FastQCFastQC Report
Thu 26 May 2016
SRR1926436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561909
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12720.2263711739801285No Hit
CTGTAGGACGTGGAATATGGCAAGA9550.16995634524451467No Hit
GTCCTAAAGTGTGTATTTCTCATTT9440.16799873289091294No Hit
GTCCTACAGTGTGCATTTCTCATTT6680.11888045929145111No Hit
CTTTAGGACGTGAAATATGGCGAGG6650.1183465650131961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG300.00243946922.207781
GACGTGC250.004046217520.3540427
TAGCCTG350.001178016217.8377065
TAAGGCG350.001178016217.8377065
GCATTAG552.3652207E-416.958671
CTCCTAG500.00244734215.9896011
TATAGGA603.7997033E-415.9360722
GTCCTAA3350.015.1145491
GTCCTAC8750.014.7713461
GTATTGT550.004616139514.5360021
GTGTAAC550.004616139514.5360021
TAGGACC2450.014.4070454
TCCTACA9150.014.1819862
TTCTAGA700.001222505513.6594922
TCTTACA600.00697151713.6594912
TATTATA600.00697151713.6594912
ATTTAGA1501.7807906E-913.3246681
CTCTTAG802.657183E-413.3246681
TTTAGAA1756.366463E-1113.2692192
CTGCACA652.946312E-413.1644794
Produced by FastQC (version 0.11.5)