FastQCFastQC Report
Thu 26 May 2016
SRR1565904_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565904_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16180813
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC456180.2819265014681277No Hit
GTATCAACGCAGAGTACTTTTTTTT443680.2742013024932678No Hit
CCCATGTACTCTGCGTTGATACCAC303220.1873947866525619No Hit
GGTATCAACGCAGAGTACTTTTTTT292890.18101068221973768No Hit
TATCAACGCAGAGTACTTTTTTTTT271590.16784694316657636No Hit
GAGTACATGGGAAGCAGTGGTATCA189100.1168668100916808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG11050.08.9811591
GTATTAT15800.08.5374551
GTCCTAG17700.08.4919771
TAGACTG16550.08.4863925
GTATAAG17550.08.4547561
GTCCTAC20700.08.4249181
GTATTAC11100.08.4198871
GTATAAT21750.08.4168921
GTCTTAC11100.08.3330831
GTCTTAG18150.08.2283461
TATACCG4550.08.1341585
GTAGGAC20700.08.1145363
GTCTTAT15750.08.0751551
ATACCGT4950.08.052026
GTATAGG18850.08.0250131
GTCCTAA17450.08.0062661
GTATTAA21050.07.9644281
TCTTATA16850.07.8845972
TAGGACC14300.07.83081054
TAGGACG13950.07.8231994
Produced by FastQC (version 0.11.5)