##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926436_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 561909 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.083559793489695 27.0 12.0 31.0 2.0 31.0 2 16.968263544452928 23.0 2.0 31.0 2.0 31.0 3 20.717941873150277 25.0 15.0 31.0 2.0 31.0 4 23.004372594138907 28.0 10.0 35.0 2.0 37.0 5 23.623474619555836 30.0 10.0 35.0 2.0 37.0 6 24.373672605350688 31.0 10.0 35.0 2.0 37.0 7 24.22672176455618 31.0 11.0 35.0 2.0 37.0 8 24.49199603494516 32.0 17.0 35.0 2.0 37.0 9 27.320455803341822 32.0 18.0 38.0 2.0 39.0 10 28.060806998998057 33.0 26.0 38.0 2.0 39.0 11 25.070570145699747 31.0 10.0 37.0 2.0 39.0 12 26.266728242473427 31.0 17.0 37.0 2.0 39.0 13 22.767764887197036 29.0 2.0 37.0 2.0 39.0 14 26.271723713270298 31.0 17.0 38.0 2.0 40.0 15 27.434365706902717 32.0 22.0 39.0 2.0 40.0 16 27.81284158111011 32.0 24.0 39.0 2.0 40.0 17 27.135468554516834 31.0 18.0 39.0 2.0 40.0 18 27.23114774812292 31.0 20.0 38.0 2.0 40.0 19 27.631794472058644 31.0 24.0 39.0 2.0 40.0 20 27.910499742840923 32.0 26.0 39.0 2.0 41.0 21 25.68101774486616 31.0 10.0 38.0 2.0 40.0 22 26.66708132455611 31.0 18.0 39.0 2.0 40.0 23 26.682222566287425 31.0 18.0 39.0 2.0 40.0 24 25.655488700127602 31.0 2.0 39.0 2.0 40.0 25 25.477436737977147 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.834023933985076 1101 2 10.860990628675975 1101 3 7.341594721422968 1101 4 8.75984385511596 1101 5 8.28457094840661 1101 6 7.942701481272273 1101 7 7.86301950277085 1101 8 6.105627060576463 1101 9 5.874920464635906 1101 10 5.35147412463013 1101 11 8.220966507222816 1101 12 5.661279710184946 1101 13 10.404736051275602 1101 14 7.558816226908448 1101 15 6.727251372567171 1101 16 6.262378747430951 1101 17 2.246013994900359 1101 18 4.885395886216898 1101 19 5.418993855836334 1101 20 3.328283014637009 1101 21 0.473736396976129 1101 22 4.513038402729865 1101 23 5.62208392028322 1101 24 7.178004349975602 1101 25 7.708066549661922 1102 1 5.9263097173354 1102 2 10.915819370964385 1102 3 6.9628687232516775 1102 4 8.444381790354377 1102 5 8.07897130994483 1102 6 6.951155590091073 1102 7 6.99999414254636 1102 8 7.1378638681099105 1102 9 5.9558903119280515 1102 10 5.0997583945646525 1102 11 7.9429961659685695 1102 12 5.398706272734209 1102 13 9.95662729553461 1102 14 7.380577884939534 1102 15 5.384108512421502 1102 16 5.750153710909281 1102 17 6.554730849517856 1102 18 6.715990007016902 1102 19 5.2778320990128265 1102 20 5.6373551015743395 1102 21 5.072892457470719 1102 22 6.006629507208107 1102 23 6.465835299698664 1102 24 8.105087135921856 1102 25 7.845014914857558 1103 1 4.7569532802304835 1103 2 9.404195092605825 1103 3 6.079109398315882 1103 4 7.381769248025808 1103 5 6.826703595202467 1103 6 6.509922189425623 1103 7 6.428648104668881 1103 8 6.239309048662594 1103 9 4.870341274046357 1103 10 4.165877407945327 1103 11 5.349727831320923 1103 12 3.741157555347762 1103 13 8.193989872653777 1103 14 6.519871024232671 1103 15 5.157358123399309 1103 16 5.087156574281316 1103 17 2.8401044584208748 1103 18 1.7073492864086397 1103 19 3.5848960673344017 1103 20 3.8929184352309782 