##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926436_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 561909 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.30571854161439 31.0 28.0 31.0 22.0 34.0 2 28.742823126164556 31.0 28.0 33.0 23.0 34.0 3 29.127495733294893 31.0 28.0 33.0 25.0 34.0 4 32.8744939127154 35.0 33.0 37.0 27.0 37.0 5 32.52566696742711 35.0 33.0 37.0 25.0 37.0 6 32.6552751424163 35.0 33.0 37.0 26.0 37.0 7 32.475699801925224 35.0 33.0 37.0 25.0 37.0 8 32.446636377064614 35.0 33.0 37.0 25.0 37.0 9 33.65952316122362 37.0 33.0 39.0 22.0 39.0 10 33.52660484170924 37.0 33.0 39.0 21.0 39.0 11 33.80490969178283 37.0 34.0 39.0 25.0 39.0 12 33.58827674943807 37.0 33.0 39.0 23.0 39.0 13 33.73924425485266 37.0 34.0 39.0 25.0 39.0 14 34.355141134952454 38.0 33.0 40.0 19.0 41.0 15 34.51360273638614 38.0 34.0 40.0 22.0 41.0 16 34.5074665114814 38.0 34.0 40.0 23.0 41.0 17 34.42550128223609 38.0 34.0 40.0 22.0 41.0 18 34.39729564751588 38.0 34.0 40.0 19.0 41.0 19 34.42546390963661 39.0 34.0 40.0 19.0 41.0 20 34.41835599714545 39.0 34.0 40.0 19.0 41.0 21 34.332633931828816 39.0 34.0 40.0 18.0 41.0 22 34.26565511497413 39.0 34.0 40.0 18.0 41.0 23 33.91193413880183 39.0 34.0 40.0 13.0 41.0 24 34.00421954444581 39.0 34.0 40.0 16.0 41.0 25 33.79495434314097 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5457110745426625 1101 2 0.7356810668582021 1101 3 0.6955126447636175 1101 4 0.6808672597159315 1101 5 0.7173594173123448 1101 6 0.3060096618514052 1101 7 0.6325511110533881 1101 8 0.6082838069630796 1101 9 0.8652827740470599 1101 10 1.0270300083325452 1101 11 0.6337478127356135 1101 12 0.6297412921297365 1101 13 0.499705993395942 1101 14 0.8947670955885201 1101 15 0.7600165059724944 1101 16 0.7229256360181111 1101 17 0.4669021986959194 1101 18 0.8899265005586869 1101 19 1.057435561623734 1101 20 1.1075263568039944 1101 21 0.8661559607563802 1101 22 0.7606414566901805 1101 23 0.9972033319528251 1101 24 0.9343636289794901 1101 25 0.9216351548040365 1102 1 0.31438862238867316 1102 2 0.35821204458786937 1102 3 0.1607471716635338 1102 4 0.16140830586460453 1102 5 0.36269252643592864 1102 6 0.08471569134199086 1102 7 0.41789094646942004 1102 8 0.41155026273245454 1102 9 0.778491530839041 1102 10 0.6235218264283731 1102 11 0.49865127992917735 1102 12 0.5480494193827852 1102 13 0.23014456868312294 1102 14 0.5259466140280225 1102 15 0.4158668039262352 1102 16 0.33809868040113145 1102 17 0.4445488371261703 1102 18 0.6601384838690763 1102 19 0.7556131567639852 1102 20 0.6871961378841931 1102 21 0.7793279331365426 1102 22 0.958123300955485 1102 23 0.9599228560676352 1102 24 0.8516638633487403 1102 25 0.965869986489075 1103 1 0.12116161476004095 1103 2 0.04384626673324732 1103 3 -0.1148112702235089 1103 4 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0.4551868661050804 2202 18 0.3920350882515109 2202 19 0.3917180015378605 2202 20 0.4117785049377929 2202 21 0.5466793682857443 2202 22 0.38372239439026146 2202 23 0.7207615131547058 2202 24 0.4648528708241102 2202 25 0.6302228476249354 2203 1 -0.37387038027195274 2203 2 -0.09128436839738896 2203 3 -0.36970952512176325 2203 4 -0.8128635345330011 2203 5 -0.6731907924088532 2203 6 -0.8201998799021766 2203 7 -0.39755341309258796 2203 8 -0.494625373666306 2203 9 -0.09721862783258217 2203 10 -0.29959224678712104 2203 11 -0.34788419946634264 2203 12 -0.17017235208442827 2203 13 -0.3851996881418671 2203 14 -0.1116265970745971 2203 15 -0.125904136424829 