FastQCFastQC Report
Thu 26 May 2016
SRR1565902_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565902_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14804208
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC839210.5668726081125042No Hit
CCCATGTACTCTGCGTTGATACCAC554230.37437328629805794No Hit
GTATCAACGCAGAGTACTTTTTTTT444440.3002119397403765No Hit
GAGTACATGGGAAGCAGTGGTATCA353550.23881723358655862No Hit
GGTATCAACGCAGAGTACTTTTTTT289130.19530257883434224No Hit
TATCAACGCAGAGTACTTTTTTTTT260570.176010766668504No Hit
GTATCAACGCAGAGTACATGGGAAG212920.1438239722111443No Hit
GCGTTGATACCACTGCTTCCCATGT195910.1323339958476671No Hit
CATGTACTCTGCGTTGATACCACTG192020.12970636456877668No Hit
TATCAACGCAGAGTACATGGGAAGC176030.11890538149693655No Hit
ACGCAGAGTACATGGGAAGCAGTGG165370.11170472611570981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC24100.09.5956391
GTATTAT15750.08.6261751
TACACCG5300.08.5947685
GTAGGAC27250.08.4975183
GTATTAC11150.08.4689751
GTCCTAA17700.08.4379731
GTCTTAT12200.08.3719321
TGCGTTG163550.08.31738212
CCATGTA163800.08.3137962
GTATAAT19650.08.2871251
GTATTAA18750.08.2737871
CTGCGTT165900.08.20422911
GTCTTAA16250.08.1828651
GTCCTAG16400.08.1667761
TAGGACG19850.08.1275034
GTATAAG15550.08.1174681
TAGGACC16500.08.1096844
TAGGACT15450.08.1080094
ATTATAC15100.08.1073983
GTCTTAG19950.08.017611