1103 21 6.341754235460286 1103 22 1.9767032632859163 1103 23 4.235844113318027 1103 24 5.5841368675070875 1103 25 1.4385341635492246 1104 1 6.033727857005058 1104 2 10.728527902652921 1104 3 7.3339971746322306 1104 4 8.087944732772723 1104 5 7.761450508520809 1104 6 7.471954944315524 1104 7 7.371515854679487 1104 8 7.476024649663909 1104 9 6.13515098171321 1104 10 5.07873964223613 1104 11 8.315515518537186 1104 12 5.2008588579062085 1104 13 9.667684132062323 1104 14 6.945884007388514 1104 15 6.360094768993097 1104 16 5.947907985032721 1104 17 6.123998556600686 1104 18 3.9337674628268147 1104 19 4.864922797361132 1104 20 3.9090010013135448 1104 21 3.1674447611508114 1104 22 5.177091485102711 1104 23 5.442301748347088 1104 24 6.5591294424996605 1104 25 5.587097954970158 1105 1 3.7431355225102863 1105 2 9.756759195169927 1105 3 6.464124103452537 1105 4 5.9490198945447474 1105 5 2.23338513603035 1105 6 4.9766919610734455 1105 7 6.214455957549905 1105 8 3.267741092338543 1105 9 4.014045954336403 1105 10 3.546032657003014 1105 11 6.72824988057468 1105 12 4.431263702770984 1105 13 5.502303345601398 1105 14 4.059439673557421 1105 15 4.60005245938633 1105 16 4.663703571560017 1105 17 5.36702161948438 1105 18 2.406851086520195 1105 19 2.5469204744807605 1105 20 2.040882534414589 1105 21 2.9870731002913473 1105 22 4.447026776292358 1105 23 1.7984698253339815 1105 24 5.120656736344028 1105 25 5.678768769637486 1106 1 6.765272454539506 1106 2 11.509974085186848 1106 3 8.166033847169267 1106 4 9.255327929706318 1106 5 9.067774967238307 1106 6 7.7740630972213545 1106 7 7.964984206487216 1106 8 7.730655334425126 1106 9 6.741283790166534 1106 10 5.954141071843509 1106 11 8.054006143213453 1106 12 5.777405009361715 1106 13 4.624619799730404 1106 14 6.056720016919229 1106 15 6.128519093037436 1106 16 6.103419225061728 1106 17 6.2888903001001175 1106 18 6.329597774646974 1106 19 5.916808221581583 1106 20 5.6962743878255075 1106 21 5.900584717641024 1106 22 6.581426924862367 1106 23 6.747719753112445 1106 24 8.002711748518514 1106 25 6.974666586483675 1107 1 3.879081532455821 1107 2 -14.326574138163405 1107 3 -6.067399694795153 1107 4 1.4235995772347785 1107 5 4.3296989729551285 1107 6 4.614445532783918 1107 7 4.718761743326208 1107 8 5.164483139856102 1107 9 3.249139489737775 1107 10 2.4881610807144376 1107 11 5.455450133159729 1107 12 3.0513244810486704 1107 13 4.704399012189132 1107 14 3.807762350958953 1107 15 3.494055537766627 1107 16 3.351583435795554 1107 17 3.798934161910772 1107 18 -1.3119329532619481 1107 19 1.4844096212168871 1107 20 1.8970037752386482 1107 21 -1.1602944898894094 1107 22 1.8039886517363541 1107 23 -1.2340406332657494 1107 24 2.332222368990646 1107 25 3.11287564468682 1108 1 5.1061758391304615 1108 2 10.606954698591238 1108 3 -0.007173252482896686 1108 4 5.827634919697932 1108 5 7.16895245358036 1108 6 7.126263999315821 1108 7 7.158290447214448 1108 8 6.871700632667082 1108 9 5.5446827709685 1108 10 4.495675624840317 1108 11 7.075196927757762 1108 12 2.055915015266514 1108 13 2.9898757653138652 1108 14 4.814595480247451 1108 15 4.906060017262494 