FastQCFastQC Report
Thu 26 May 2016
SRR1926437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730437
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA21950.3005050401335091No Hit
GTCCTACAGTGGACATTTCTAAATT20720.28366580553832843No Hit
GTCCTAAAGTGTGTATTTCTCATTT17650.24163617122352782No Hit
CTTTAGGACGTGAAATATGGCGAGG17090.23396952783060004No Hit
GTCCTACAGTGTGCATTTCTCATTT13810.18906490224345152No Hit
CTGTAGGACCTGGAATATGGCGAGA11890.16277926775341336No Hit
CTGAAGGACCTGGAATATGGCGAGA9990.13676744195597978No Hit
GTACATGGGAAGCAGTGGTATCAAC9680.13252340722060904No Hit
CCCATGTACTCTGCGTTGATACCAC9500.13005912898716795No Hit
GTATCAACGCAGAGTACTTTTTTTT8140.11144013789005759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC2250.014.8227071
CCTATAC801.2300552E-714.3012033
AGCACTA400.005158529614.2982475
GACCTAC400.005170330414.2933251
GTCCTAT954.6802597E-914.0425641
TAGGACC4150.013.5517524
TCTAGAC852.5772715E-713.4599543
CGGTTTC500.001517576313.27735913
GAACTAG655.2917938E-513.1938381
TCCAACG1102.8467184E-912.92009618
TAATCTG603.972457E-412.7095535
ACGAAAT751.5021196E-512.64510412
ATAAGAC1155.0276867E-912.4358273
GTCCTAG1155.0695235E-912.4289781
TAGGGGT550.002989100312.1318454
GGTCTAC550.002996821412.1276681
TGTCGAG550.003127512812.05875718
TTAGATA657.7903125E-411.7318954
AACCAGT657.7903125E-411.7318956
GCCTTAG657.812857E-411.7278571
Produced by FastQC (version 0.11.5)