FastQCFastQC Report
Thu 26 May 2016
SRR1565898_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565898_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15234806
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT485170.3184615544169056No Hit
GTACATGGGAAGCAGTGGTATCAAC376290.24699362761823157No Hit
GGTATCAACGCAGAGTACTTTTTTT317040.20810242020804204No Hit
TATCAACGCAGAGTACTTTTTTTTT286350.18795775935709325No Hit
CCCATGTACTCTGCGTTGATACCAC236390.15516443071214692No Hit
GAGTACATGGGAAGCAGTGGTATCA161170.10579064807257801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT21450.09.2080281
GTCCTAC24300.09.1193031
GTCCTAA17050.09.04141
TAGACTG16450.08.9994545
CTAATAC11900.08.85180953
TAGGACG19350.08.8277014
GTAGGAC24600.08.7568263
GTCTTAG18900.08.5132371
GTATTAG11150.08.4682031
GTATAAG16900.08.3235041
GTACTAG8500.08.1611931
ACCGTCC5500.08.1010368
GTATTAT15700.08.1005471
GTATCAA311600.08.09491351
TCTATAC14450.08.0778043
CTTACAC21900.08.0598123
GTGTTAG17500.07.9830711
GTATTAA18850.07.9735791
GGTATCA193700.07.9584851
TTATACT20050.07.95153474
Produced by FastQC (version 0.11.5)