1108 16 4.857913570844765 1108 17 -0.023413017999825314 1108 18 3.5508530653962893 1108 19 4.46308646165383 1108 20 4.7533732956858366 1108 21 4.806768877894285 1108 22 1.567243498987441 1108 23 4.558426790739414 1108 24 5.2488515483508 1108 25 5.754931657366072 1109 1 3.43290565762171 1109 2 -14.326574138163405 1109 3 -6.139362097134031 1109 4 1.679906265587288 1109 5 1.5020245798759717 1109 6 2.2156269454900936 1109 7 3.2936198041729554 1109 8 3.56098280558815 1109 9 3.165513630369791 1109 10 2.23477399842465 1109 11 1.13465367739785 1109 12 1.4184774161208615 1109 13 2.5687999740825838 1109 14 3.1082243392046927 1109 15 3.009162453620906 1109 16 3.0056161042516365 1109 17 3.412810772134385 1109 18 3.434641049671626 1109 19 1.9839875455625702 1109 20 1.2832558831223828 1109 21 0.15675616916725588 1109 22 2.331686992370365 1109 23 2.656514051973577 1109 24 3.840013306836763 1109 25 4.015816204860041 1110 1 3.3374872555013013 1110 2 9.63800076062202 1110 3 6.6701204498532505 1110 4 7.850693638002831 1110 5 8.403927580321191 1110 6 4.357908840043855 1110 7 6.763121241773597 1110 8 7.460941612400422 1110 9 5.7157838826468605 1110 10 5.342537874079476 1110 11 3.0202333570896087 1110 12 5.87640211164495 1110 13 10.388868962269708 1110 14 7.735841042834274 1110 15 7.024301990343179 1110 16 6.54374372823689 1110 17 7.063542471332571 1110 18 6.922662778037921 1110 19 5.985541652190513 1110 20 5.929793366842755 1110 21 3.735287444782969 1110 22 5.936436275650511 1110 23 6.455397674976847 1110 24 7.447880165460909 1110 25 7.55815884896338 1111 1 5.081013571290775 1111 2 9.353425861836595 1111 3 7.35522166442815 1111 4 7.76143452869109 1111 5 8.441368875867749 1111 6 7.492261066764502 1111 7 7.293781160801938 1111 8 7.635269547623093 1111 9 5.965314247019329 1111 10 5.283821274889192 1111 11 7.197168579761673 1111 12 6.255450694640903 1111 13 8.370010662674567 1111 14 6.356577884939533 1111 15 5.490686605727792 1111 16 5.5486222707470105 1111 17 6.81467202598844 1111 18 6.5963388913982435 1111 19 5.367351368789706 1111 20 5.0458581441706905 1111 21 7.761016189722245 1111 22 6.812173117755773 1111 23 6.781648687122601 1111 24 6.578843728213947 1111 25 7.3171183631334245 1112 1 -2.496207534236863 1112 2 7.64428013319338 1112 3 5.806025684528652 1112 4 -0.7787715014596621 1112 5 3.741539730139106 1112 6 3.057612825558472 1112 7 5.597343974872288 1112 8 6.016158994859275 1112 9 4.585525302295711 1112 10 3.040358963331407 1112 11 -1.253087701645363 1112 12 3.5533150162489413 1112 13 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6.277978570141869 2204 5 1.5239491867485775 2204 6 4.458763657438077 2204 7 5.306542818833027 2204 8 5.461067475084235 2204 9 3.563003366190312 2204 10 3.316240964008365 2204 11 4.990914693751311 2204 12 4.951435150599451 2204 13 3.5902282792548768 2204 14 4.7235830662866825 2204 15 4.541759144587896 2204 16 4.373259576446493 2204 17 -0.6752295284157022 2204 18 2.8346186841443597 2204 19 3.6272840112767497 2204 20 4.073360734844265 2204 21 5.628140542053849 2204 22 4.929618713610694 2204 23 3.8593896197650857 2204 24 5.588367044276122 2204 25 