FastQCFastQC Report
Thu 26 May 2016
SRR1565902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14804208
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC902160.6093943019444201No Hit
CCCATGTACTCTGCGTTGATACCAC600110.40536447474934156No Hit
GTATCAACGCAGAGTACTTTTTTTT543740.3672874631321041No Hit
GAGTACATGGGAAGCAGTGGTATCA398380.2690991642376276No Hit
GGTATCAACGCAGAGTACTTTTTTT376630.2544073955188957No Hit
TATCAACGCAGAGTACTTTTTTTTT357720.24163400027883966No Hit
GTATCAACGCAGAGTACATGGGAAG229590.1550842841440758No Hit
CATGTACTCTGCGTTGATACCACTG216750.1464110744728796No Hit
TATCAACGCAGAGTACATGGGAAGC214050.14458726870089908No Hit
ACGCAGAGTACATGGGAAGCAGTGG199210.13456309179119882No Hit
GCGTTGATACCACTGCTTCCCATGT197640.13350258250897312No Hit
ACGCAGAGTACTTTTTTTTTTTTTT170270.11501459585004481No Hit
GGTATCAACGCAGAGTACATGGGAA164820.1113332101251212No Hit
GTACTTTTTTTTTTTTTTTTTTTTT153510.10369349039138062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG4400.09.0738495
GTATTAA18900.08.66013051
GTATTAG9150.08.5280661
TAGACAG23550.08.5169965
ACCGTGC7950.08.4828958
TAACCCG2701.4551915E-118.4497215
GTAGGAC27550.08.4189943
GTCCTAC27200.08.361551
GTATAAG14800.08.3587131
TAGGACG22350.08.293774
CTAGACA16300.08.2812464
ATTACAC17450.08.2801883
TAGGACC16100.08.2660314
TCTATAC13900.08.2065093
TGCGTTG156850.08.18082812
GTCCTAA17250.08.1645151
TTACACA26400.08.1016514
TAATACT18300.08.05147654
CTGCGTT159600.08.04041111
GCGTTAG1908.9498644E-78.01353551
Produced by FastQC (version 0.11.5)