##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926437_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 730437 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.008685211729418 27.0 10.0 31.0 2.0 31.0 2 16.845539040327914 22.0 2.0 31.0 2.0 31.0 3 20.64925790999087 25.0 15.0 31.0 2.0 31.0 4 22.93337823795892 28.0 10.0 35.0 2.0 37.0 5 23.525653820931854 30.0 10.0 35.0 2.0 37.0 6 24.27057227385798 31.0 10.0 35.0 2.0 37.0 7 24.120410110659783 31.0 10.0 35.0 2.0 37.0 8 24.406432039997974 32.0 17.0 35.0 2.0 37.0 9 27.300003970226044 32.0 18.0 38.0 2.0 39.0 10 28.054125133310606 33.0 26.0 39.0 2.0 39.0 11 25.029274256369817 31.0 10.0 37.0 2.0 39.0 12 26.2646141967069 31.0 17.0 37.0 2.0 39.0 13 22.727550767554217 29.0 2.0 37.0 2.0 39.0 14 26.285593418734265 31.0 17.0 38.0 2.0 40.0 15 27.448505483703592 32.0 22.0 39.0 2.0 40.0 16 27.816294902914283 32.0 24.0 39.0 2.0 41.0 17 27.14564431977022 31.0 18.0 39.0 2.0 40.0 18 27.26583127634553 31.0 20.0 39.0 2.0 40.0 19 27.673427003287074 31.0 24.0 39.0 2.0 40.0 20 27.941882735951218 32.0 26.0 39.0 2.0 41.0 21 25.757509545655545 31.0 10.0 38.0 2.0 40.0 22 26.717545797926448 31.0 18.0 39.0 2.0 40.0 23 26.736667227974486 31.0 18.0 39.0 2.0 40.0 24 25.692059684818815 31.0 2.0 39.0 2.0 40.0 25 25.511945588736605 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.727061540710281 1101 2 10.567610840740432 1101 3 7.049705084962444 1101 4 8.506704168683381 1101 5 8.286122509094 1101 6 7.8309559623738565 1101 7 7.432668354124743 1101 8 6.109179021513764 1101 9 5.906888367218226 1101 10 5.2124518821771595 1101 11 8.347190289714263 1101 12 5.925390741492908 1101 13 10.600354204968557 1101 14 7.885536706968313 1101 15 6.976790713866219 1101 16 6.547553827410447 1101 17 2.24392397730794 1101 18 4.811973822080287 1101 19 5.408370690092376 1101 20 3.5348445762494585 1101 21 1.0696724835892866 1101 22 4.500768528221801 1101 23 5.730034163929762 1101 24 7.3233241404263545 1101 25 7.525676209664216 1102 1 6.0675253031383285 1102 2 11.301827219599666 1102 3 7.286280830645332 1102 4 8.915706654293007 1102 5 8.433903121338897 1102 6 7.19113845844921 1102 7 7.188891535705068 1102 8 7.316695374208688 1102 9 6.3817621244134095 1102 10 5.915433959623524 1102 11 8.612320457165858 1102 12 5.6811900612208 1102 13 10.269637956930882 1102 14 7.877668444278623 1102 15 5.80608493155329 1102 16 6.218089541696159 1102 17 6.98688316098141 1102 18 7.08939467503685 1102 19 5.839140574969406 1102 20 6.290702765679079 1102 21 5.467538129847792 1102 22 6.533866382748222 1102 23 6.979754859900456 1102 24 8.325515401546188 1102 25 8.100183666493106 1103 1 5.215682299162282 1103 2 9.77880080612401 1103 3 6.638044973650956 1103 4 8.155092924565064 1103 5 7.549991749191008 1103 6 7.156233075115601 1103 7 7.038731669009806 1103 8 6.616552500567721 1103 9 5.544332530101958 1103 10 4.956737970314304 1103 11 5.974923199151899 1103 12 4.5253132691134965 1103 13 8.849435030165996 1103 14 7.249635514049128 1103 15 6.007922821114935 1103 16 5.853359203319904 1103 17 3.5031196119077777 1103 18 2.6208819797404637 1103 19 4.594748117905738 1103 20 4.899407828949329 1103 21 7.317861987766062 1103 22 3.0419092118442954 1103 23 5.24252885227833 1103 24 6.529135087092197 1103 25 2.5589083625154068 