FastQCFastQC Report
Thu 26 May 2016
SRR1565901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16880436
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC544800.3227404789781496No Hit
GTATCAACGCAGAGTACTTTTTTTT514790.3049625021533804No Hit
CCCATGTACTCTGCGTTGATACCAC373850.22146939806531063No Hit
GGTATCAACGCAGAGTACTTTTTTT350590.20769013312215398No Hit
TATCAACGCAGAGTACTTTTTTTTT347350.20577075141897994No Hit
GAGTACATGGGAAGCAGTGGTATCA229750.13610430441488597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG4550.09.609945
TCTATAC14150.09.0016323
GTATAAT22900.08.8924261
GTATAAG18300.08.7877471
GTCTTAG18250.08.65541
GTATTAG11300.08.5894171
GTCTTAA15200.08.5766741
AAGGCGT12650.08.4106346
GTATTAA21050.08.40819551
GTCTTAT16100.08.3927531
TACCGTA5300.08.2441027
TAATACT21650.08.1663984
TAGACTG20150.08.1610355
GTCCTAC24350.08.1284241
TAGGACC15750.08.0872294
GTATAGA19100.08.07093051
CTAATAC13550.07.99720953
CGTGCGC9050.07.97673310
TAGGACG16600.07.9021724
CTTATAC14100.07.88750553
Produced by FastQC (version 0.11.5)