FastQCFastQC Report
Thu 26 May 2016
SRR1565899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14652672
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC935330.6383340867795307No Hit
CCCATGTACTCTGCGTTGATACCAC610380.41656566119817595No Hit
GTATCAACGCAGAGTACTTTTTTTT561970.3835273184303859No Hit
GAGTACATGGGAAGCAGTGGTATCA407560.27814722120306795No Hit
GGTATCAACGCAGAGTACTTTTTTT390990.266838703548404No Hit
TATCAACGCAGAGTACTTTTTTTTT372310.25409017549836643No Hit
GTATCAACGCAGAGTACATGGGAAG240120.16387454793228157No Hit
TATCAACGCAGAGTACATGGGAAGC227390.15518671270332127No Hit
CATGTACTCTGCGTTGATACCACTG214650.14649205278054406No Hit
ACGCAGAGTACATGGGAAGCAGTGG203560.1389234673375614No Hit
GCGTTGATACCACTGCTTCCCATGT201420.13746298286073694No Hit
ACGCAGAGTACTTTTTTTTTTTTTT176290.12031252729877526No Hit
GGTATCAACGCAGAGTACATGGGAA174350.11898853669828956No Hit
GTACTTTTTTTTTTTTTTTTTTTTT158300.10803490312210633No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT1151.0311298E-59.91053412
CGTTCGG1502.7138994E-68.86487610
GTAGGAC27650.08.8013953
GTCTTAC11050.08.7845231
GTATAGA16600.08.7139641
GTCCTAA18800.08.7066461
TATACCG3200.08.6150475
GTATTAC12100.08.5727751
TATACTG21400.08.528985
TAGGACC17250.08.4867484
TAGGACG22600.08.4546824
TTAGGAC21850.08.4402833
GTCCTAC27000.08.3886781
CTAGACA13950.08.3137184
AGTGGTA238500.08.158414
TAGGACA21050.08.1288684
TGCGTTG158250.08.03619612
TAGACAG19150.07.9922075
CCATGTA161700.07.93652632
CTGCGTT160800.07.926935711