2213 4 -5.161945311563642 2213 5 -20.76420354582117 2213 6 -10.679519343774789 2213 7 -21.403356650279513 2213 8 -10.645759145359253 2213 9 -8.176393150412288 2213 10 -6.691569145725637 2213 11 -6.285735299900814 2213 12 -4.818427320715678 2213 13 -19.91607289622078 2213 14 -12.445886087775806 2213 15 -6.679034112915147 2213 16 -5.843673507266839 2213 17 -4.385928849932068 2213 18 -4.46105493683692 2213 19 -5.4606235275674635 2213 20 -5.354229741404701 2213 21 -3.7150891943591766 2213 22 -4.516376582999655 2213 23 -4.345631479879636 2213 24 -3.267028028927786 2213 25 -4.234477572265 2214 1 -19.353699838679503 2214 2 -14.265255012511425 2214 3 -12.002375503394559 2214 4 -20.27610460359904 2214 5 -20.76420354582117 2214 6 -21.507837927845586 2214 7 -16.577445719105423 2214 8 -14.720127673896389 2214 9 -13.15819602383137 2214 10 -13.157079159698624 2214 11 -14.111868365717157 2214 12 -11.64124628098224 2214 13 -19.91607289622078 2214 14 -16.716175579374102 2214 15 -24.763104909375325 2214 16 -18.300556098359955 2214 17 -24.48238902692322 2214 18 -17.843268042780814 2214 19 -14.420979659558434 2214 20 -12.9043981337948 2214 21 -10.490097255697 2214 22 -11.576315316832194 2214 23 -11.487260228401723 2214 24 -10.227393117960782 2214 25 -10.011110441026421 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 113691.0 3 2.0 4 505.0 5 1950.0 6 2740.0 7 2762.0 8 2377.0 9 2331.0 10 2086.0 11 2068.0 12 2049.0 13 2059.0 14 3338.0 15 7474.0 16 11167.0 17 11578.0 18 11855.0 19 14030.0 20 16620.0 21 19193.0 22 19874.0 23 21760.0 24 22787.0 25 23489.0 26 19726.0 27 14993.0 28 15950.0 29 18583.0 30 18090.0 31 20936.0 32 28101.0 33 35373.0 34 43587.0 35 51031.0 36 51573.0 37 54230.0 38 42914.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45638319871164 18.434607696308504 11.762217780089358 26.346791324890496 2 14.318809949601691 20.11183461824778 41.43784921578862 24.13150621636191 3 19.088027528629947 24.055218921314193 28.974200569856496 27.882552980199364 4 11.253207007192522 17.046464570056166 38.66720878483582 33.03311963791549 5 12.316972880319877 38.58953716628174 36.30624726266735 12.787242690731038 6 31.35938444171983 37.728135866697066 18.529407544287892 12.383072147295215 7 27.455687159240462 30.87828539199098 23.18390674938898 18.482120699379585 8 24.14926284941056 38.36886894493984 20.608047786777476 16.873820418872118 9 26.18409513740339 15.139097200025134 19.903070437849518 38.773737224721955 10 13.574644351348622 29.79255500980551 35.839723868006914 20.793076770838955 11 33.54298995265042 21.99084767294145 25.380528929437578 19.08563344497055 12 23.198200149602457 24.48094012724873 32.78907497740079 19.531784745748027 13 28.442373156103095 21.526503485167776 25.703355487112983 24.327767871616146 14 19.682015188203152 22.556241952317524 28.08306802258133 29.67867483689799 15 22.021619070627168 32.42003809181363 23.411436533307864 22.14690630425134 16 21.11544550533308 28.278480692591557 28.992929958405732 21.613143843669622 17 20.874350085438405 28.734261993319375 29.26444344286579 21.12694447837643 18 20.715051365926485 27.731368253115328 31.38293305118312 20.170647329775065 19 22.894244397200815 26.841809504827708 27.396829878642926 22.86711621932855 20 22.817235230766915 28.05165588107707 28.612579523481752 20.51852936467426 21 21.660190825876377 26.707070591890897 26.853026445946448 24.779712136286278 22 21.97929084305952 28.944720135288737 28.075046436195283 21.00094258545646 23 21.478508650906615 