FastQCFastQC Report
Thu 26 May 2016
SRR1926437_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926437_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730437
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11270.15429119828267188No Hit
GTCCTAAAGTGTGTATTTCTCATTT8240.11280918135308042No Hit
CTGTAGGACGTGGAATATGGCAAGA7960.1089758596566165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG454.877372E-520.8371241
TTATACC451.2345767E-519.084044
TAGGACC2300.016.3355244
TGTAGGA7600.015.5434412
GTGTAAG952.860961E-715.510341
GTCCTAG1055.3219992E-815.3089071
CTGTAGG7150.014.9877411
CAAGTCG507.2882493E-414.6294766
ATACCTA450.006121617713.9328346
GTAGTGC450.006121617713.9328346
GTAGGAC8050.013.9053813
TCCTAGG802.0557854E-413.7360642
GTCTATA904.5805908E-513.3952941
CTCTTAT600.00793856713.3952941
CTATACT1055.5028067E-813.290674
GTCCTAC7900.013.2257331
CTTAGAG755.3789023E-513.1692143
TCTATAC1107.575363E-812.9696813
TCCTACA8000.012.534162
ATCTAAT1009.712465E-512.3624582
Produced by FastQC (version 0.11.5)