FastQCFastQC Report
Thu 26 May 2016
SRR1565903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16072267
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC582040.36213932981576274No Hit
GTATCAACGCAGAGTACTTTTTTTT557840.34708233754454176No Hit
CCCATGTACTCTGCGTTGATACCAC389950.24262289818853805No Hit
GGTATCAACGCAGAGTACTTTTTTT380630.23682408959482815No Hit
TATCAACGCAGAGTACTTTTTTTTT363070.22589843735174384No Hit
GAGTACATGGGAAGCAGTGGTATCA257450.16018275455478684No Hit
ACGCAGAGTACTTTTTTTTTTTTTT170370.10600246996892225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC9400.08.6901928
TCTATAC14800.08.5422153
GTCCTAA15850.08.5248411
GTATTAG10350.08.4581371
TTATACT19550.08.3144334
TAAGACT17750.08.3007374
CGTGCGC7050.08.21860610
GTAGGAC19600.08.1476773
TGCGTTG100700.08.13976212
TAATACT20350.08.03428654
GTCCTAG18950.07.98390051
GTCTTAG19300.07.93772031
CTTATAC13900.07.93275073
CTGCGTT103750.07.928479711
CTAATAC12900.07.8845093
GTATAGG18350.07.8301151
TAAGGCT20300.07.81994534
CTATACT17900.07.80634264
TAGGACT16950.07.7952214
ATAATAC16500.07.7197183
Produced by FastQC (version 0.11.5)