FastQCFastQC Report
Thu 26 May 2016
SRR1926436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561909
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA26700.4751659076469678No Hit
GTCCTACAGTGGACATTTCTAAATT25340.45096270036607355No Hit
CTTTAGGACGTGAAATATGGCGAGG20230.36002270830330174No Hit
GTCCTAAAGTGTGTATTTCTCATTT20230.36002270830330174No Hit
GTCCTACAGTGTGCATTTCTCATTT15040.26765899816518335No Hit
CTGTAGGACCTGGAATATGGCGAGA12350.21978647788164987No Hit
CTGAAGGACCTGGAATATGGCGAGA11300.20110017814272416No Hit
CCCATGTACTCTGCGTTGATACCAC7630.1357871114361934No Hit
GTATCAACGCAGAGTACTTTTTTTT7370.13116002769131657No Hit
GTCCTTCAGTGTGCATTTCTCATTT7120.12671090870585805No Hit
GAATATGGCAAGAAAACTGAAAATC7050.1254651553899297No Hit
GTACATGGGAAGCAGTGGTATCAAC6990.12439736683341963No Hit
ACCTGGAATATGGCGAGAAAACTGA5860.10428734901914721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4750.014.6549124
GCTAATA400.00513461214.3074392
GTATTAT602.473324E-514.3035961
CGGCGAG400.00536472514.21322718
AACCATC551.9566354E-413.8181827
TGTAGGA13650.013.696012
CTGTAGG13550.013.5822581
GACCTGC500.001455678813.3500231
TATAGGA1403.6379788E-1212.9448262
TTAATAC603.9573945E-412.7143073
GTCTTAC603.9573945E-412.7143071
GGCGAGG5100.012.6339819
TAGGACA3500.012.5326754
GTAGGAC14500.012.4950943
GTCCTAA6400.012.2176541
GCCGGTT701.1086146E-412.1903611
AGGACCT8450.012.1876795
GGACCTG8000.012.1569676
CTAAGGT550.00297925512.1363844
CCTCTGG550.00297925512.1363841
Produced by FastQC (version 0.11.5)