FastQCFastQC Report
Thu 26 May 2016
SRR1565905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565905_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14301210
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT511850.35790677851734226No Hit
GGTATCAACGCAGAGTACTTTTTTT352130.24622392091298567No Hit
TATCAACGCAGAGTACTTTTTTTTT343530.24021044373168424No Hit
GTACATGGGAAGCAGTGGTATCAAC307710.21516361203003104No Hit
CCCATGTACTCTGCGTTGATACCAC204020.14265925750338607No Hit
ACGCAGAGTACTTTTTTTTTTTTTT158670.11094865399501161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA181850.08.9639391
GTAGGAC19850.08.5716353
ATTATAC15200.08.5673823
TAGGACA19350.08.4493194
TTATACT16600.08.4175064
GTATTAG9050.08.3067911
GTATCAA295550.08.2973281
TAGGACG12750.08.2008094
TCTATAC12200.08.1029783
GTATAAT16100.07.97926241
GTATTAA19100.07.97152571
TTAGGAC15800.07.94123463
TAGACTG17300.07.85716065
GTTCTAG14800.07.84427741
TAGGACT14450.07.82804544
TAGGACC15100.07.8058274
GTATTAC10500.07.79406361
GTATAGA15700.07.75827461
GTCCTAA14750.07.74183751
TATACTG21250.07.73860845
Produced by FastQC (version 0.11.5)