5.97024271546503 2205 1 6.564207903274578 2205 2 11.110672825399348 2205 3 7.484897777788472 2205 4 8.38409444771943 2205 5 8.421134058053983 2205 6 7.947989811703774 2205 7 8.246926909789792 2205 8 8.066204770295158 2205 9 6.7512089838614315 2205 10 6.067234230354778 2205 11 8.197358863162481 2205 12 7.430045836341311 2205 13 10.788666533119912 2205 14 8.370456427449653 2205 15 7.00709551261038 2205 16 6.582205266698423 2205 17 7.307469596838647 2205 18 7.3356182436249675 2205 19 5.691007239235049 2205 20 6.2212913425512575 2205 21 -0.39831579408342677 2205 22 4.511335061075609 2205 23 5.812685124369562 2205 24 7.857799193800993 2205 25 8.209980711311559 2206 1 4.340428740951452 2206 2 9.020731251058152 2206 3 6.0518084907754535 2206 4 1.790179039669134 2206 5 6.089404804011462 2206 6 6.322390807283465 2206 7 6.347056609903735 2206 8 6.009726633451436 2206 9 4.802396083346675 2206 10 3.899088739959055 2206 11 3.846671573773648 2206 12 4.6429257445411025 2206 13 8.375228227544827 2206 14 0.3715958490113884 2206 15 2.672620737464321 2206 16 3.518153208870764 2206 17 4.631224920200019 2206 18 -0.7645074159869836 2206 19 1.4961318079114605 2206 20 2.915231397663703 2206 21 3.7321439342332177 2206 22 -1.3524596167751675 2206 23 -2.8520279595839853 2206 24 1.3550233689325104 2206 25 3.2389167663270335 2207 1 1.8718424918610381 2207 2 -14.326574138163405 2207 3 -6.305491166529082 2207 4 -4.229622497785488 2207 5 1.691890260796466 2207 6 2.5043690097380242 2207 7 3.619200712736763 2207 8 3.603849995688268 2207 9 1.1539048783838908 2207 10 0.8831267738708632 2207 11 -0.8142550454929101 2207 12 -1.4505065355016633 2207 13 1.4972489518802696 2207 14 -5.424921096730529 2207 15 -1.0232461844962408 2207 16 0.28158561086921097 2207 17 1.216225997475206 2207 18 1.2878989728646388 2207 19 0.8028483137998883 2207 20 0.6191697531319917 2207 21 -3.3475683316626856 2207 22 -4.085323827254413 2207 23 -0.610921577643186 2207 24 1.3094058463402192 2207 25 1.8377110311578626 2208 1 4.91768181773627 2208 2 6.683852402120955 2208 3 6.449013133622461 2208 4 8.244425050018105 2208 5 3.751918638900925 2208 6 6.144607038328484 2208 7 6.707529975967812 2208 8 6.887558647149159 2208 9 4.964167327588051 2208 10 4.4513046872114685 2208 11 6.681580902036554 2208 12 6.079028893693035 2208 13 8.861735781157979 2208 14 3.4402935247499613 2208 15 4.960539686296514 2208 16 5.380778668851278 2208 17 6.108145959637735 2208 18 0.47821566864942966 2208 19 3.4351665346664824 2208 20 4.429346295829454 2208 21 7.01118206649949 2208 22 5.997997762305534 2208 23 1.7385586397292343 2208 24 3.752426666602574 2208 25 4.92295248635617 2209 1 2.906191625121 2209 2 5.1258068142175475 2209 3 5.10816162302028 2209 4 0.3912129965999931 2209 5 5.023517944190729 2209 6 5.2651078576547725 2209 7 5.7818826100773 2209 8 4.21135443375146 2209 9 3.8132935430855817 2209 10 2.9468999498374338 2209 11 -0.3003821448760071 2209 12 2.8452540072703023 2209 13 2.3597870601900937 2209 14 3.328818050571005 2209 15 3.5196037899928037 2209 16 2.9958375916579847 2209 17 0.7161606388248813 2209 18 3.0369765725576663 2209 19 3.285937290114756 