28.396638536080275 28.489800044258544 21.63505276875456 24 22.423263715879145 27.255713573902764 29.0239648931125 21.297057817105603 25 22.105500420061883 27.837603393280375 28.628685786881775 21.428210399775967 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 645.0 1 645.0 2 1425.5 3 2206.0 4 2206.0 5 2206.0 6 3205.5 7 4205.0 8 4205.0 9 4205.0 10 4878.5 11 5552.0 12 5552.0 13 5552.0 14 6850.0 15 8148.0 16 8148.0 17 8148.0 18 11921.0 19 15694.0 20 15694.0 21 15694.0 22 22116.5 23 28539.0 24 28539.0 25 28539.0 26 38033.0 27 47527.0 28 47527.0 29 47527.0 30 58371.5 31 69216.0 32 69216.0 33 69216.0 34 76286.5 35 83357.0 36 83357.0 37 83357.0 38 86990.0 39 90623.0 40 90623.0 41 90623.0 42 91687.0 43 92751.0 44 92751.0 45 92751.0 46 90600.0 47 88449.0 48 88449.0 49 88449.0 50 80825.5 51 73202.0 52 73202.0 53 73202.0 54 62781.0 55 52360.0 56 52360.0 57 52360.0 58 43371.5 59 34383.0 60 34383.0 61 34383.0 62 27034.0 63 19685.0 64 19685.0 65 19685.0 66 14788.0 67 9891.0 68 9891.0 69 9891.0 70 7059.0 71 4227.0 72 4227.0 73 4227.0 74 2862.5 75 1498.0 76 1498.0 77 1498.0 78 1005.0 79 512.0 80 512.0 81 512.0 82 328.5 83 145.0 84 145.0 85 145.0 86 95.5 87 46.0 88 46.0 89 46.0 90 27.0 91 8.0 92 8.0 93 8.0 94 5.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.899933412655962 2 36.213144996670636 3 5.466984684910871 4 13.416258227903372 5 13.38978702965866 6 14.41847962536432 7 9.278154984772238 8 7.531328799572094 9 0.10834088353764368 10 1.4080221375629032 11 5.478719339802858 12 5.508465325459289 13 15.824318571319413 14 1.554295975286271 15 2.8528856335076247 16 2.8647567378750995 17 2.8194555120130116 18 1.4733814363217588 19 1.4157997576657315 20 0.28831774170660085 21 4.269504088026286 22 1.5626193932910524 23 0.11052407514545513 24 9.082622886397624 25 0.11420821098363698 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 732872.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.67515443123143 #Duplication Level Percentage of deduplicated Percentage of total 1 96.89936197259345 89.80163335097893 2 2.3263286205394396 4.311857283325723 3 0.34434250077746104 0.9573598331036289 4 0.14257011076045223 0.5285082812801071 5 0.07195946819770307 0.33344274140057095 6 0.04507491533733337 0.2506394843917232 7 0.03218157549033403 0.20877027348849267 8 0.02028426387335403 0.15038778295895466 9 0.01737758227044464 0.14494231084993978 >10 0.08751942364165072 1.5034290423706176 >50 0.0060465346970891365 0.3950504792116947 >100 0.006348420365772045 1.017129238547469 >500 6.046114556156355E-4 0.3968498980921504 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 916 0.12498771954720606 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.3644947548821623E-4 0.0 12 0.0 0.0 0.0 1.3644947548821623E-4 0.0 13 0.0 0.0 0.0 1.3644947548821623E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTAGGC 25 0.00265354 21.89142 3 TCCTATA 50 9.355952E-5 19.144648 2 GTCCTAT 75 4.3198634E-7 17.789677 1 TGTAGGA 580 0.0 16.504007 2 GGACTGC 40 0.0028526178 15.629198 6 TAGGACC 280 0.0 15.308804 4 CAATACT 70 2.076773E-6 15.308804 4 ATAATAC 75 3.4121404E-6 14.594282 3 GTCTATG 55 0.004578963 14.555189 1 GTCTTAA 55 0.004578963 14.555189 1 GTGCTAG 65 8.347221E-4 14.368584 1 GTAGGAC 620 0.0 14.123498 3 GGACGTG 510 0.0 14.096923 6 AGGACGT 540 0.0 14.090845 5 GACGTGG 225 0.0 14.032812 7 CTGTAGG 580 0.0 14.032373 1 TAGGACG 590 0.0 13.985498 4 CCTATAG 75 5.512668E-5 13.134854 3 ATAAAGC 50 0.009042042 13.134852 3 CTAGGAC 100 3.4633013E-7 13.134852 3 >>END_MODULE