2203 16 -0.2687763580154865 2203 17 -0.2503902670421425 2203 18 -0.2996008977769975 2203 19 -0.2134120708819296 2203 20 -0.22970980944771924 2203 21 -0.4054318641993149 2203 22 -0.39352268659313694 2203 23 0.11259709190617428 2203 24 -0.3468556698563887 2203 25 -0.3542480293693089 2204 1 -0.14548063530189737 2204 2 0.04345308758358968 2204 3 -0.07754435005503524 2204 4 -0.37198248121671185 2204 5 0.02616770746778485 2204 6 0.0013464494161752327 2204 7 0.19835421986167745 2204 8 0.2600972784656719 2204 9 0.7606195616118256 2204 10 0.7016414072962718 2204 11 0.4710535122174804 2204 12 0.4794822247722266 2204 13 0.36291842862910073 2204 14 0.674041706987488 2204 15 0.7102755733300086 2204 16 0.5799204546709618 2204 17 0.37674675828140636 2204 18 0.5023617337193116 2204 19 0.6087308984112951 2204 20 0.43913257442057585 2204 21 0.5459487068703694 2204 22 0.4601233219751535 2204 23 1.0013484096730352 2204 24 0.9768506756116153 2204 25 0.8656766055812355 2205 1 0.7382149028659626 2205 2 0.5492899695453701 2205 3 0.6672491302579431 2205 4 0.4983296582391432 2205 5 0.4883669480962567 2205 6 0.5284646134991604 2205 7 0.47981045111338005 2205 8 0.4412063384811944 2205 9 0.8889837662645519 2205 10 0.6850327773115907 2205 11 0.8624098924066956 2205 12 0.9900840581719876 2205 13 0.6472327916590359 2205 14 1.1557989598246436 2205 15 1.136697297229233 2205 16 0.8850084536640637 2205 17 1.1108712784299257 2205 18 1.2572326195828367 2205 19 1.0670399332542857 2205 20 1.0412911194479477 2205 21 1.0008153973111007 2205 22 1.077129468269547 2205 23 1.2891617553129393 2205 24 1.1495426686472072 2205 25 1.2781590372482867 2206 1 0.18756519014565498 2206 2 0.3864355165081257 2206 3 0.4086428610150534 2206 4 0.3420733449547768 2206 5 0.5755973456008405 2206 6 0.2867031970607812 2206 7 0.6937509910860697 2206 8 0.6993762335089571 2206 9 1.0712366693652342 2206 10 1.1526461335949705 2206 11 0.8461044466181136 2206 12 0.8872128774564914 2206 13 0.7707287147925257 2206 14 1.0801910662999532 2206 15 0.9043340884241715 2206 16 0.8728041173973224 2206 17 0.8403697713226848 2206 18 0.9160586921340865 2206 19 0.7770874495577118 2206 20 0.8695980683032758 2206 21 1.014494516804909 2206 22 0.9437767405266513 2206 23 1.2207055503140296 2206 24 1.081637992170208 2206 25 1.303527329004858 2207 1 -0.9050418564536109 2207 2 0.07249923428641836 2207 3 0.286156672495963 2207 4 0.0476032220641045 2207 5 0.1922926718526199 2207 6 0.1673553347406127 2207 7 0.38799996767463796 2207 8 0.4445437396372256 2207 9 0.7160346497791537 2207 10 0.6504610943049158 2207 11 0.3176054364917249 2207 12 0.2084766635299644 2207 13 0.20655360269493173 2207 14 -4.867727759998573 2207 15 -2.0152544331071027 2207 16 -0.5404081545963635 2207 17 -0.6121244256194771 2207 18 0.19592462218843565 2207 19 0.10960571242098638 2207 20 0.45431254972739055 2207 21 0.23486843292378978 2207 22 0.26957321275531143 2207 23 0.5733891850811617 2207 24 0.28179775411271635 2207 25 0.39042888231371364 2208 1 0.31300719712695013 2208 2 0.5360150304659896 2208 3 0.6366957599565062 2208 4 0.5090871845741205 2208 5 0.7084160683449312 2208 6 0.6568728104406532 2208 7 0.6985433021984377 2208 8 0.6971967063074374 2208 9 0.9265847409944854 2208 10 0.8170405674760275 2208 11 0.6968276447715667 2208 12 0.6444406021019873 2208 13 0.6518215055143983 2208 14 1.1943324517754448 2208 15 0.6445191212022223 2208 16 0.5276404163633757 2208 17 0.5054651729365176 2208 18 0.645494607034145 2208 19 