2209 20 3.5749430302990532 2209 21 5.719411634856815 2209 22 4.452291130178132 2209 23 0.22476255875821138 2209 24 -3.3129202469413315 2209 25 -0.12482159545870886 2210 1 -1.2784056426830261 2210 2 -14.326574138163405 2210 3 -7.513948641248707 2210 4 -1.3015392704355477 2210 5 0.11064398503805606 2210 6 -0.4948350031044946 2210 7 -0.28159438504959056 2210 8 -21.761730452376906 2210 9 -13.043148635076742 2210 10 -6.2444713015300195 2210 11 -9.87672224993265 2210 12 -3.9787196110359524 2210 13 -4.484329948679143 2210 14 -3.1794657831827386 2210 15 -5.057911647547318 2210 16 -4.541705240170021 2210 17 -3.9383978430071913 2210 18 -4.064298663186907 2210 19 -10.035046011122958 2210 20 -6.7289278820301845 2210 21 -4.999835338662038 2210 22 -9.38716749359794 2210 23 -7.4862028412616795 2210 24 -10.268038367855922 2210 25 -7.792012641233391 2211 1 0.11401145935835544 2211 2 5.083140750960144 2211 3 0.6910505979022759 2211 4 1.6687703259455766 2211 5 1.2309169223302625 2211 6 0.15494850076661493 2211 7 -21.515218839198063 2211 8 -13.647686101796124 2211 9 -9.1973404520303 2211 10 -7.076471227750723 2211 11 -9.924534693733575 2211 12 -4.709306433131008 2211 13 -7.199727457705581 2211 14 -4.7102352090414605 2211 15 -5.076786467133875 2211 16 -12.153019756708112 2211 17 -7.520522271794029 2211 18 -5.680269345138186 2211 19 -8.225581049372916 2211 20 -6.4393245379834845 2211 21 -4.14168180394196 2211 22 -5.63028411561276 2211 23 -8.149168630723228 2211 24 -23.055156271786053 2211 25 -22.861881636866578 2212 1 -0.9497691338090029 2212 2 -14.326574138163405 2212 3 -8.002893634906663 2212 4 -20.33756547130891 2212 5 -20.89263112413225 2212 6 -21.612738933235498 2212 7 -10.194819726116023 2212 8 -9.752861272776018 2212 9 -4.870654710852069 2212 10 -5.5336975721174575 2212 11 -6.156820333763825 2212 12 -2.918884116889032 2212 13 -6.55146605572898 2212 14 -3.9941449531979387 2212 15 -3.8525528621214313 2212 16 -4.072253560738577 2212 17 -4.334013118135726 2212 18 -3.4127165409742624 2212 19 -4.010544418349987 2212 20 -4.210780436760576 2212 21 -2.080923943315451 2212 22 -3.1921084786965572 2212 23 -3.4996639514583308 2212 24 -2.3899678017195356 2212 25 -2.7720811934075975 2213 1 -19.451986428709226 2213 2 -14.326574138163405 2213 3 -8.551660829576647 2213 4 -5.387925183539124 2213 5 -20.89263112413225 2213 6 -10.841755719806242 2213 7 -21.515218839198063 2213 8 -10.809692083072349 2213 9 -8.095534673843982 2213 10 -6.999552825830229 2213 11 -6.314301444219144 2213 12 -4.680700384495786 2213 13 -20.015989337325433 2213 14 -12.617024033525693 2213 15 -6.958634737197865 2213 16 -6.067696674097114 2213 17 -4.77218408911947 2213 18 -4.766797799249236 2213 19 -5.655615058068808 2213 20 -5.702698210745378 2213 21 -3.743552155601499 2213 22 -4.71199234987013 2213 23 -4.544068339256295 2213 24 -3.4028780943280204 2213 25 -4.31511904693852 2214 1 -19.451986428709226 2214 2 -14.326574138163405 2214 3 -12.59051098338676 2214 4 -20.33756547130891 2214 5 -20.89263112413225 2214 6 -21.612738933235498 2214 7 -16.96380495744999 2214 8 -15.446820426669964 2214 9 -14.139976241412548 2214 10 -14.19854119297713 2214 11 -14.96738411946712 2214 12 -12.609266529009483 2214 13 -20.015989337325433 2214 14 -17.350784582926792 2214 15 -24.860313394272207 2214 16 -18.87319006858461 2214 17 -24.592327974011557 2214 18 -18.443445170298414 2214 19 -15.436270739179447 2214 20 -14.090943912384581 2214 21 -11.489330853979558 2214 22 -12.657258758850913 2214 23 -12.563101955550922 2214 24 -11.290374780783482 2214 25 -11.04954230524704 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 86348.0 3 4.0 4 392.0 5 1577.0 6 2121.0 7 2175.0 8 1912.0 9 1794.0 10 1670.0 11 1542.0 12 1462.0 13 1597.0 14 2502.0 15 5689.0 16 8504.0 17 8906.0 18 9345.0 19 10649.0 20 12622.0 21 14788.0 22 15151.0 23 16477.0 24 17521.0 25 18099.0 26 15224.0 27 11910.0 28 12065.0 29 14272.0 30 13837.0 31 16113.0 32 21369.0 33 27026.0 34 33250.0 35 39061.0 36 39295.0 37 41626.0 38 34014.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64073908325721 18.989788766212403 12.084280993863452 26.285191156666933 2 13.362143426339223 20.104373895938167 43.386495477874185 23.146987199848425 3 18.766063449802957 24.57159426620749 29.184122674389045 27.478219609600508 4 11.001832269302506 17.33959427148808 39.251029110897484 32.40754434831193 5 11.507184158903941 39.12886296178377 37.08094118130373 12.283011698008565 6 30.583467957394344 38.57337998914691 18.805993733483863 12.037158319974886 7 26.79227454685889 31.051389319082702 23.76506059524883 18.39127553880958 8 23.177350797222704 40.118092820187336 20.713749927874904 15.99080645471506 9 26.34313108945181 15.061845403415882 20.55301887162202 38.04200463551029 10 13.660517896871724 29.802617452449738 36.498178465291595 20.038686185386943 11 33.16768478694797 21.660540369536236 26.590263706962137 18.581511136553658 12 23.925650557620816 24.242435650087053 33.4721189591078 18.35979483318432 13 28.658642317305784 22.1603572893714 25.853362487153568 23.327637906169247 14 18.89776727309524 23.66390557350507 28.301204906361356 29.137122247038327 15 20.973376591258404 34.79893674902404 23.122960835212584 21.104725824504968 16 19.86799840308834 28.228893113135328 31.22511692982745 20.67799155394888 17 19.911072531923974 29.7042648925124 30.385321840153974 19.99934073540965 18 19.666075036517967 27.430814481517125 33.81933673625995 19.083773745704963 19 22.679298055668337 26.798213560698336 27.81070961338927 22.711778770244056 20 22.71178575571797 28.551388108050226 29.438063877064764 19.298762259167045 21 20.68974493419756 26.900163994778943 27.252882924090127 25.157208146933375 22 21.69251734121806 30.424394458039494 28.253986040274278 19.629102160468168 23 20.156379715174428 29.664401791472333 29.60579057446119 20.57342791889205 24 22.31132582907103 27.33064066579954 30.356273165579495 20.00176033954994 25 21.17135013121302 28.43322922994971 29.977498703903965 20.417921934933307 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 568.0 1 568.0 2 1165.5 3 1763.0 4 1763.0 5 1763.0 6 2552.0 7 3341.0 8 3341.0 9 3341.0 10 3892.0 11 4443.0 12 4443.0 13 4443.0 14 5691.5 15 6940.0 16 6940.0 17 6940.0 18 10164.5 19 13389.0 20 13389.0 21 13389.0 22 19092.0 23 