FastQCFastQC Report
Thu 26 May 2016
SRR1565905_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565905_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14301210
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT442990.30975700657496813No Hit
GTACATGGGAAGCAGTGGTATCAAC294110.20565392718518224No Hit
GGTATCAACGCAGAGTACTTTTTTT281520.19685047628837No Hit
TATCAACGCAGAGTACTTTTTTTTT258120.1804882244229684No Hit
CCCATGTACTCTGCGTTGATACCAC196170.13717021147161673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG14950.09.2119581
GTATTAG9950.09.0983331
GTATTAT13600.08.9933591
GGACCGT7400.08.9770466
GTATTAC9450.08.9682561
GGTATCA165950.08.7050471
TTATACT16300.08.6748654
TAGACTG16750.08.6684665
TTAGACA14500.08.639134
CTTACAC17150.08.5769263
GTCCTAC18900.08.5096521
ATAAGAC10600.08.5051463
GTCTTAT12450.08.4316831
TAGGACA17950.08.3532734
GTATTAA18500.08.329241
ACCGTGC9450.08.3281948
ATTATAC16050.08.277843
TGCGTTG62150.08.26314112
GTAGGAC17950.08.2475363
GTATCAA271650.08.210811
Produced by FastQC (version 0.11.5)