0.48006159605144916 2208 20 0.7674630019431063 2208 21 1.0344195955573525 2208 22 1.1120988640521148 2208 23 1.1551239173713839 2208 24 1.0284382305559916 2208 25 1.0461397345255676 2209 1 -0.40756132700637693 2209 2 -0.191322011913293 2209 3 0.31577889949709714 2209 4 0.6523162132554461 2209 5 0.4986518576946679 2209 6 0.6062070465047498 2209 7 0.5744492219535218 2209 8 0.7386703161511221 2209 9 1.0956102823876321 2209 10 0.8008218318878306 2209 11 0.905550771359934 2209 12 0.7723742317665057 2209 13 0.8607536338984119 2209 14 0.8748687915947002 2209 15 0.8394638289431384 2209 16 0.7475515170080413 2209 17 0.9039010937709548 2209 18 0.9559044664240162 2209 19 0.8302544075671534 2209 20 0.8047340076047931 2209 21 0.9125617364029779 2209 22 0.7606275110353238 2209 23 1.1443117540556145 2209 24 1.0638350886604542 2209 25 1.1310709994279833 2210 1 0.03500785939231221 2210 2 0.014899113331942715 2210 3 0.5495706124989859 2210 4 0.5863642391489208 2210 5 1.0004419794771948 2210 6 0.8982116212604154 2210 7 1.1000127034691616 2210 8 1.0452761367592203 2210 9 1.536328320124852 2210 10 1.4236870212519719 2210 11 1.2106896866184584 2210 12 1.1980954790655005 2210 13 1.1822070681295216 2210 14 1.4557565292604906 2210 15 1.145868486876175 2210 16 1.3292122618071929 2210 17 1.4571798193489016 2210 18 1.3626327928409694 2210 19 1.2281700006128062 2210 20 1.1690689954446256 2210 21 1.322956196066471 2210 22 1.6685673340121667 2210 23 1.8364718343398678 2210 24 1.5376364755527874 2210 25 1.5532549480026674 2211 1 -0.9384488851883681 2211 2 -0.1970226990565216 2211 3 0.35634724106171944 2211 4 0.4870400114106701 2211 5 0.7552549258995924 2211 6 0.858457260212198 2211 7 0.6349541180949814 2211 8 0.7295503098546803 2211 9 1.0512017655719745 2211 10 0.9323180463692111 2211 11 0.7698514615639311 2211 12 0.9517037478803729 2211 13 0.919034004951385 2211 14 0.8440719634286111 2211 15 0.624398711026906 2211 16 0.7649585624670578 2211 17 1.060844344878845 2211 18 1.1357291716607776 2211 19 0.9330700988314931 2211 20 0.9311307579398402 2211 21 1.088010631355111 2211 22 0.8474867439622287 2211 23 1.1725768868384492 2211 24 1.012241410465613 2211 25 1.042363279068823 2212 1 1.0304691826041967 2212 2 0.17531894698641537 2212 3 0.30841316519605044 2212 4 0.5539931859822573 2212 5 0.7562781126698113 2212 6 0.6148650551627597 2212 7 0.9163257971280885 2212 8 0.9430264054488902 2212 9 1.1909241237248054 2212 10 0.6418606092998829 2212 11 0.9060488593447644 2212 12 0.6692585422245187 2212 13 0.6597533263233473 2212 14 0.6940697391095441 2212 15 0.6314076574723302 2212 16 0.1323692443496256 2212 17 0.7818924908503675 2212 18 0.8707612603900401 2212 19 0.8033232263411705 2212 20 0.8706491811551729 2212 21 0.7517849027735437 2212 22 0.5487220605296628 2212 23 1.0329856464410696 2212 24 1.0137715078439555 2212 25 0.7277918819388987 2213 1 0.3056742956756011 2213 2 0.024088268557981962 2213 3 -0.5373572251435625 2213 4 -1.365870479626274 2213 5 -2.0517450918264615 2213 6 -0.8639882258487965 2213 7 -0.6797250783577802 2213 8 -0.49949939652593756 2213 9 -0.8205983656008087 2213 10 -0.9361116027309393 2213 11 -0.5024358827950053 2213 12 -0.9645368604777147 2213 13 -0.713884792006823 2213 14 -7.943301516879014 2213 15 -3.5825985211767666 2213 16 -2.0784013852664103 2213 17 -1.5180644393925746 2213 18 -5.283032334445846 2213 19 -3.1273770028324748 2213 20 -5.597317022378672 2213 21 -2.5037064912509237 2213 22 -1.9842449140060126 2213 23 -4.940425425145122 2213 24 -2.5693478060175927 2213 25 -1.9300056051268832 2214 1 0.3863706640307889 2214 2 0.23656815611323267 2214 3 -1.5900102184301694 2214 4 -1.96628166738558 2214 5 -0.4988732504820561 2214 6 -0.35241702008846687 2214 7 -0.3383467671250173 2214 8 0.017949883124892096 2214 9 -5.16533645687586 2214 10 -1.3705311621990433 2214 11 -0.9832573952508525 2214 12 -1.1575331061546947 2214 13 -1.4609210961254817 2214 14 -2.6187683413628733 2214 15 -1.889756393850348 2214 16 -1.509038880411687 2214 17 -1.8715996061178046 2214 18 -1.163276211323776 2214 19 -4.341718640062538 2214 20 -2.2831991649936256 2214 21 -4.012805638668208 2214 22 -5.703986858239826 2214 23 -5.987320556854662 2214 24 -5.444792437458968 2214 25 -3.3764494783059753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19672.0 3 19.0 4 384.0 5 2225.0 6 2906.0 7 2199.0 8 1674.0 9 1353.0 10 1226.0 11 1135.0 12 1084.0 13 999.0 14 1087.0 15 1037.0 16 1168.0 17 1131.0 18 1264.0 19 1618.0 20 1980.0 21 2598.0 22 3453.0 23 4158.0 24 5019.0 25 6106.0 26 6853.0 27 7639.0 28 8849.0 29 10545.0 30 12815.0 31 16070.0 32 21212.0 33 26844.0 34 35522.0 35 46584.0 36 62785.0 37 92541.0 38 148155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.59040196064462 19.643671364044398 8.67898719026517 31.08693948504581 2 12.761022120790265 21.461581460264114 41.29819451911403 24.479201899831594 3 18.4147047042592 25.15127509012417 29.19827317637634 27.235747029240287 4 11.618877739029196 17.914305703162135 38.44405161809446 32.02276493971421 5 12.279540033465057 39.42984798319627 36.02727046174659 12.263341521592082 6 31.472024093415573 38.88367889021669 17.696192175713307 11.948104840654423 7 27.870629944288698 31.46618445362266 22.430039875309408 18.23314572677923 8 23.67554127880741 40.79188389460335 19.851675605488502 15.680899221100733 9 26.400730598819912 15.36005536569182 19.75791779336325 38.48129624212501 10 14.527046870800486 30.374977083448286 34.72048926615416 20.37748677959706 11 34.120666243101496 22.203357554854357 25.75685579385546 17.91912040818869 12 24.381731720686535 24.615050579114847 32.381667651434796 18.62155004876382 13 29.902706666025992 23.001589225301604 25.512138086416126 21.583566022256274 14 19.547914733555718 24.29281532787046 27.436075991073704 28.72319394750012 15 21.306104289019398 35.18117102687311 22.278163374265883 21.23456130984161 16 20.23342356339329 28.82592361233465 30.801018692037864 20.1396341322342 17 20.106570608367253 30.903518716596572 28.967165645394665 20.02274502964151 18 20.087596592951694 28.127547188615647 32.542970738253125 19.241885480179537 19 23.523716811324416 27.235580644989067 27.049960758211856 22.190741785474664 20 23.511845439790427 28.768971212164356 28.433148722913522 19.286034625131695 21 21.278712389372657 27.48131814938006 27.04815192495582 24.191817536291463 22 22.63017677239553 30.71084463854468 26.688485146171352 19.97049344288844 23 20.620598709043637 30.26949203518719 28.46225990329395 20.647649352475224 24 23.43776972975859 27.40641211592707 29.339301127414775 19.816517026899565 25 21.32320357922724 29.295312942131197 28.836519792350717 20.544963686290842 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 122.0 1 122.0 2 129.5 3 137.0 4 137.0 5 137.0 6 240.5 7 344.0 8 344.0 9 344.0 10 571.5 11 799.0 12 799.0 13 799.0 14 1354.5 15 1910.0 16 1910.0 17 1910.0 18 3619.5 19 5329.0 20 5329.0 21 5329.0 22 9213.0 23 13097.0 24 13097.0 25 13097.0 26 20362.5 27 27628.0 28 27628.0 29 