1104 1 5.910661545777675 1104 2 10.747193033909582 1104 3 7.312716258571442 1104 4 7.933152810621152 1104 5 7.929823101979377 1104 6 7.524002861127276 1104 7 7.359516686951338 1104 8 7.363396995568696 1104 9 6.311141813047758 1104 10 5.2714104055378606 1104 11 8.366047971887163 1104 12 5.466118672981711 1104 13 9.68415437219264 1104 14 7.279310777101077 1104 15 6.51378840820087 1104 16 5.877716870888705 1104 17 6.5127974950945 1104 18 3.8924244403026442 1104 19 4.900765161562937 1104 20 3.9554410088024134 1104 21 3.1983697826670223 1104 22 5.3687780549269775 1104 23 5.7672421552138395 1104 24 6.678552426632905 1104 25 5.820190119667259 1105 1 4.2889416810102325 1105 2 10.36922549966641 1105 3 6.974974194783439 1105 4 6.59558600225861 1105 5 2.79929074808771 1105 6 5.540302237168245 1105 7 6.6022075413526125 1105 8 3.728539810096251 1105 9 4.640061444141303 1105 10 4.24237145160145 1105 11 7.582600908969219 1105 12 4.686003949040078 1105 13 5.914411413483315 1105 14 4.406808477650461 1105 15 5.015509267325335 1105 16 5.2124516261525855 1105 17 5.762791901836241 1105 18 3.2534499952781566 1105 19 2.8373891951722037 1105 20 2.432703792504899 1105 21 3.0800891502559544 1105 22 4.851155562571098 1105 23 2.2120279955698194 1105 24 5.2311918230581895 1105 25 6.015985232402485 1106 1 6.444885174443883 1106 2 11.085516460365525 1106 3 7.67751762211617 1106 4 9.153492352807344 1106 5 8.623013931041449 1106 6 7.687642890206455 1106 7 7.563466812492756 1106 8 7.079491954973914 1106 9 6.119839908850214 1106 10 5.42396185919139 1106 11 8.11182018905243 1106 12 5.390067454527429 1106 13 4.6453733762918255 1106 14 5.772149723652813 1106 15 6.010198106962559 1106 16 5.7493988815062735 1106 17 6.210500484263118 1106 18 6.28517236169111 1106 19 6.201735282094916 1106 20 5.666334093944542 1106 21 5.9701587020333875 1106 22 6.200853734102985 1106 23 6.727085396442742 1106 24 7.756578707857894 1106 25 6.771778783674442 1107 1 4.177983938205259 1107 2 -14.363478902849312 1107 3 -5.864757132927194 1107 4 1.8650987982473666 1107 5 4.399169744117042 1107 6 4.842975193143086 1107 7 4.761755261840683 1107 8 5.433188681208907 1107 9 3.6127956991336205 1107 10 3.0130508128164166 1107 11 5.824337134804654 1107 12 2.9738738444114965 1107 13 4.499802538892698 1107 14 3.9336394778533403 1107 15 3.6175837246189033 1107 16 3.4618683435394786 1107 17 4.040966988060628 1107 18 -1.36428976648169 1107 19 1.7016509984936548 1107 20 2.0733799655913003 1107 21 -1.6766312798515735 1107 22 1.6519265100249427 1107 23 -1.067802743786178 1107 24 2.2193051755202973 1107 25 2.9688516498154627 1108 1 5.09062235634476 1108 2 10.438531924683556 1108 3 0.28067324190217846 1108 4 5.695302910428875 1108 5 7.229785502133442 1108 6 7.135705278216559 1108 7 7.202371340620076 1108 8 6.951832138891358 1108 9 5.373433447234884 1108 10 4.283977924773392 1108 11 6.890271749051518 1108 12 2.2308335102477415 1108 13 3.477308143334909 1108 14 4.8800132522714215 1108 15 4.768046514330255 1108 16 4.557632133674947 1108 17 -0.47717037690706476 1108 18 3.349422138458415 1108 19 4.516034367289116 1108 20 4.8520481576641785 1108 21 4.91699943641218 1108 22 1.4494716039550362 1108 23 4.213545601255003 1108 24 5.222117197717093 1108 25 5.716714120902246 