FastQCFastQC Report
Thu 26 May 2016
SRR1926438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926438_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732872
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA19130.2610278466089576No Hit
GTCCTACAGTGGACATTTCTAAATT17350.23673983997205517No Hit
GTCCTAAAGTGTGTATTTCTCATTT14510.19798818893340173No Hit
CTTTAGGACGTGAAATATGGCGAGG13540.18475258981104475No Hit
GTCCTACAGTGTGCATTTCTCATTT12370.16878800117892345No Hit
CTGTAGGACCTGGAATATGGCGAGA9770.13331113755198726No Hit
CTGAAGGACCTGGAATATGGCGAGA7670.10465674769946184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGCG307.708781E-419.0031497
TAGGACC3750.013.4814934
TAAGACC801.9059989E-613.1158394
GACGTGG5450.013.0755617
GGACCTG5800.012.66356856
ACGTGGA5650.012.4462448
TGTAGGA11700.012.3158262
CGTGGAA5600.012.2179829
AGGACCT6350.012.1675935
TAGGGTG550.002973588912.1402815
TAGGACA3150.012.1127524
GTAGGAC11650.012.03693
TTTACAC802.7426384E-511.924313
GTCCTAA4250.011.8913491
CTAGGAC1052.570032E-711.8107453
GGACGTG9200.011.6124436
CTGTAGG12000.011.6015441
GTCCTAC9750.011.5404191
AGGACGT9600.011.526045
CCTACAC1251.7162165E-811.4473373
Produced by FastQC (version 0.11.5)