27628.0 30 38889.0 31 50150.0 32 50150.0 33 50150.0 34 57021.0 35 63892.0 36 63892.0 37 63892.0 38 67842.5 39 71793.0 40 71793.0 41 71793.0 42 74012.0 43 76231.0 44 76231.0 45 76231.0 46 77388.0 47 78545.0 48 78545.0 49 78545.0 50 72334.0 51 66123.0 52 66123.0 53 66123.0 54 55288.0 55 44453.0 56 44453.0 57 44453.0 58 36902.0 59 29351.0 60 29351.0 61 29351.0 62 23366.5 63 17382.0 64 17382.0 65 17382.0 66 13089.5 67 8797.0 68 8797.0 69 8797.0 70 6293.0 71 3789.0 72 3789.0 73 3789.0 74 2591.0 75 1393.0 76 1393.0 77 1393.0 78 931.0 79 469.0 80 469.0 81 469.0 82 299.0 83 129.0 84 129.0 85 129.0 86 84.5 87 40.0 88 40.0 89 40.0 90 22.5 91 5.0 92 5.0 93 5.0 94 2.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08204175409185473 2 0.031143832898209496 3 0.03274551573297456 4 0.029898079582281113 5 0.02295745396496586 6 0.3715904176654939 7 0.20661708568469273 8 0.22174409023525163 9 0.2265491387395468 10 0.01566089882881392 11 0.00213557711302008 12 0.00302540091011178 13 0.0 14 0.07510112847453947 15 0.0016016828347650597 16 0.00213557711302008 17 0.0049830132637135195 18 0.0026694713912750996 19 0.0014237180753467198 20 8.898237970916999E-4 21 0.0 22 0.0 23 0.0 24 7.118590376733599E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 561909.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.1360756360128 #Duplication Level Percentage of deduplicated Percentage of total 1 93.13295862902444 74.63309816901155 2 5.031454097852228 8.064019722892255 3 0.8039806647546228 1.9328356608200494 4 0.3015583171438347 0.9666280044522829 5 0.171200083881342 0.685965143540348 6 0.1100183550914796 0.5289863534976313 7 0.07596885964833232 0.42614923979322794 8 0.05163672987162213 0.3310371912470938 9 0.0385070739770267 0.2777225213470097 >10 0.2306730042611258 3.4548720699030135 >50 0.026803885026043202 1.4949785577304657 >100 0.0212197207915825 3.6182318472291475 >500 0.002457020302172046 1.2372226441076617 >1k 0.0015635583741094839 2.3482528744283004 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2670 0.4751659076469678 No Hit GTCCTACAGTGGACATTTCTAAATT 2534 0.45096270036607355 No Hit CTTTAGGACGTGAAATATGGCGAGG 2023 0.36002270830330174 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2023 0.36002270830330174 No Hit GTCCTACAGTGTGCATTTCTCATTT 1504 0.26765899816518335 No Hit CTGTAGGACCTGGAATATGGCGAGA 1235 0.21978647788164987 No Hit CTGAAGGACCTGGAATATGGCGAGA 1130 0.20110017814272416 No Hit CCCATGTACTCTGCGTTGATACCAC 763 0.1357871114361934 No Hit GTATCAACGCAGAGTACTTTTTTTT 737 0.13116002769131657 No Hit GTCCTTCAGTGTGCATTTCTCATTT 712 0.12671090870585805 No Hit GAATATGGCAAGAAAACTGAAAATC 705 0.1254651553899297 No Hit GTACATGGGAAGCAGTGGTATCAAC 699 0.12439736683341963 No Hit ACCTGGAATATGGCGAGAAAACTGA 586 0.10428734901914721 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 475 0.0 14.654912 4 GCTAATA 40 0.005134612 14.307439 2 GTATTAT 60 2.473324E-5 14.303596 1 CGGCGAG 40 0.005364725 14.213227 18 AACCATC 55 1.9566354E-4 13.818182 7 TGTAGGA 1365 0.0 13.69601 2 CTGTAGG 1355 0.0 13.582258 1 GACCTGC 50 0.0014556788 13.350023 1 TATAGGA 140 3.6379788E-12 12.944826 2 TTAATAC 60 3.9573945E-4 12.714307 3 GTCTTAC 60 3.9573945E-4 12.714307 1 GGCGAGG 510 0.0 12.63398 19 TAGGACA 350 0.0 12.532675 4 GTAGGAC 1450 0.0 12.495094 3 GTCCTAA 640 0.0 12.217654 1 GCCGGTT 70 1.1086146E-4 12.19036 11 AGGACCT 845 0.0 12.187679 5 GGACCTG 800 0.0 12.156967 6 CTAAGGT 55 0.002979255 12.136384 4 CCTCTGG 55 0.002979255 12.136384 1 >>END_MODULE