FastQCFastQC Report
Thu 26 May 2016
SRR1565900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565900_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13242602
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1174470.8868876373389459No Hit
CCCATGTACTCTGCGTTGATACCAC802290.6058401513539409No Hit
GAGTACATGGGAAGCAGTGGTATCA497110.3753869518996342No Hit
GTATCAACGCAGAGTACTTTTTTTT428030.32322197707066935No Hit
GGTATCAACGCAGAGTACTTTTTTT294240.22219198311630903No Hit
GTATCAACGCAGAGTACATGGGAAG292390.2207949766971778No Hit
TATCAACGCAGAGTACTTTTTTTTT291640.22022862274347593No Hit
TATCAACGCAGAGTACATGGGAAGC286100.2160451548721316No Hit
CATGTACTCTGCGTTGATACCACTG276860.20906767416252486No Hit
GCGTTGATACCACTGCTTCCCATGT258870.19548273065972985No Hit
ACGCAGAGTACATGGGAAGCAGTGG256590.19376101464047624No Hit
GGTATCAACGCAGAGTACATGGGAA217930.1645673561736583No Hit
ACTCTGCGTTGATACCACTGCTTCC168880.12752780760155746No Hit
GTGGTATCAACGCAGAGTACATGGG151950.1147433110199944No Hit
ACGCAGAGTACTTTTTTTTTTTTTT133060.10047874277275719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT14050.08.8628934
TCTTATA12900.08.7686192
CTAATAC10900.08.6334153
TAGGACG7700.08.3945754
ATAGGAC10600.08.3397123
ACACCGT7600.08.2495186
GTCCTAG14450.08.1654691
TAATACT15600.08.1650854
TGCGTTG205600.08.14919412
GTACTAG6800.08.116081
AGTGGTA289750.08.08202514
GTAGGAC14000.08.0796563
TAGACAG18850.08.0684325
CCATGTA205800.08.0644142
CTGCGTT209250.08.03915311
GTATAAT17300.08.0303421
ATTATAC12350.08.0046133
ATTACAC14850.08.0012443
GTATAGA15800.07.94957831
AATACCG3001.8189894E-117.9213515