1109 1 3.5615919467449224 1109 2 -14.363478902849312 1109 3 -5.979131368132485 1109 4 1.8168158437314972 1109 5 1.3143458747114316 1109 6 2.6391818113836045 1109 7 3.748854639590693 1109 8 3.9628431892669163 1109 9 3.434855633003288 1109 10 2.5555538719952544 1109 11 0.7079321255620279 1109 12 1.3538108383452112 1109 13 3.2773676928930655 1109 14 3.446657260143951 1109 15 3.4850887364650873 1109 16 3.247790913770416 1109 17 3.777317843328923 1109 18 3.910775973318877 1109 19 2.3303708297831953 1109 20 1.517065039402123 1109 21 0.667155332661121 1109 22 2.8961401057967677 1109 23 3.3547023982378263 1109 24 4.435973143965192 1109 25 4.666110104865062 1110 1 2.8972840564799185 1110 2 9.289875729738547 1110 3 6.544106350629683 1110 4 7.610811955199313 1110 5 8.111302221554062 1110 6 4.003518324133502 1110 7 6.465502998181435 1110 8 7.300982392530393 1110 9 5.091605640094443 1110 10 4.878460627159551 1110 11 2.555582503198327 1110 12 5.462464756402387 1110 13 9.952920867539216 1110 14 7.528249392312784 1110 15 6.616999712801256 1110 16 6.314826191627702 1110 17 6.850553370279183 1110 18 6.972990554283122 1110 19 5.94800215154892 1110 20 6.240126381770878 1110 21 4.1212233355594705 1110 22 6.003123734251126 1110 23 6.388992650863489 1110 24 7.435119626622804 1110 25 7.965595415857383 1111 1 4.749785899684639 1111 2 9.246521097150687 1111 3 7.0126858541932116 1111 4 7.817825963555176 1111 5 7.756603278324768 1111 6 7.209129039296933 1111 7 7.039142713099103 1111 8 6.933634149718891 1111 9 5.354164008243867 1111 10 4.483958292433574 1111 11 6.610106956380925 1111 12 5.64340356200573 1111 13 7.726027281891636 1111 14 5.293068758250364 1111 15 4.864515072840575 1111 16 4.880726904333521 1111 17 6.115077823461785 1111 18 5.870980232336699 1111 19 4.760710433682121 1111 20 4.477280473685354 1111 21 7.068166073332879 1111 22 6.371024938478207 1111 23 6.370963651109246 1111 24 6.429926704528921 1111 25 6.7726954404334485 1112 1 -2.2183103882257456 1112 2 7.668279424029077 1112 3 5.962289619908521 1112 4 -0.5671248795598807 1112 5 3.9240727414720027 1112 6 3.188831118782126 1112 7 5.873544786625885 1112 8 5.750915327100767 1112 9 4.252829324337533 1112 10 3.067650719294086 1112 11 -1.1038160609580459 1112 12 3.5096116296932713 1112 13 8.070683183697035 1112 14 5.45100536076481 1112 15 3.5026024231355173 1112 16 3.575221333219897 1112 17 4.572642025332726 1112 18 4.442589003116652 1112 19 3.9160390400099523 1112 20 3.837817326453038 1112 21 -2.247660656095711 1112 22 1.7224561486313412 1112 23 3.395543285262857 1112 24 5.43421139737287 1112 25 5.352262264004921 1113 1 -19.493291023392285 1113 2 -14.363478902849312 1113 3 -7.648890814171535 1113 4 -6.322293751167187 1113 5 -1.1039851068000388 1113 6 -0.49063407838009354 1113 7 -21.540088056131665 1113 8 -10.094776446307598 1113 9 -5.431406547028676 1113 10 -4.4816122628389685 1113 11 -5.383828346726563 1113 12 -3.3396478898536692 1113 13 -2.0181347150518185 1113 14 -2.6109556941184593 1113 15 -3.094746261851224 1113 16 -3.3144080931193542 1113 17 -2.9721356253410676 1113 18 -4.010815488498761 1113 19 -3.3459222713611787 1113 20 -4.180493341903031 1113 21 -1.6550101874923904 1113 22 -2.7496347660556673 1113 23 -3.2643037966747563 1113 24 