FastQCFastQC Report
Thu 26 May 2016
SRR1565903_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565903_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16072267
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC542410.3374819494972302No Hit
GTATCAACGCAGAGTACTTTTTTTT458770.28544199769702683No Hit
CCCATGTACTCTGCGTTGATACCAC355170.22098313822188245No Hit
GGTATCAACGCAGAGTACTTTTTTT300710.1870986837140025No Hit
TATCAACGCAGAGTACTTTTTTTTT270710.1684329908157947No Hit
GAGTACATGGGAAGCAGTGGTATCA228540.1421952484985472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT16700.08.8801161
GTATTAA19100.08.772641
GTCTTAT12800.08.6517161
GTATAAG16800.08.5979791
GTCCTAC19150.08.5485911
TAGGACC15500.08.5105324
GTATAGA19300.08.4821511
ACCGTGC10900.08.4371248
ATAAGAC11850.08.4090043
GTAGGAC20100.08.40422253
GTATAAA21900.08.3985371
GTCCTAG18450.08.2466041
GTATTAG11700.08.2305441
TTATACT16900.08.1986024
GTCCTAA15750.08.1929181
GTGTTAG14950.08.1804451
TAGGACA20500.08.1476514
GTCTTAG18050.08.1092511
GTATAAT20200.07.9612171
GTATTAC11150.07.9456121
Produced by FastQC (version 0.11.5)