FastQCFastQC Report
Thu 26 May 2016
SRR1565899_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565899_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14652672
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC873250.5959663875639883No Hit
CCCATGTACTCTGCGTTGATACCAC558820.3813775398780509No Hit
GTATCAACGCAGAGTACTTTTTTTT474760.3240091636528819No Hit
GAGTACATGGGAAGCAGTGGTATCA370130.25260239224627423No Hit
GGTATCAACGCAGAGTACTTTTTTT307600.20992758180896973No Hit
TATCAACGCAGAGTACTTTTTTTTT282820.19301599053060084No Hit
GTATCAACGCAGAGTACATGGGAAG224080.15292773904991527No Hit
GCGTTGATACCACTGCTTCCCATGT195920.1337094012614218No Hit
CATGTACTCTGCGTTGATACCACTG194350.13263792433216276No Hit
TATCAACGCAGAGTACATGGGAAGC183960.12554706745636562No Hit
ACGCAGAGTACATGGGAAGCAGTGG173820.11862682792599193No Hit
GGTATCAACGCAGAGTACATGGGAA160320.10941349127312752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC27200.09.5998591
TAGGACC16450.09.2878194
CGCGATT1554.315625E-79.18669212
GTCCTAA17850.08.9065641
TATACTG22900.08.8266915
TAGGACG24850.08.8214384
TTAGGAC20150.08.8068443
GTCCTAG16100.08.7375861
GTAGGAC28700.08.7292273
TAATCCG2304.1472958E-108.6645355
GTATTAA18350.08.6638781
GTATAAA23150.08.6155661
GTCTTAG18850.08.5874141
GTCCTAT12250.08.5734361
GTATTAC12700.08.4972571
TGCGTTG161900.08.3436812
CCATGTA162200.08.325282
GTATAAT22400.08.3018281
GTCTTAT13700.08.2990031
CTGCGTT164000.08.281898511