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4.472188676289154 2204 7 5.874839712728686 2204 8 5.8338428174556505 2204 9 4.189686912306094 2204 10 3.701680233413942 2204 11 5.174274137514399 2204 12 5.375063026137106 2204 13 3.1744258496160462 2204 14 4.69626051031852 2204 15 5.078499515364342 2204 16 4.671701098678504 2204 17 -0.20651743521212396 2204 18 3.467032831423005 2204 19 4.197448302554822 2204 20 4.515577794342228 2204 21 5.9450570475272215 2204 22 5.556567598065815 2204 23 4.351215534057761 2204 24 5.987060918629428 2204 25 6.169424666263797 2205 1 5.9780365492707155 2205 2 10.84390522912241 2205 3 7.195573037708833 2205 4 7.8532426050440876 2205 5 8.024167481599893 2205 6 7.488301194714179 2205 7 7.654727340569043 2205 8 7.5246239376214845 2205 9 6.236749058700088 2205 10 5.554375236055545 2205 11 7.951015168357479 2205 12 6.940201555842215 2205 13 10.143036164603583 2205 14 7.5057069841250375 2205 15 6.449206594986929 2205 16 5.932397700152002 2205 17 6.718491927032996 2205 18 6.467870074623242 2205 19 5.346403829053443 2205 20 5.657452689529215 2205 21 -0.9390585323834806 2205 22 3.7795819509865183 2205 23 5.173160762717199 2205 24 6.915861202081647 2205 25 7.44092977483432 2206 1 4.2495881144060235 2206 2 9.123049195688026 2206 3 6.2137754497400834 2206 4 1.839554512142847 2206 5 5.772530322796847 2206 6 6.066608757423303 2206 7 6.393707170966103 2206 8 6.232933610842835 2206 9 4.818467910654004 2206 10 3.788854958508068 2206 11 3.4861932949845844 2206 12 4.689873035565274 2206 13 8.396367780500032 2206 14 -0.1634451281711442 2206 15 2.4378424844589794 2206 16 3.602187199234926 2206 17 4.500563700171682 2206 18 -0.7435996219891159 2206 19 1.15327986578966 2206 20 2.653523145563131 2206 21 3.5249505821266247 2206 22 -1.7685954795948646 2206 23 -2.687052574389657 2206 24 1.2910618972807413 2206 25 2.8795486411203655 2207 1 1.7985408560684597 2207 2 -14.363478902849312 2207 3 -6.450914463015351 2207 4 -3.9777410467541046 2207 5 1.4369204868893988 2207 6 2.166131418361168 2207 7 3.3068588114337594 2207 8 3.5004937849290414 2207 9 0.9347349836602028 2207 10 0.5040772456453091 2207 11 -1.2087590230005176 2207 12 -2.1351927900957755 2207 13 0.5603809694412014 2207 14 -5.742974064905862 2207 15 -1.5674119691895605 2207 16 -0.24068467377908576 2207 17 0.7543323833349014 2207 18 0.7566027541447866 2207 19 0.3348262148082135 2207 20 0.004346294462514777 2207 21 -3.6192566070794925 2207 22 -4.348491318460727 2207 23 -0.9749205677646628 2207 24 0.8628926046082235 2207 25 1.2574614991170314 2208 1 4.668973127551112 2208 2 6.36784185186767 2208 3 6.222961616167087 2208 4 8.116214933076222 2208 5 3.4613347732449427 2208 6 6.137692174275163 2208 7 6.730855340094749 2208 8 6.644011508209459 2208 9 4.993960815210485 2208 10 4.519792835249245 2208 11 6.497726695132741 2208 12 6.183708351555801 2208 13 9.014851665055641 2208 14 3.207248729222787 2208 15 4.479754550344204 2208 16 5.0577660915613905 2208 17 6.097980581081526 2208 18 0.6509657185485977 2208 19 3.1579237863671707 2208 20 4.298804421435719 2208 21 7.042447640821994 2208 22 6.040589524835248 2208 23 1.8517764232427751 2208 24 3.722481131234293 2208 25 4.986236804729529 2209 1 3.457890078969921 2209 2 5.205812435733364 2209 3 5.383128010462141 