24795.0 24 24795.0 25 24795.0 26 32943.0 27 41091.0 28 41091.0 29 41091.0 30 50260.5 31 59430.0 32 59430.0 33 59430.0 34 64454.0 35 69478.0 36 69478.0 37 69478.0 38 70862.0 39 72246.0 40 72246.0 41 72246.0 42 71542.5 43 70839.0 44 70839.0 45 70839.0 46 68005.0 47 65171.0 48 65171.0 49 65171.0 50 58253.5 51 51336.0 52 51336.0 53 51336.0 54 42635.0 55 33934.0 56 33934.0 57 33934.0 58 27661.0 59 21388.0 60 21388.0 61 21388.0 62 16812.0 63 12236.0 64 12236.0 65 12236.0 66 9032.5 67 5829.0 68 5829.0 69 5829.0 70 4154.0 71 2479.0 72 2479.0 73 2479.0 74 1656.5 75 834.0 76 834.0 77 834.0 78 555.5 79 277.0 80 277.0 81 277.0 82 175.5 83 74.0 84 74.0 85 74.0 86 49.5 87 25.0 88 25.0 89 25.0 90 14.0 91 3.0 92 3.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.910631436762893 2 36.12809191523895 3 5.4834501671978915 4 13.361772101888384 5 13.360526348572455 6 14.40446762732044 7 9.219108432148266 8 7.470782635622494 9 0.10499920805682057 10 1.3713964360777278 11 5.492882299447063 12 5.4517724400214265 13 15.841533059623533 14 1.5135902788529816 15 2.8538428820325 16 2.8216312605777802 17 2.820029577743015 18 1.4365315380248405 19 1.3763794493414414 20 0.28723512170120075 21 4.286637160109555 22 1.5310308252759788 23 0.10375345474089222 24 9.01284727598241 25 0.10820257372635071 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 561909.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.36976548438443 #Duplication Level Percentage of deduplicated Percentage of total 1 96.89455780530952 87.56338465578953 2 2.1252981887323434 3.841253978002578 3 0.403017838990646 1.0926188278682425 4 0.18511580780048498 0.6691548855352885 5 0.08980252788588249 0.4057716692476047 6 0.05543025206633386 0.30055313279849444 7 0.042044621300239615 0.2659693796747686 8 0.027256768607608384 0.1970550229545361 9 0.01880338737445849 0.1529331936607664 >10 0.13487731754660168 2.246233769471022 >50 0.01292342366116829 0.7991678625748878 >100 0.009846394618666751 1.6309592595198343 >500 8.205328848768747E-4 0.5991430241786725 >1k 2.0513322121921867E-4 0.23580133872378445 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1272 0.2263711739801285 No Hit CTGTAGGACGTGGAATATGGCAAGA 955 0.16995634524451467 No Hit GTCCTAAAGTGTGTATTTCTCATTT 944 0.16799873289091294 No Hit GTCCTACAGTGTGCATTTCTCATTT 668 0.11888045929145111 No Hit CTTTAGGACGTGAAATATGGCGAGG 665 0.1183465650131961 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAGG 30 0.002439469 22.20778 1 GACGTGC 25 0.0040462175 20.354042 7 TAGCCTG 35 0.0011780162 17.837706 5 TAAGGCG 35 0.0011780162 17.837706 5 GCATTAG 55 2.3652207E-4 16.95867 1 CTCCTAG 50 0.002447342 15.989601 1 TATAGGA 60 3.7997033E-4 15.936072 2 GTCCTAA 335 0.0 15.114549 1 GTCCTAC 875 0.0 14.771346 1 GTATTGT 55 0.0046161395 14.536002 1 GTGTAAC 55 0.0046161395 14.536002 1 TAGGACC 245 0.0 14.407045 4 TCCTACA 915 0.0 14.181986 2 TTCTAGA 70 0.0012225055 13.659492 2 TCTTACA 60 0.006971517 13.659491 2 TATTATA 60 0.006971517 13.659491 2 ATTTAGA 150 1.7807906E-9 13.324668 1 CTCTTAG 80 2.657183E-4 13.324668 1 TTTAGAA 175 6.366463E-11 13.269219 2 CTGCACA 65 2.946312E-4 13.164479 4 >>END_MODULE