FastQCFastQC Report
Thu 26 May 2016
SRR1565904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16180813
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT540140.3338151179424668No Hit
GTACATGGGAAGCAGTGGTATCAAC490640.3032233300020215No Hit
GGTATCAACGCAGAGTACTTTTTTT378200.2337336201833616No Hit
TATCAACGCAGAGTACTTTTTTTTT359790.22235594713318793No Hit
CCCATGTACTCTGCGTTGATACCAC331270.20473013315214753No Hit
GAGTACATGGGAAGCAGTGGTATCA205350.1269095687589987No Hit
ACGCAGAGTACTTTTTTTTTTTTTT164410.1016079970765375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC11350.08.3845991
GTCTTAT12150.08.3808031
TAGGACA21400.08.3509444
GTATAAA22450.08.3507991
TGCGTTG90700.08.241158512
GGTATCA248050.08.1833251
TTAGGAC15750.08.1478563
GTCCTAG17850.08.0503891
GTCCTAC18700.08.0406961
TAGGACG12950.08.0010734
TTATACT19850.07.99737454
TATACTG25250.07.98115875
GTCTTAG18750.07.9177451
CTGCGTT95100.07.88034911
TAGACTG17750.07.872465
GTAGGAC20500.07.83651543
CCATGTA101100.07.80399662
GTATCAA375900.07.7316981
CGTACAC6150.07.7283193
CGAATTT3201.0913936E-117.71546815
Produced by FastQC (version 0.11.5)