FastQCFastQC Report
Thu 26 May 2016
SRR1565901_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565901_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16880436
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC511130.30279431171090604No Hit
GTATCAACGCAGAGTACTTTTTTTT446160.26430596934818507No Hit
CCCATGTACTCTGCGTTGATACCAC333160.19736457044119005No Hit
GGTATCAACGCAGAGTACTTTTTTT286770.16988305278370772No Hit
TATCAACGCAGAGTACTTTTTTTTT269260.15951009796192467No Hit
GAGTACATGGGAAGCAGTGGTATCA207280.122793036862318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT1151.0360309E-59.90660412
GTCCTAA16850.09.0208971
GTATTAC13750.08.5359071
GTCCTAC23450.08.4921961
GTAGGAC22500.08.3519593
ATTATAC16150.08.3449083
CTAGACA17350.08.3147624
TAATACT19300.08.3106534
GTATAGA19400.08.28146651
GTCCTAG18400.08.2609841
TACCGTA5400.08.2607147
GTCTTAT15100.08.218741
TCTATAC14450.08.210093
TAGGACA19250.08.1843294
GTAATAC11750.08.1581043
TATACTG22800.08.1171865
TAGGACC16650.08.094314
ACCGTAT5750.08.0793098
GTCCTAT14350.08.0449231
TGCGTTG100250.08.0306912
Produced by FastQC (version 0.11.5)