2209 4 0.3877169387217414 2209 5 5.172884320117621 2209 6 5.849274405741511 2209 7 6.226841077726601 2209 8 4.901901866254324 2209 9 4.413158557002195 2209 10 3.396417408121035 2209 11 -0.23943271654457376 2209 12 3.2000298491044568 2209 13 2.8237983297414253 2209 14 3.815630841836068 2209 15 4.190160136438394 2209 16 3.8258510715049354 2209 17 1.6248294891189623 2209 18 3.2149354110162847 2209 19 4.001994503942569 2209 20 3.922301672958522 2209 21 6.183829307736268 2209 22 4.939522818550895 2209 23 0.5230169521389278 2209 24 -2.879964270637643 2209 25 0.2790491654485905 2210 1 -1.9024196540976774 2210 2 -14.363478902849312 2210 3 -7.872408304759867 2210 4 -2.098844958883383 2210 5 -0.3450756236860819 2210 6 -1.043746798558157 2210 7 -1.0280548611109168 2210 8 -21.856365850281108 2210 9 -13.545069952815822 2210 10 -7.117059900991261 2210 11 -10.507374690602795 2210 12 -4.756957114662001 2210 13 -5.712670451909833 2210 14 -4.053069128758892 2210 15 -5.984058179633092 2210 16 -5.301175425701587 2210 17 -4.670910047588325 2210 18 -4.853689413370294 2210 19 -10.54714784911392 2210 20 -7.043693034134627 2210 21 -5.11897723978554 2210 22 -9.701056133976305 2210 23 -7.6667893013325 2210 24 -10.996306937347875 2210 25 -8.330857065152252 2211 1 -0.07211074853375621 2211 2 4.883893772979306 2211 3 0.42705063250301833 2211 4 1.2779708550420246 2211 5 1.2759416279298925 2211 6 -0.17278875188520715 2211 7 -21.540088056131665 2211 8 -13.661539657880137 2211 9 -9.146855828830944 2211 10 -7.111168254422967 2211 11 -9.812465644825465 2211 12 -4.752778889334362 2211 13 -7.259699973876041 2211 14 -4.774616709071321 2211 15 -5.118188739111417 2211 16 -11.609858258280742 2211 17 -7.527599870711462 2211 18 -5.491486654050966 2211 19 -8.424653660590621 2211 20 -6.485394111228519 2211 21 -3.9586133558071026 2211 22 -5.616134442157321 2211 23 -7.579621511505017 2211 24 -23.184688680086463 2211 25 -23.029612251874244 2212 1 -1.0425180372246672 2212 2 -14.363478902849312 2212 3 -8.056238224169434 2212 4 -20.40755865182944 2212 5 -20.932627490906 2212 6 -21.657024806856914 2212 7 -10.284595785993341 2212 8 -9.89623566313709 2212 9 -5.154961609832117 2212 10 -5.828234860223372 2212 11 -6.114411413776175 2212 12 -3.173656586958405 2212 13 -6.37744395801573 2212 14 -4.098159257021592 2212 15 -4.196173416863374 2212 16 -4.198555814574906 2212 17 -4.366633386402395 2212 18 -3.3919045395228515 2212 19 -3.8948744696163686 2212 20 -4.19832132890587 2212 21 -1.9670019807448575 2212 22 -3.29297143130335 2212 23 -3.336748685374449 2212 24 -2.191198037287439 2212 25 -2.7846976871875064 2213 1 -19.493291023392285 2213 2 -14.363478902849312 2213 3 -9.025224358461028 2213 4 -6.006534761044456 2213 5 -20.932627490906 2213 6 -11.264533339280122 2213 7 -21.540088056131665 2213 8 -11.327355611373259 2213 9 -8.772339010921268 2213 10 -7.652749504888558 2213 11 -6.8142721457456155 2213 12 -5.466176380236327 2213 13 -20.046280410416056 2213 14 -13.119454213658274 2213 15 -7.667656100695787 2213 16 -6.739028937094673 2213 17 -5.403260323033358 2213 18 -5.510778759524683 2213 19 -6.358483580494827 2213 20 -6.622359851859407 2213 21 -4.706173648378858 2213 22 -5.62212024398438 2213 23 -5.4434338285442045 2213 24 -4.3903200794038675 