FastQCFastQC Report
Thu 26 May 2016
SRR1565898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15234806
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT565520.37120262640692636No Hit
GTACATGGGAAGCAGTGGTATCAAC405970.26647533286606995No Hit
GGTATCAACGCAGAGTACTTTTTTT395180.2593928665714549No Hit
TATCAACGCAGAGTACTTTTTTTTT378270.24829328315700244No Hit
CCCATGTACTCTGCGTTGATACCAC258590.16973632614685083No Hit
GAGTACATGGGAAGCAGTGGTATCA177630.11659485522821884No Hit
ACGCAGAGTACTTTTTTTTTTTTTT175430.11515079351847343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT850.0074568218.93886512
TAGGACG18750.08.9236724
GTAGGAC24650.08.870283
GTATTAG9500.08.8153811
GGTATCA213950.08.6381141
GTCCTAA17250.08.606321
AGGACGT22100.08.6033955
GTATTAA19150.08.5972461
TAGGACC17150.08.5921084
TTATACT18300.08.4677634
GTCCTAG18150.08.4417261
GTATAAG15050.08.4100231
GGACGTG21600.08.1798376
TTAGGAC18050.08.1635863
TAGATCG2451.382432E-98.1486445
CTTATAC12400.08.1266333
GTCTTAG19100.08.12151
TAATACC11850.08.1028014
GTATTAT15050.08.0938561
TAGACAG20000.08.0807395
Produced by FastQC (version 0.11.5)