FastQCFastQC Report
Thu 26 May 2016
SRR1565897_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1565897_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15705233
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC545130.3471008675898027No Hit
GTATCAACGCAGAGTACTTTTTTTT500000.31836522259809835No Hit
CCCATGTACTCTGCGTTGATACCAC348700.22202790623991378No Hit
GGTATCAACGCAGAGTACTTTTTTT338390.21546321534994098No Hit
TATCAACGCAGAGTACTTTTTTTTT312560.19901646795052325No Hit
GAGTACATGGGAAGCAGTGGTATCA224140.14271676198627553No Hit
GTACTTTTTTTTTTTTTTTTTTTTT163240.10393987787382715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC19200.09.1246451
GTCCTAA15000.09.1126121
GTATTAA22800.09.0349281
GTATTAG11150.08.8921791
GTATTAC10550.08.8504451
CGACCGT1752.6264934E-78.686714
GTATTAT13800.08.5796951
ACCGTGC9650.08.5475768
GTATAAG17050.08.5250781
GTCCTAG19600.08.4473051
TAGGACT16650.08.436174
GTATAAT19400.08.2862981
GTATAAA21550.08.218951
GTCTTAG18150.08.1675141
TGCGTTG99200.08.06752112
TCGATAC2252.2278982E-88.0143613
GTATAGA18200.07.93351461
TAAGACA19500.07.9332444
GTAGGAC23500.07.915643
CTGCGTT101600.07.90394511
Produced by FastQC (version 0.11.5)