2213 25 -5.190021808188238 2214 1 -19.493291023392285 2214 2 -14.363478902849312 2214 3 -12.500467917350472 2214 4 -20.40755865182944 2214 5 -20.932627490906 2214 6 -21.657024806856914 2214 7 -16.938730151185013 2214 8 -15.364610273171506 2214 9 -14.092803088674735 2214 10 -14.080205700702292 2214 11 -14.973088596853424 2214 12 -12.428493028309871 2214 13 -20.046280410416056 2214 14 -17.248254079920194 2214 15 -24.887792619467117 2214 16 -18.897554077536206 2214 17 -24.63107602269206 2214 18 -18.42800432335979 2214 19 -15.254877121342872 2214 20 -13.95176600844551 2214 21 -11.509265844894385 2214 22 -12.525873517091439 2214 23 -12.482254285173674 2214 24 -11.230275489009838 2214 25 -11.10817675235921 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 113344.0 3 0.0 4 497.0 5 1976.0 6 2724.0 7 2762.0 8 2341.0 9 2271.0 10 2029.0 11 1896.0 12 1910.0 13 1997.0 14 3332.0 15 7542.0 16 11339.0 17 11807.0 18 12262.0 19 14124.0 20 16432.0 21 19214.0 22 20082.0 23 21434.0 24 22490.0 25 23448.0 26 19581.0 27 14611.0 28 15354.0 29 18291.0 30 17625.0 31 20210.0 32 26871.0 33 34541.0 34 43014.0 35 51145.0 36 51407.0 37 55123.0 38 45411.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32638759140037 18.54766436232706 11.899860979602952 26.226087066669624 2 13.955959594207174 20.157355320284005 42.02098360086918 23.86570148463964 3 19.025337842750083 24.265789986419406 28.952985549897782 27.755886620932728 4 11.115752225882174 17.12047239842576 38.969852518431225 32.79392285726084 5 11.91906075853487 38.68037719876134 36.71115906190426 12.689402980799528 6 31.088468584299644 37.93987377972839 18.697324304249825 12.274333331722149 7 27.28753376140629 30.896715049194114 23.3740839430298 18.441667246369796 8 23.86955928098212 38.744865006055136 20.617000688623467 16.768575024339277 9 26.282598654318228 15.107738149900014 20.181114676264713 38.42854851951705 10 13.647139633282396 29.76608439204606 36.038270779250574 20.548505195420965 11 33.230618889666076 21.929218647979326 25.89378289201023 18.94637957034437 12 23.44257626567013 24.375955318691407 33.0303435369603 19.151124878678157 13 28.553371891721753 21.74171781554926 25.66985363550519 24.035056657223798 14 19.511909850777027 22.914366314065834 28.20908760531728 29.364636229839864 15 21.767372241128545 32.95253964443566 23.34834856037392 21.931739554061878 16 20.746840469686457 28.14458587294611 29.72339712925422 21.385176528113213 17 20.671790854101822 28.919191705309817 29.483840458494786 20.92517698209358 18 20.4517025808992 27.564932941701926 32.0711058670967 19.912258610302167 19 22.83956196766904 26.933286980705716 27.48492960194681 22.74222144967843 20 22.750617256825528 28.134277733987712 28.884049094918858 20.231055914267902 21 21.427854576526823 26.80324131820489 27.000805741698137 24.76809836357015 22 21.9270268990471 29.429902871515758 28.011693300167963 20.631376929269184 23 21.06973582133331 28.712057040880946 28.805261094709024 21.41294604307672 24 22.39876165666176 27.329029335145545 29.300411522633745 20.971797485558955 25 21.731526016615653 27.995772754001425 28.988709524873453 21.28399170450947 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 728.0 1 728.0 2 1596.5 3 2465.0 4 2465.0 5 2465.0 6 3562.0 7 4659.0 8 4659.0 9 