FastQCFastQC Report
Thu 26 May 2016
SRR1565900_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565900_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13242602
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1114140.8413301253031693No Hit
CCCATGTACTCTGCGTTGATACCAC722960.545935005824384No Hit
GAGTACATGGGAAGCAGTGGTATCA459350.3468729181772585No Hit
GTATCAACGCAGAGTACTTTTTTTT410130.30970499604231855No Hit
GTATCAACGCAGAGTACATGGGAAG274820.20752719140845582No Hit
GGTATCAACGCAGAGTACTTTTTTT269670.20363822759303646No Hit
TATCAACGCAGAGTACTTTTTTTTT255480.1929228107889975No Hit
TATCAACGCAGAGTACATGGGAAGC242520.18313621446902958No Hit
GCGTTGATACCACTGCTTCCCATGT241530.18238862725014315No Hit
CATGTACTCTGCGTTGATACCACTG239740.18103692914730804No Hit
ACGCAGAGTACATGGGAAGCAGTGG221930.16758791059340153No Hit
GGTATCAACGCAGAGTACATGGGAA195920.14794675547902142No Hit
ACTCTGCGTTGATACCACTGCTTCC154890.11696341851850564No Hit
GTGGTATCAACGCAGAGTACATGGG136080.1027592613596633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC8400.09.1680141
GTATTAG9800.08.7423561
CCATGTA202350.08.3809832
GTCTTAA12300.08.3741981
CTAATAC9550.08.3476223
TGCGTTG204650.08.32180912
CTGCGTT205850.08.30469711
GTCTAGA13600.08.28154851
ACCGTCC4700.08.2705318
AGTGGTA284800.08.20684514
GTCCTAT9750.08.1947951
CTAGACA12550.08.1670814
TAGACAG17750.08.0735665
CGTATCA4900.08.0547551
TAATACT16500.08.0525044
TCTGCGT213850.07.99767310
TGGTATC295250.07.94479416
GTGGTAT296200.07.935862515
CTCTGCG219400.07.9267319
TAAGACT13900.07.92008834