4659.0 10 5289.5 11 5920.0 12 5920.0 13 5920.0 14 7220.5 15 8521.0 16 8521.0 17 8521.0 18 12354.0 19 16187.0 20 16187.0 21 16187.0 22 23024.0 23 29861.0 24 29861.0 25 29861.0 26 39805.0 27 49749.0 28 49749.0 29 49749.0 30 60588.0 31 71427.0 32 71427.0 33 71427.0 34 78293.0 35 85159.0 36 85159.0 37 85159.0 38 88607.5 39 92056.0 40 92056.0 41 92056.0 42 91692.5 43 91329.0 44 91329.0 45 91329.0 46 89045.0 47 86761.0 48 86761.0 49 86761.0 50 78857.0 51 70953.0 52 70953.0 53 70953.0 54 60155.5 55 49358.0 56 49358.0 57 49358.0 58 40821.5 59 32285.0 60 32285.0 61 32285.0 62 25250.5 63 18216.0 64 18216.0 65 18216.0 66 13616.0 67 9016.0 68 9016.0 69 9016.0 70 6397.5 71 3779.0 72 3779.0 73 3779.0 74 2573.0 75 1367.0 76 1367.0 77 1367.0 78 906.0 79 445.0 80 445.0 81 445.0 82 296.5 83 148.0 84 148.0 85 148.0 86 93.0 87 38.0 88 38.0 89 38.0 90 23.5 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.536417240638137 2 36.8703940243991 3 5.844583447990724 4 13.799821202923729 5 13.966296888027305 6 15.028811519679316 7 9.47090577284557 8 7.752756226751931 9 0.11554726827912606 10 1.539626278515464 11 5.855398891348604 12 5.7753098487617684 13 16.49053922514878 14 1.677078242202955 15 3.0484490791129146 16 3.1236095652328677 17 3.0162765577318784 18 1.6064355995109778 19 1.5486619653714147 20 0.30324312705955475 21 4.510039880236079 22 1.700215076728041 23 0.11705321608845116 24 9.345364487286378 25 0.12102344213121734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 730437.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.82602843568671 #Duplication Level Percentage of deduplicated Percentage of total 1 96.96262992090465 89.03693212315954 2 2.1818830730545455 4.007073142193004 3 0.36660346266731425 1.0099121996253002 4 0.15596181450616514 0.5728541605489766 5 0.07434606879324597 0.3413452113545062 6 0.0521252416179931 0.2871872351418524 7 0.03974900051365553 0.25549949960199403 8 0.026646882655971645 0.19575019235917274 9 0.019362069144712845 0.16001477206705206 >10 0.10391826798936749 1.7770984105411642 >50 0.008772123075475573 0.557489137907014 >100 0.007232644784992961 1.2228747259061696 >500 6.155442335763506E-4 0.41671568697582195 >1k 1.5388605839408766E-4 0.15925350261843044 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1127 0.15429119828267188 No Hit GTCCTAAAGTGTGTATTTCTCATTT 824 0.11280918135308042 No Hit CTGTAGGACGTGGAATATGGCAAGA 796 0.1089758596566165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.3690434630228205E-4 13 0.0 0.0 0.0 0.0 1.3690434630228205E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAGG 45 4.877372E-5 20.837124 1 TTATACC 45 1.2345767E-5 19.08404 4 TAGGACC 230 0.0 16.335524 4 TGTAGGA 760 0.0 15.543441 2 GTGTAAG 95 2.860961E-7 15.51034 1 GTCCTAG 105 5.3219992E-8 15.308907 1 CTGTAGG 715 0.0 14.987741 1 CAAGTCG 50 7.2882493E-4 14.629476 6 ATACCTA 45 0.0061216177 13.932834 6 GTAGTGC 45 0.0061216177 13.932834 6 GTAGGAC 805 0.0 13.905381 3 TCCTAGG 80 2.0557854E-4 13.736064 2 GTCTATA 90 4.5805908E-5 13.395294 1 CTCTTAT 60 0.007938567 13.395294 1 CTATACT 105 5.5028067E-8 13.29067 4 GTCCTAC 790 0.0 13.225733 1 CTTAGAG 75 5.3789023E-5 13.169214 3 TCTATAC 110 7.575363E-8 12.969681 3 TCCTACA 800 0.0 12.53416 2 ATCTAAT 100 9.712465E-5 12.362458 2 >>END_MODULE